An Electrochemical Molecularly Imprinted Polymer Sensor for Rapid β-Lactoglobulin Detection
Facile detection of β-lactoglobulin is extraordinarily important for the management of the allergenic safety of cow’s milk and its dairy products. A sensitive electrochemical sensor based on a molecularly imprinted polymer-modified carbon electrode for the detection of β-lactoglobulin was successful...
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| Vydáno v: | Sensors (Basel, Switzerland) Ročník 21; číslo 24; s. 8240 |
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| Médium: | Journal Article |
| Jazyk: | angličtina |
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MDPI AG
09.12.2021
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| ISSN: | 1424-8220, 1424-8220 |
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| Abstract | Facile detection of β-lactoglobulin is extraordinarily important for the management of the allergenic safety of cow’s milk and its dairy products. A sensitive electrochemical sensor based on a molecularly imprinted polymer-modified carbon electrode for the detection of β-lactoglobulin was successfully synthesized. This molecularly imprinted polymer was prepared using a hydrothermal method with choline chloride as a functional monomer, β-lactoglobulin as template molecule and ethylene glycol dimethacrylate as crosslinking agent. Then, the molecularly imprinted polymer was immobilized on polyethyleneimine (PEI)-reduced graphene oxide (rGO)-gold nanoclusters (Au-NCs) to improve the sensor’s selectivity for β-lactoglobulin. Under optimal experimental conditions, the designed sensor showed a good response to β-lactoglobulin, with a linear detection range between 10−9 and 10−4 mg/mL, and a detection limit of 10−9 mg/mL (S/N = 3). The developed electrochemical sensor showed a high correlation in the detection of β-lactoglobulin in four different milk samples from the market, indicating that the sensor can be used with actual sample. |
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| AbstractList | Facile detection of β-lactoglobulin is extraordinarily important for the management of the allergenic safety of cow’s milk and its dairy products. A sensitive electrochemical sensor based on a molecularly imprinted polymer-modified carbon electrode for the detection of β-lactoglobulin was successfully synthesized. This molecularly imprinted polymer was prepared using a hydrothermal method with choline chloride as a functional monomer, β-lactoglobulin as template molecule and ethylene glycol dimethacrylate as crosslinking agent. Then, the molecularly imprinted polymer was immobilized on polyethyleneimine (PEI)-reduced graphene oxide (rGO)-gold nanoclusters (Au-NCs) to improve the sensor’s selectivity for β-lactoglobulin. Under optimal experimental conditions, the designed sensor showed a good response to β-lactoglobulin, with a linear detection range between 10−9 and 10−4 mg/mL, and a detection limit of 10−9 mg/mL (S/N = 3). The developed electrochemical sensor showed a high correlation in the detection of β-lactoglobulin in four different milk samples from the market, indicating that the sensor can be used with actual sample. Facile detection of β-lactoglobulin is extraordinarily important for the management of the allergenic safety of cow's milk and its dairy products. A sensitive electrochemical sensor based on a molecularly imprinted polymer-modified carbon electrode for the detection of β-lactoglobulin was successfully synthesized. This molecularly imprinted polymer was prepared using a hydrothermal method with choline chloride as a functional monomer, β-lactoglobulin as template molecule and ethylene glycol dimethacrylate as crosslinking agent. Then, the molecularly imprinted polymer was immobilized on polyethyleneimine (PEI)-reduced graphene oxide (rGO)-gold nanoclusters (Au-NCs) to improve the sensor's selectivity for β-lactoglobulin. Under optimal experimental conditions, the designed sensor showed a good response to β-lactoglobulin, with a linear detection range between 10 and 10 mg/mL, and a detection limit of 10 mg/mL (S/N = 3). The developed electrochemical sensor showed a high correlation in the detection of β-lactoglobulin in four different milk samples from the market, indicating that the sensor can be used with actual sample. Facile detection of β-lactoglobulin is extraordinarily important for the management of the allergenic safety of cow's milk and its dairy products. A sensitive electrochemical sensor based on a molecularly imprinted polymer-modified carbon electrode for the detection of β-lactoglobulin was successfully synthesized. This molecularly imprinted polymer was prepared using a hydrothermal method with choline chloride as a functional monomer, β-lactoglobulin as template molecule and ethylene glycol dimethacrylate as crosslinking agent. Then, the molecularly imprinted polymer was immobilized on polyethyleneimine (PEI)-reduced graphene oxide (rGO)-gold nanoclusters (Au-NCs) to improve the sensor's selectivity for β-lactoglobulin. Under optimal experimental conditions, the designed sensor showed a good response to β-lactoglobulin, with a linear detection range between 10-9 and 10-4 mg/mL, and a detection limit of 10-9 mg/mL (S/N = 3). The developed electrochemical sensor showed a high correlation in the detection of β-lactoglobulin in four different milk samples from the market, indicating that the sensor can be used with actual sample.Facile detection of β-lactoglobulin is extraordinarily important for the management of the allergenic safety of cow's milk and its dairy products. A sensitive electrochemical sensor based on a molecularly imprinted polymer-modified carbon electrode for the detection of β-lactoglobulin was successfully synthesized. This molecularly imprinted polymer was prepared using a hydrothermal method with choline chloride as a functional monomer, β-lactoglobulin as template molecule and ethylene glycol dimethacrylate as crosslinking agent. Then, the molecularly imprinted polymer was immobilized on polyethyleneimine (PEI)-reduced graphene oxide (rGO)-gold nanoclusters (Au-NCs) to improve the sensor's selectivity for β-lactoglobulin. Under optimal experimental conditions, the designed sensor showed a good response to β-lactoglobulin, with a linear detection range between 10-9 and 10-4 mg/mL, and a detection limit of 10-9 mg/mL (S/N = 3). The developed electrochemical sensor showed a high correlation in the detection of β-lactoglobulin in four different milk samples from the market, indicating that the sensor can be used with actual sample. |
| Author | Hong, Jingyi Wang, Bixuan Jiang, Ling Zhu, Liying Liu, Chun |
| AuthorAffiliation | 1 School of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing 211816, China; 201961105021@njtech.edu.cn 2 State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, China; hjy1364828909@outlook.com (J.H.); 201961219027@njtech.edu.cn (C.L.); jiangling@njtech.edu.cn (L.J.) |
| AuthorAffiliation_xml | – name: 2 State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, China; hjy1364828909@outlook.com (J.H.); 201961219027@njtech.edu.cn (C.L.); jiangling@njtech.edu.cn (L.J.) – name: 1 School of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing 211816, China; 201961105021@njtech.edu.cn |
| Author_xml | – sequence: 1 givenname: Bixuan surname: Wang fullname: Wang, Bixuan – sequence: 2 givenname: Jingyi surname: Hong fullname: Hong, Jingyi – sequence: 3 givenname: Chun surname: Liu fullname: Liu, Chun – sequence: 4 givenname: Liying surname: Zhu fullname: Zhu, Liying – sequence: 5 givenname: Ling orcidid: 0000-0001-6625-5557 surname: Jiang fullname: Jiang, Ling |
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| Keywords | molecularly imprinted polymer electrochemical sensor screen-printed carbon electrode β-lactoglobulin |
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| Snippet | Facile detection of β-lactoglobulin is extraordinarily important for the management of the allergenic safety of cow’s milk and its dairy products. A sensitive... Facile detection of β-lactoglobulin is extraordinarily important for the management of the allergenic safety of cow's milk and its dairy products. A sensitive... |
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| SubjectTerms | Allergens Allergies Carbon Composite materials Dairy products electrochemical sensor Electrochemical Techniques Electrodes Fourier transforms Graphene Lactoglobulins Limit of Detection Medical research Milk Molecular Imprinting molecularly imprinted polymer Molecularly Imprinted Polymers Polymers Proteins screen-printed carbon electrode Sensors Spectrum analysis Voltammetry β-lactoglobulin |
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| Title | An Electrochemical Molecularly Imprinted Polymer Sensor for Rapid β-Lactoglobulin Detection |
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