Production of first generation adenovirus vectors: a review

In the past decade, adenovirus vectors have generated tremendous interest, especially in gene therapy applications. In the so-called 'first generation' adenovirus vectors, the transgenes are inserted in place of the E1 region, or less often the E3 region. Although second-generation and hel...

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Veröffentlicht in:	Gene therapy Jg. 7; H. 20; S. 1707 - 1714
Hauptverfasser:	Danthinne, X, Imperiale, M J
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	Basingstoke Nature Publishing Group 01.10.2000
Schlagworte:	Adenoviridae - genetics Adenovirus Adenoviruses Animals Biological and medical sciences Biotechnology Expression vectors Fundamental and applied biological sciences. Psychology Gene expression Gene therapy Gene Transfer Techniques Genetic engineering Genetic technics Genetic Therapy - methods Genetic Vectors Health. Pharmaceutical industry Humans Industrial applications and implications. Economical aspects Methods. Procedures. Technologies Recombination, Genetic Transfection Transfection - methods Transgenes Vaccination Vectors (cloning, transfer, expression). Insertion sequences and transposons Virus Construction Adenoviridae Production Review Method Gene therapy Vector
ISSN:	0969-7128, 1476-5462
Online-Zugang:	Volltext
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Zusammenfassung:	In the past decade, adenovirus vectors have generated tremendous interest, especially in gene therapy applications. In the so-called 'first generation' adenovirus vectors, the transgenes are inserted in place of the E1 region, or less often the E3 region. Although second-generation and helper-dependent adenovirus vectors will probably prevail in the future in applications that require long-term gene expression, first generation adenovirus vectors will remain very useful in other settings, such as cancer and vaccination, or simply to transfect cell lines that are refractory to other transfection methods. Until a few years ago, the construction of first generation adenovirus vectors was a labor-intensive and time-consuming process. More than 20 methods have appeared that facilitate their construction and are reviewed below.
Bibliographie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 ObjectType-Article-2 ObjectType-Feature-1 ObjectType-Review-3 content type line 23
ISSN:	0969-7128 1476-5462
DOI:	10.1038/sj.gt.3301301