An Electrochemical Sensor Based on Gold-Nanocluster-Modified Graphene Screen-Printed Electrodes for the Detection of β-Lactoglobulin in Milk

A simple and low-cost electrochemical sensor based on multimodified screen-printed electrodes (SPEs) was successfully synthesized for the sensitive detection of β-lactoglobulin (β-Lg). The surface treatment of SPEs was accomplished by a simple drip coating method using polyethyleneimine (PEI), reduc...

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Vydané v:Sensors (Basel, Switzerland) Ročník 20; číslo 14; s. 3956
Hlavní autori: Hong, Jingyi, Wang, Yuxian, Zhu, Liying, Jiang, Ling
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: Switzerland MDPI AG 16.07.2020
MDPI
Predmet:
Allergens
Animals
Antibodies
Biosensing Techniques
Chromatography
Composite materials
Deoxyribonucleic acid
DNA
electrochemical sensors
Electrochemical Techniques - instrumentation
Electrodes
Food allergies
Fourier transforms
Gold
Graphene
Graphite
Lactoglobulins - analysis
Limit of Detection
Metal Nanoparticles
Methods
Milk - chemistry
PEI-rGO-AuNCs
Polymers
Proteins
screen-printed electrode
Sensors
Voltammetry
Work stations
β-lactoglobulin
United States > US
China
Japan
screen-printed electrode
electrochemical sensors
β-lactoglobulin
PEI-rGO-AuNCs
ISSN:1424-8220, 1424-8220
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Popis
Shrnutí:A simple and low-cost electrochemical sensor based on multimodified screen-printed electrodes (SPEs) was successfully synthesized for the sensitive detection of β-lactoglobulin (β-Lg). The surface treatment of SPEs was accomplished by a simple drip coating method using polyethyleneimine (PEI), reduced graphene oxide (rGO), and gold nanoclusters (AuNCs), and the treated SPEs showed excellent electrical conductivity. The modified SPEs were then characterized with UV-Vis, SEM, TEM, and FTIR to analyze the morphology and composition of the AuNCs and the rGO. An anti-β-Lg antibody was then immobilized on the composite material obtained by modifying rGO with PEI and AuNCs (PEI-rGO-AuNCs), leading to the remarkable reduction in conductivity of the SPEs due to the reaction between antigen and antibody. The sensor obtained using this novel approach enabled a limit of detection (LOD) of 0.08 ng/mL and a detection range from 0.01 to 100 ng/mL for β-Lg. Furthermore, pure milk samples from four milk brands were measured using electrochemical sensors, and the results were in excellent agreement with those from commercial enzyme-linked immunosorbent assay (ELISA) methods.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:1424-8220
1424-8220
DOI:10.3390/s20143956