Production of first generation adenovirus vectors: a review

In the past decade, adenovirus vectors have generated tremendous interest, especially in gene therapy applications. In the so-called 'first generation' adenovirus vectors, the transgenes are inserted in place of the E1 region, or less often the E3 region. Although second-generation and hel...

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Bibliographic Details
Published in:Gene therapy Vol. 7; no. 20; pp. 1707 - 1714
Main Authors: Danthinne, X, Imperiale, M J
Format: Journal Article
Language:English
Published: Basingstoke Nature Publishing Group 01.10.2000
Subjects:
Adenoviridae - genetics
Adenovirus
Adenoviruses
Animals
Biological and medical sciences
Biotechnology
Expression vectors
Fundamental and applied biological sciences. Psychology
Gene expression
Gene therapy
Gene Transfer Techniques
Genetic engineering
Genetic technics
Genetic Therapy - methods
Genetic Vectors
Health. Pharmaceutical industry
Humans
Industrial applications and implications. Economical aspects
Methods. Procedures. Technologies
Recombination, Genetic
Transfection
Transfection - methods
Transgenes
Vaccination
Vectors (cloning, transfer, expression). Insertion sequences and transposons
Virus
Construction
Adenoviridae
Production
Review
Method
Gene therapy
Vector
ISSN:0969-7128, 1476-5462
Online Access:Get full text
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Summary:In the past decade, adenovirus vectors have generated tremendous interest, especially in gene therapy applications. In the so-called 'first generation' adenovirus vectors, the transgenes are inserted in place of the E1 region, or less often the E3 region. Although second-generation and helper-dependent adenovirus vectors will probably prevail in the future in applications that require long-term gene expression, first generation adenovirus vectors will remain very useful in other settings, such as cancer and vaccination, or simply to transfect cell lines that are refractory to other transfection methods. Until a few years ago, the construction of first generation adenovirus vectors was a labor-intensive and time-consuming process. More than 20 methods have appeared that facilitate their construction and are reviewed below.
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ISSN:0969-7128
1476-5462
DOI:10.1038/sj.gt.3301301