An Electrochemical Sensor Based on Gold-Nanocluster-Modified Graphene Screen-Printed Electrodes for the Detection of β-Lactoglobulin in Milk

A simple and low-cost electrochemical sensor based on multimodified screen-printed electrodes (SPEs) was successfully synthesized for the sensitive detection of β-lactoglobulin (β-Lg). The surface treatment of SPEs was accomplished by a simple drip coating method using polyethyleneimine (PEI), reduc...

Full description

Saved in:
Bibliographic Details
Published in:Sensors (Basel, Switzerland) Vol. 20; no. 14; p. 3956
Main Authors: Hong, Jingyi, Wang, Yuxian, Zhu, Liying, Jiang, Ling
Format: Journal Article
Language:English
Published: Switzerland MDPI AG 16.07.2020
MDPI
Subjects:
Allergens
Animals
Antibodies
Biosensing Techniques
Chromatography
Composite materials
Deoxyribonucleic acid
DNA
electrochemical sensors
Electrochemical Techniques - instrumentation
Electrodes
Food allergies
Fourier transforms
Gold
Graphene
Graphite
Lactoglobulins - analysis
Limit of Detection
Metal Nanoparticles
Methods
Milk - chemistry
PEI-rGO-AuNCs
Polymers
Proteins
screen-printed electrode
Sensors
Voltammetry
Work stations
β-lactoglobulin
United States > US
China
Japan
screen-printed electrode
electrochemical sensors
β-lactoglobulin
PEI-rGO-AuNCs
ISSN:1424-8220, 1424-8220
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Abstract A simple and low-cost electrochemical sensor based on multimodified screen-printed electrodes (SPEs) was successfully synthesized for the sensitive detection of β-lactoglobulin (β-Lg). The surface treatment of SPEs was accomplished by a simple drip coating method using polyethyleneimine (PEI), reduced graphene oxide (rGO), and gold nanoclusters (AuNCs), and the treated SPEs showed excellent electrical conductivity. The modified SPEs were then characterized with UV-Vis, SEM, TEM, and FTIR to analyze the morphology and composition of the AuNCs and the rGO. An anti-β-Lg antibody was then immobilized on the composite material obtained by modifying rGO with PEI and AuNCs (PEI-rGO-AuNCs), leading to the remarkable reduction in conductivity of the SPEs due to the reaction between antigen and antibody. The sensor obtained using this novel approach enabled a limit of detection (LOD) of 0.08 ng/mL and a detection range from 0.01 to 100 ng/mL for β-Lg. Furthermore, pure milk samples from four milk brands were measured using electrochemical sensors, and the results were in excellent agreement with those from commercial enzyme-linked immunosorbent assay (ELISA) methods.
AbstractList A simple and low-cost electrochemical sensor based on multimodified screen-printed electrodes (SPEs) was successfully synthesized for the sensitive detection of β-lactoglobulin (β-Lg). The surface treatment of SPEs was accomplished by a simple drip coating method using polyethyleneimine (PEI), reduced graphene oxide (rGO), and gold nanoclusters (AuNCs), and the treated SPEs showed excellent electrical conductivity. The modified SPEs were then characterized with UV-Vis, SEM, TEM, and FTIR to analyze the morphology and composition of the AuNCs and the rGO. An anti-β-Lg antibody was then immobilized on the composite material obtained by modifying rGO with PEI and AuNCs (PEI-rGO-AuNCs), leading to the remarkable reduction in conductivity of the SPEs due to the reaction between antigen and antibody. The sensor obtained using this novel approach enabled a limit of detection (LOD) of 0.08 ng/mL and a detection range from 0.01 to 100 ng/mL for β-Lg. Furthermore, pure milk samples from four milk brands were measured using electrochemical sensors, and the results were in excellent agreement with those from commercial enzyme-linked immunosorbent assay (ELISA) methods.
A simple and low-cost electrochemical sensor based on multimodified screen-printed electrodes (SPEs) was successfully synthesized for the sensitive detection of β-lactoglobulin (β-Lg). The surface treatment of SPEs was accomplished by a simple drip coating method using polyethyleneimine (PEI), reduced graphene oxide (rGO), and gold nanoclusters (AuNCs), and the treated SPEs showed excellent electrical conductivity. The modified SPEs were then characterized with UV-Vis, SEM, TEM, and FTIR to analyze the morphology and composition of the AuNCs and the rGO. An anti-β-Lg antibody was then immobilized on the composite material obtained by modifying rGO with PEI and AuNCs (PEI-rGO-AuNCs), leading to the remarkable reduction in conductivity of the SPEs due to the reaction between antigen and antibody. The sensor obtained using this novel approach enabled a limit of detection (LOD) of 0.08 ng/mL and a detection range from 0.01 to 100 ng/mL for β-Lg. Furthermore, pure milk samples from four milk brands were measured using electrochemical sensors, and the results were in excellent agreement with those from commercial enzyme-linked immunosorbent assay (ELISA) methods.A simple and low-cost electrochemical sensor based on multimodified screen-printed electrodes (SPEs) was successfully synthesized for the sensitive detection of β-lactoglobulin (β-Lg). The surface treatment of SPEs was accomplished by a simple drip coating method using polyethyleneimine (PEI), reduced graphene oxide (rGO), and gold nanoclusters (AuNCs), and the treated SPEs showed excellent electrical conductivity. The modified SPEs were then characterized with UV-Vis, SEM, TEM, and FTIR to analyze the morphology and composition of the AuNCs and the rGO. An anti-β-Lg antibody was then immobilized on the composite material obtained by modifying rGO with PEI and AuNCs (PEI-rGO-AuNCs), leading to the remarkable reduction in conductivity of the SPEs due to the reaction between antigen and antibody. The sensor obtained using this novel approach enabled a limit of detection (LOD) of 0.08 ng/mL and a detection range from 0.01 to 100 ng/mL for β-Lg. Furthermore, pure milk samples from four milk brands were measured using electrochemical sensors, and the results were in excellent agreement with those from commercial enzyme-linked immunosorbent assay (ELISA) methods.
Author Wang, Yuxian
Hong, Jingyi
Jiang, Ling
Zhu, Liying
AuthorAffiliation 3 School of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing 211816, China; zlyhappy@njtech.edu.cn
1 College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, China; hongjingyi1019@163.com
2 College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, China; yxwang@njtech.edu.cn
AuthorAffiliation_xml – name: 3 School of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing 211816, China; zlyhappy@njtech.edu.cn
– name: 1 College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, China; hongjingyi1019@163.com
– name: 2 College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, China; yxwang@njtech.edu.cn
Author_xml – sequence: 1
  givenname: Jingyi
  surname: Hong
  fullname: Hong, Jingyi
– sequence: 2
  givenname: Yuxian
  surname: Wang
  fullname: Wang, Yuxian
– sequence: 3
  givenname: Liying
  surname: Zhu
  fullname: Zhu, Liying
– sequence: 4
  givenname: Ling
  orcidid: 0000-0001-6625-5557
  surname: Jiang
  fullname: Jiang, Ling
BackLink https://www.ncbi.nlm.nih.gov/pubmed/32708669$$D View this record in MEDLINE/PubMed
BookMark eNptkt1qFDEUgAep2B-98AVkwBt7MTZ_k5ncCLWta2GrQvV6yCRndrNmkzXJCD6EL-OD-Exm3HVpixBIOOfLx0nOOS4OnHdQFM8xek2pQGeRIMyoqPmj4ggzwqqWEHRw53xYHMe4QohQStsnxSElDWo5F0fFz3NXXllQKXi1hLVR0pa34KIP5VsZQZfelTNvdfVBOq_sGBOE6sZrM5icnAW5WYKD8lYFAFd9CsalHN8ZNcRyyKa0hPISUo6ZrPND-ftXNZcq-YX1_WiNK_O6Mfbr0-LxIG2EZ7v9pPjy7urzxftq_nF2fXE-rxTjIlUt1w0FwYBCXwvWS8EQRVwhrSUaMGsl6H6Qg2xB1kTqhpOhhhZh0QgOpKYnxfXWq71cdZtg1jL86Lw03d-AD4tOhmSUhY42XGDci4FjyoArqWgDjGChMAghJ9ebrWsz9mvQClwK0t6T3s84s-wW_nvXMEwoa7Lg1U4Q_LcRYurWJiqwVjrwY-wIIw0RgrYT-vIBuvJjcPmrJqpmom7ERL24W9G-lH9dz8DpFlDBxxhg2CMYddNEdfuJyuzZA1aZJKdG5scY-58bfwBmY86W
CitedBy_id crossref_primary_10_1016_j_foodchem_2023_137714
crossref_primary_10_1016_j_matchemphys_2023_128786
crossref_primary_10_1016_j_foodchem_2023_137638
crossref_primary_10_1016_j_aca_2025_343626
crossref_primary_10_1016_j_ijbiomac_2025_143010
crossref_primary_10_1016_j_talanta_2024_126808
crossref_primary_10_1016_j_bios_2022_114223
crossref_primary_10_1016_j_chemosphere_2024_141269
crossref_primary_10_3390_molecules26102940
crossref_primary_10_3390_s23010046
crossref_primary_10_1021_acsanm_4c07269
crossref_primary_10_1002_slct_202402437
crossref_primary_10_3390_bios12070503
crossref_primary_10_1016_j_snb_2023_135080
crossref_primary_10_1080_00032719_2023_2187412
crossref_primary_10_1016_j_ccr_2022_214842
crossref_primary_10_3390_s21248240
crossref_primary_10_1007_s12633_022_01877_3
crossref_primary_10_1016_j_microc_2024_112564
crossref_primary_10_1016_j_scitotenv_2022_158676
crossref_primary_10_1016_j_bioelechem_2021_107955
crossref_primary_10_1016_j_tifs_2022_01_033
crossref_primary_10_1016_j_heliyon_2023_e15327
crossref_primary_10_3390_mi13101762
Cites_doi 10.1002/smll.201202896
10.1016/j.talanta.2016.03.062
10.1021/acssensors.7b00850
10.1002/smll.201302648
10.5740/jaoacint.17-0356
10.1080/09540105.2016.1183596
10.1002/smll.201501037
10.1016/j.foodchem.2012.09.002
10.1007/s12010-011-9465-y
10.1016/j.aca.2020.04.066
10.1002/bio.3557
10.1016/j.carbon.2015.07.005
10.1021/am3018652
10.1002/aenm.201801280
10.3390/nu7032015
10.1016/j.bios.2017.10.026
10.1016/j.tsf.2013.02.011
10.1021/jz9003137
10.1002/jsfa.8489
10.1016/j.talanta.2014.07.076
10.1016/j.chroma.2008.04.075
10.1016/j.bios.2018.08.051
10.1111/1541-4337.12318
10.1016/j.mechmat.2015.03.009
10.1016/j.bios.2016.07.101
10.1002/chem.201900806
10.1016/j.aca.2016.11.016
10.1016/j.jaci.2010.11.044
10.1007/s12274-019-2314-y
10.1016/j.snb.2017.06.136
10.1021/am100372m
10.1007/s00216-009-2989-0
10.1016/j.carbon.2011.06.077
10.1016/j.smallrumres.2006.09.016
10.1016/j.chroma.2015.01.081
10.1016/j.bios.2016.08.076
10.1016/j.carbon.2012.02.002
10.1016/j.bios.2012.06.008
10.1016/j.foodcont.2016.11.030
10.1016/j.talanta.2016.05.079
10.1126/sciadv.1701247
10.1039/C6AN00359A
ContentType Journal Article
Copyright 2020. This work is licensed under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
2020 by the authors. 2020
Copyright_xml – notice: 2020. This work is licensed under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
– notice: 2020 by the authors. 2020
DBID AAYXX
CITATION
CGR
CUY
CVF
ECM
EIF
NPM
3V.
7X7
7XB
88E
8FI
8FJ
8FK
ABUWG
AFKRA
AZQEC
BENPR
CCPQU
DWQXO
FYUFA
GHDGH
K9.
M0S
M1P
PHGZM
PHGZT
PIMPY
PJZUB
PKEHL
PPXIY
PQEST
PQQKQ
PQUKI
PRINS
7X8
5PM
DOA
DOI 10.3390/s20143956
DatabaseName CrossRef
Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
ProQuest Central (Corporate)
Health & Medical Collection
ProQuest Central (purchase pre-March 2016)
Medical Database (Alumni Edition)
ProQuest Hospital Collection
Hospital Premium Collection (Alumni Edition)
ProQuest Central (Alumni) (purchase pre-March 2016)
ProQuest Central (Alumni)
ProQuest Central UK/Ireland
ProQuest Central Essentials - QC
ProQuest Central
ProQuest One Community College
ProQuest Central
Health Research Premium Collection
Health Research Premium Collection (Alumni)
ProQuest Health & Medical Complete (Alumni)
ProQuest Health & Medical Collection
Medical Database
ProQuest Central Premium
ProQuest One Academic (New)
Publicly Available Content Database
ProQuest Health & Medical Research Collection
ProQuest One Academic Middle East (New)
ProQuest One Health & Nursing
ProQuest One Academic Eastern Edition (DO NOT USE)
ProQuest One Academic (retired)
ProQuest One Academic UKI Edition
ProQuest Central China
MEDLINE - Academic
PubMed Central (Full Participant titles)
DOAJ Directory of Open Access Journals
DatabaseTitle CrossRef
MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
Publicly Available Content Database
ProQuest One Academic Middle East (New)
ProQuest Central Essentials
ProQuest Health & Medical Complete (Alumni)
ProQuest Central (Alumni Edition)
ProQuest One Community College
ProQuest One Health & Nursing
ProQuest Central China
ProQuest Central
ProQuest Health & Medical Research Collection
Health Research Premium Collection
Health and Medicine Complete (Alumni Edition)
ProQuest Central Korea
Health & Medical Research Collection
ProQuest Central (New)
ProQuest Medical Library (Alumni)
ProQuest One Academic Eastern Edition
ProQuest Hospital Collection
Health Research Premium Collection (Alumni)
ProQuest Hospital Collection (Alumni)
ProQuest Health & Medical Complete
ProQuest Medical Library
ProQuest One Academic UKI Edition
ProQuest One Academic
ProQuest One Academic (New)
ProQuest Central (Alumni)
MEDLINE - Academic
DatabaseTitleList
CrossRef

MEDLINE
Publicly Available Content Database
MEDLINE - Academic
Database_xml – sequence: 1
  dbid: DOA
  name: DOAJ Directory of Open Access Journals
  url: https://www.doaj.org/
  sourceTypes: Open Website
– sequence: 2
  dbid: NPM
  name: PubMed
  url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 3
  dbid: PIMPY
  name: Publicly Available Content Database
  url: http://search.proquest.com/publiccontent
  sourceTypes: Aggregation Database
DeliveryMethod fulltext_linktorsrc
Discipline Engineering
EISSN 1424-8220
ExternalDocumentID oai_doaj_org_article_376911b9f6134e6cac37e4219c1e99a5
PMC7412347
32708669
10_3390_s20143956
Genre Journal Article
GeographicLocations United States--US
China
Japan
GeographicLocations_xml – name: China
– name: United States--US
– name: Japan
GrantInformation_xml – fundername: the National Natural Science Foundation of China
  grantid: 31922070
– fundername: the National Key R&D Program of China
  grantid: 2017YFC1600404
– fundername: the Natural Science Foundation of Jiangsu Province
  grantid: BK20180038
– fundername: the Natural Science Foundation of Jiangsu Province
  grantid: BK20171461
GroupedDBID ---
123
2WC
53G
5VS
7X7
88E
8FE
8FG
8FI
8FJ
AADQD
AAHBH
AAYXX
ABDBF
ABUWG
ACUHS
ADBBV
ADMLS
AENEX
AFFHD
AFKRA
AFZYC
ALMA_UNASSIGNED_HOLDINGS
BENPR
BPHCQ
BVXVI
CCPQU
CITATION
CS3
D1I
DU5
E3Z
EBD
ESX
F5P
FYUFA
GROUPED_DOAJ
GX1
HH5
HMCUK
HYE
KQ8
L6V
M1P
M48
MODMG
M~E
OK1
OVT
P2P
P62
PHGZM
PHGZT
PIMPY
PJZUB
PPXIY
PQQKQ
PROAC
PSQYO
RNS
RPM
TUS
UKHRP
XSB
~8M
3V.
ABJCF
ALIPV
ARAPS
CGR
CUY
CVF
ECM
EIF
HCIFZ
KB.
M7S
NPM
PDBOC
7XB
8FK
AZQEC
DWQXO
K9.
PKEHL
PQEST
PQUKI
PRINS
7X8
PUEGO
5PM
ID FETCH-LOGICAL-c469t-86d73e94e3eb594ba940306c0dda0f148aedbfafa8ea52ad762f5e8019796e253
IEDL.DBID DOA
ISICitedReferencesCount 25
ISICitedReferencesURI http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000558252800001&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
ISSN 1424-8220
IngestDate Tue Oct 14 19:01:44 EDT 2025
Tue Nov 04 01:54:28 EST 2025
Fri Sep 05 11:37:40 EDT 2025
Tue Oct 07 07:03:30 EDT 2025
Wed Feb 19 02:01:49 EST 2025
Tue Nov 18 22:31:37 EST 2025
Sat Nov 29 07:20:26 EST 2025
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 14
Keywords screen-printed electrode
electrochemical sensors
β-lactoglobulin
PEI-rGO-AuNCs
Language English
License Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c469t-86d73e94e3eb594ba940306c0dda0f148aedbfafa8ea52ad762f5e8019796e253
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
ORCID 0000-0001-6625-5557
OpenAccessLink https://doaj.org/article/376911b9f6134e6cac37e4219c1e99a5
PMID 32708669
PQID 2425495797
PQPubID 2032333
ParticipantIDs doaj_primary_oai_doaj_org_article_376911b9f6134e6cac37e4219c1e99a5
pubmedcentral_primary_oai_pubmedcentral_nih_gov_7412347
proquest_miscellaneous_2427299387
proquest_journals_2425495797
pubmed_primary_32708669
crossref_primary_10_3390_s20143956
crossref_citationtrail_10_3390_s20143956
PublicationCentury 2000
PublicationDate 20200716
PublicationDateYYYYMMDD 2020-07-16
PublicationDate_xml – month: 7
  year: 2020
  text: 20200716
  day: 16
PublicationDecade 2020
PublicationPlace Switzerland
PublicationPlace_xml – name: Switzerland
– name: Basel
PublicationTitle Sensors (Basel, Switzerland)
PublicationTitleAlternate Sensors (Basel)
PublicationYear 2020
Publisher MDPI AG
MDPI
Publisher_xml – name: MDPI AG
– name: MDPI
References Wang (ref_42) 2012; 4
Campuzano (ref_19) 2015; 131
Xu (ref_20) 2020; 1120
Wu (ref_39) 2013; 9
Sayers (ref_12) 2016; 141
Nocerino (ref_3) 2015; 7
Zhang (ref_34) 2017; 253
Eissa (ref_32) 2012; 38
Shan (ref_36) 2013; 534
Sun (ref_21) 2018; 33
Sicherer (ref_2) 2011; 127
Yu (ref_37) 2009; 1
Boitz (ref_10) 2015; 1386
Zhang (ref_29) 2015; 91
Su (ref_30) 2019; 12
Fu (ref_24) 2017; 3
Li (ref_46) 2017; 87
Agamy (ref_6) 2007; 68
He (ref_14) 2018; 98
Salavagione (ref_25) 2009; 42
Wang (ref_16) 2018; 101
Sun (ref_23) 2011; 166
Li (ref_26) 2011; 49
Villa (ref_7) 2018; 17
Bonfatti (ref_8) 2008; 1195
Ji (ref_11) 2017; 74
Cao (ref_38) 2010; 2
Ashleya (ref_17) 2017; 88
Li (ref_35) 2017; 949
Choi (ref_44) 2018; 8
Fany (ref_5) 2010; 52
Rostellato (ref_9) 2013; 136
Wu (ref_28) 2016; 154
Grote (ref_41) 2015; 11
Xia (ref_40) 2014; 10
Ashley (ref_18) 2018; 3
Cai (ref_27) 2012; 50
Paschke (ref_1) 2019; 395
Tang (ref_45) 2018; 101
Wu (ref_15) 2013; 78
Mehmeti (ref_22) 2016; 159
Tong (ref_43) 2015; 94
He (ref_13) 2016; 27
Alim (ref_31) 2018; 121
Lu (ref_33) 2019; 25
Fiocchi (ref_4) 2010; 21
References_xml – volume: 9
  start-page: 1160
  year: 2013
  ident: ref_39
  article-title: Graphene-based electrochemical sensors
  publication-title: Small
  doi: 10.1002/smll.201202896
– volume: 154
  start-page: 73
  year: 2016
  ident: ref_28
  article-title: Peroxynitrous-acid-induced chemiluminescence detection of nitrite based on Microfluidic chip
  publication-title: Talanta
  doi: 10.1016/j.talanta.2016.03.062
– volume: 3
  start-page: 418
  year: 2018
  ident: ref_18
  article-title: Synthesis of molecularly imprinted polymer nanoparticles for α-casein detection using surface plasmon resonance as a milk allergen sensor
  publication-title: ACS Sens.
  doi: 10.1021/acssensors.7b00850
– volume: 10
  start-page: 2336
  year: 2014
  ident: ref_40
  article-title: One-Pot Synthesis of Platinum Nanocubes on Reduced Graphene Oxide with Enhanced Electrocatalytic Activity
  publication-title: Small
  doi: 10.1002/smll.201302648
– volume: 101
  start-page: 1868
  year: 2018
  ident: ref_16
  article-title: Development and validation of a surface plasmon resonance biosensor for specific detection of porcine serum albumin in food
  publication-title: J. AOAC Int.
  doi: 10.5740/jaoacint.17-0356
– volume: 27
  start-page: 806
  year: 2016
  ident: ref_13
  article-title: Preparation, immunological characterization and polyclonal antibody development for recombinant epitope tandem derived from bovine β-Lg
  publication-title: Food Agric. Immunol.
  doi: 10.1080/09540105.2016.1183596
– volume: 11
  start-page: 4666
  year: 2015
  ident: ref_41
  article-title: Self-Stacked Reduced Graphene Oxide Nanosheets Coated with Cobalt–Nickel Hydroxide by One-Step Electrochemical Deposition toward Flexible Electrochromic Supercapacitors
  publication-title: Small
  doi: 10.1002/smll.201501037
– volume: 136
  start-page: 364
  year: 2013
  ident: ref_9
  article-title: Separation and quantification of water buffalo milk protein fractions and genetic variants by RP-HPLC
  publication-title: Food Chem.
  doi: 10.1016/j.foodchem.2012.09.002
– volume: 166
  start-page: 764
  year: 2011
  ident: ref_23
  article-title: A Hydrogen Peroxide Biosensor Based on Direct Electrochemistry of Hemoglobin in Palladium Nanoparticles/Graphene–Chitosan Nanocomposite Film
  publication-title: Appl. Biochem. Biotechnol.
  doi: 10.1007/s12010-011-9465-y
– volume: 1120
  start-page: 1
  year: 2020
  ident: ref_20
  article-title: Electrochemical detection of β-lactoglobulin based on a highly selective DNA aptamer and flower-like Au@ BiVO4 microspheres
  publication-title: Anal. Chim. Acta
  doi: 10.1016/j.aca.2020.04.066
– volume: 33
  start-page: 1298
  year: 2018
  ident: ref_21
  article-title: Chemiluminescence-based aptasensors for various target analytes
  publication-title: Luminescence
  doi: 10.1002/bio.3557
– volume: 94
  start-page: 590
  year: 2015
  ident: ref_43
  article-title: Achieving significantly enhanced dielectric performance of reduced graphene oxide/polymer composite by covalent modification of graphene oxide surface
  publication-title: Carbon
  doi: 10.1016/j.carbon.2015.07.005
– volume: 21
  start-page: 1
  year: 2010
  ident: ref_4
  article-title: World Allergy Organization (WAO) Special Committee on Food Allergy. Diagnosis and Rationale for Action against Cow’s Milk Allergy; the WAO DRACMA guideline
  publication-title: Allergy Immunol.
– volume: 4
  start-page: 6273
  year: 2012
  ident: ref_42
  article-title: Improved Dielectric Properties of Nanocomposites Based on Poly (vinylidene fluoride) and Poly (vinyl alcohol)-Functionalized Graphene
  publication-title: ACS Appl. Mater. Interfaces
  doi: 10.1021/am3018652
– volume: 8
  start-page: 1801280
  year: 2018
  ident: ref_44
  article-title: A porphyrin/graphene framework: A highly efficient and robust electrocatalyst for carbon dioxide reduction
  publication-title: Adv. Energy Mater.
  doi: 10.1002/aenm.201801280
– volume: 7
  start-page: 2015
  year: 2015
  ident: ref_3
  article-title: The controversial role of food allergy in infantile colic: Evidence and clinical management
  publication-title: Nutrients
  doi: 10.3390/nu7032015
– volume: 101
  start-page: 253
  year: 2018
  ident: ref_45
  article-title: A sensitive sandwich-type immunosensor for the detection of galectin-3 based on N-GNRs-Fe-MOFs@ AuNPs nanocomposites and a novel AuPt-methylene blue nanorod
  publication-title: Biosens. Bioelectron.
  doi: 10.1016/j.bios.2017.10.026
– volume: 534
  start-page: 572
  year: 2013
  ident: ref_36
  article-title: Polyethyleneimine-functionalized graphene and its layer-by-layer assembly with Prussian blue
  publication-title: Thin Solid Films
  doi: 10.1016/j.tsf.2013.02.011
– volume: 52
  start-page: S166
  year: 2010
  ident: ref_5
  article-title: Update on optimized purification and characterization of natural milk allergens
  publication-title: Mol. Nutr. Food Res.
– volume: 1
  start-page: 467
  year: 2009
  ident: ref_37
  article-title: Self-assembled graphene/carbon nanotube hybrid films for supercapacitors
  publication-title: J. Phys. Chem. Lett.
  doi: 10.1021/jz9003137
– volume: 98
  start-page: 519
  year: 2018
  ident: ref_14
  article-title: Development of a H2O2-sensitive quantum dots-based fluorescent sandwich ELISA for sensitive detection of bovine β-Lg by monoclonal antibody
  publication-title: Food Agric.
  doi: 10.1002/jsfa.8489
– volume: 131
  start-page: 156
  year: 2015
  ident: ref_19
  article-title: Electrochemical magnetoimmunosensing platform for determination of the milk allergen β-Lg
  publication-title: Talanta
  doi: 10.1016/j.talanta.2014.07.076
– volume: 42
  start-page: 6331
  year: 2009
  ident: ref_25
  article-title: Polymeric modification of graphene through esterification of graphite oxide and poly (vinyl alcohol)
  publication-title: Int. J. Biol. Macromol.
– volume: 1195
  start-page: 101
  year: 2008
  ident: ref_8
  article-title: Validation of a new reversed-phase high-performance liquid chromatography method for separation and quantification of bovine milk protein genetic variants
  publication-title: J. Chromatogr. A
  doi: 10.1016/j.chroma.2008.04.075
– volume: 121
  start-page: 125
  year: 2018
  ident: ref_31
  article-title: Recent uses of carbon nanotubes & gold nanoparticles in electrochemistry with application in biosensing: A review
  publication-title: Biosens. Bioelectron.
  doi: 10.1016/j.bios.2018.08.051
– volume: 17
  start-page: 137
  year: 2018
  ident: ref_7
  article-title: Bovine Milk Allergens: A Comprehensive Review
  publication-title: Compr. Rev. Food Sci. Food Saf.
  doi: 10.1111/1541-4337.12318
– volume: 91
  start-page: 382
  year: 2015
  ident: ref_29
  article-title: Strain effects on thermoelectric properties of two-dimensional materials
  publication-title: Mech. Mater.
  doi: 10.1016/j.mechmat.2015.03.009
– volume: 88
  start-page: 109
  year: 2017
  ident: ref_17
  article-title: An SPR based sensor for allergens detection
  publication-title: Biosens. Bioelectron.
  doi: 10.1016/j.bios.2016.07.101
– volume: 25
  start-page: 7023
  year: 2019
  ident: ref_33
  article-title: An Ultrasensitive Electrochemical Immunosensor for Nonylphenol Leachate from Instant Noodle Containers in Southeast Asia
  publication-title: Chem.-Eur. J.
  doi: 10.1002/chem.201900806
– volume: 949
  start-page: 23
  year: 2017
  ident: ref_35
  article-title: Magnetic polyethyleneimine functionalized reduced graphene oxide as a novel magnetic solid-phase extraction adsorbent for the determination of polar acidic herbicides in rice
  publication-title: Anal. Chim. Acta
  doi: 10.1016/j.aca.2016.11.016
– volume: 127
  start-page: 594
  year: 2011
  ident: ref_2
  article-title: Epidemiology of food allergy
  publication-title: J. Allergy Clin. Immunol.
  doi: 10.1016/j.jaci.2010.11.044
– volume: 12
  start-page: 1251
  year: 2019
  ident: ref_30
  article-title: Luminescent metal nanoclusters for biomedical applications
  publication-title: Nano Res.
  doi: 10.1007/s12274-019-2314-y
– volume: 253
  start-page: 196
  year: 2017
  ident: ref_34
  article-title: Simple and sensitive fluorescence assay for acetylcholinesterase activity detection and inhibitor screening based on glutathione-capped gold nanoclusters
  publication-title: Sens. Actuator B-Chem.
  doi: 10.1016/j.snb.2017.06.136
– volume: 2
  start-page: 2339
  year: 2010
  ident: ref_38
  article-title: In situ controllable growth of prussian blue nanocubes on reduced graphene oxide: Facile synthesis and their application as enhanced nanoelectrocatalyst for H2O2 reduction
  publication-title: ACS Appl. Mater. Interfaces
  doi: 10.1021/am100372m
– volume: 395
  start-page: 15
  year: 2019
  ident: ref_1
  article-title: Allergens in foods
  publication-title: Anal. Bioanal. Chem.
  doi: 10.1007/s00216-009-2989-0
– volume: 78
  start-page: 452
  year: 2013
  ident: ref_15
  article-title: A simple and fast detection method for bovine milk residues in foods: A 2-site monoclonal antibody immunochromatography assay
  publication-title: Food Sci.
– volume: 49
  start-page: 4724
  year: 2011
  ident: ref_26
  article-title: Simultaneous surface functionalization and reduction of graphene oxide with Ctadecylamine for electrically conductive polystyrene composites
  publication-title: Carbon
  doi: 10.1016/j.carbon.2011.06.077
– volume: 68
  start-page: 64
  year: 2007
  ident: ref_6
  article-title: The challenge of cow milk protein allergy
  publication-title: Small Rumin. Res.
  doi: 10.1016/j.smallrumres.2006.09.016
– volume: 1386
  start-page: 98
  year: 2015
  ident: ref_10
  article-title: A novel ultra-high performance liquid chromatography method for the rapid determination of beta-lactoglobulin as heat load indicator in commercial milk samples
  publication-title: J. Chromatogr. A
  doi: 10.1016/j.chroma.2015.01.081
– volume: 87
  start-page: 752
  year: 2017
  ident: ref_46
  article-title: An ultrasensitive sandwich-type electrochemical immunosensor based on the signal amplification strategy of mesoporous core–shell Pd@Pt nanoparticles/amino group functionalized graphene nanocomposite
  publication-title: Biosens. Bioelectron.
  doi: 10.1016/j.bios.2016.08.076
– volume: 50
  start-page: 3407
  year: 2012
  ident: ref_27
  article-title: The use of polyethyleneimine-modified reduced graphene oxide as a substrate for silver nanoparticles to produce a material with lower cytotoxicity and long-term antibacterial activity
  publication-title: Carbon
  doi: 10.1016/j.carbon.2012.02.002
– volume: 38
  start-page: 308
  year: 2012
  ident: ref_32
  article-title: Electrochemical immunosensor for the milk allergen β-Lg based on electrografting of organic film on graphene modified screen-printed carbon electrode
  publication-title: Biosens. Bioelectron.
  doi: 10.1016/j.bios.2012.06.008
– volume: 74
  start-page: 79
  year: 2017
  ident: ref_11
  article-title: Development of a liquid chromatography-tandem mass spectrometry method for simultaneous detection of the main milk allergens
  publication-title: Food Control.
  doi: 10.1016/j.foodcont.2016.11.030
– volume: 159
  start-page: 34
  year: 2016
  ident: ref_22
  article-title: The use of graphene nanoribbons as efficient electrochemical sensing material for nitrite determinatio
  publication-title: Talanta
  doi: 10.1016/j.talanta.2016.05.079
– volume: 3
  start-page: e1701247
  year: 2017
  ident: ref_24
  article-title: Biosensing near the neutrality point of graphene
  publication-title: Sci. Adv.
  doi: 10.1126/sciadv.1701247
– volume: 141
  start-page: 4130
  year: 2016
  ident: ref_12
  article-title: The effect of thermal processing on the behaviour of peanut allergen peptide targets used in multiple reaction monitoring mass spectrometry experiments
  publication-title: Analyst
  doi: 10.1039/C6AN00359A
SSID ssj0023338
Score 2.4567516
Snippet A simple and low-cost electrochemical sensor based on multimodified screen-printed electrodes (SPEs) was successfully synthesized for the sensitive detection...
SourceID doaj
pubmedcentral
proquest
pubmed
crossref
SourceType Open Website
Open Access Repository
Aggregation Database
Index Database
Enrichment Source
StartPage 3956
SubjectTerms Allergens
Animals
Antibodies
Biosensing Techniques
Chromatography
Composite materials
Deoxyribonucleic acid
DNA
electrochemical sensors
Electrochemical Techniques - instrumentation
Electrodes
Food allergies
Fourier transforms
Gold
Graphene
Graphite
Lactoglobulins - analysis
Limit of Detection
Metal Nanoparticles
Methods
Milk - chemistry
PEI-rGO-AuNCs
Polymers
Proteins
screen-printed electrode
Sensors
Voltammetry
Work stations
β-lactoglobulin
SummonAdditionalLinks – databaseName: ProQuest Central
  dbid: BENPR
  link: http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV3NjtMwELagy4E98M8SWJBBHLhYmyZOHJ_QFrrLga0qFqS9RY49hooqWZKWt-BleBCeiZkkDS1acUHKKRlFlmb8zXz26BvGXoZeap8lIMZjEwmZQtsEUAhIoQizIlFGmnbYhJrNsosLPe8P3Jq-rXKDiS1Qu8rSGfkRlcaS7pTU68tvgqZG0e1qP0LjOtsjpTI5YnuT6Wz-YaBcMTKwTk8oRnJ_1EQkZ6dpWvVWFmrF-q-qMP9ulNzKPCe3_3fNd9itvubkx12Q3GXXoLzH9reUCO-zH8cln3YTcWwvIcDPkeFWNZ9gnnO8KvlptXQC0biyyzXJK4izyi08lrD8lFSvETT5uaU2HjGvSYXCbf7ooOFYG3OsNflbWLXNXyWvPP_1U7yneT-kSkId8Ryfs8Xy6wP26WT68c070U9qEBbp9UpkqVMxaAkxFImWhdGSuIgNnTOhR8ZlwBXeeJOBSSLjEIF9ApgctdIpREn8kI3KqoRHjLuwUNqqtBjbSMYILwWGTQah8i6NjJcBe7XxXG57GXOaprHMkc6Qk_PByQF7MZhedtodVxlNyP2DAcltty-q-nPe794cURiTQqE9Fj8SUmtsrEAi2NsxaG2SgB1uAiDvMaDJ_3g_YM-Hz7h76UrGlFCtWxtkNzrO0Oagi7VhJXGkkG-mOmBqJwp3lrr7pVx8aRXCsUyMYqke_3tZT9jNiE4PSCY0PWSjVb2Gp-yG_b5aNPWzfiv9BlYwLWY
  priority: 102
  providerName: ProQuest
Title An Electrochemical Sensor Based on Gold-Nanocluster-Modified Graphene Screen-Printed Electrodes for the Detection of β-Lactoglobulin in Milk
URI https://www.ncbi.nlm.nih.gov/pubmed/32708669
https://www.proquest.com/docview/2425495797
https://www.proquest.com/docview/2427299387
https://pubmed.ncbi.nlm.nih.gov/PMC7412347
https://doaj.org/article/376911b9f6134e6cac37e4219c1e99a5
Volume 20
WOSCitedRecordID wos000558252800001&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
journalDatabaseRights – providerCode: PRVAON
  databaseName: DOAJ Directory of Open Access Journals
  customDbUrl:
  eissn: 1424-8220
  dateEnd: 99991231
  omitProxy: false
  ssIdentifier: ssj0023338
  issn: 1424-8220
  databaseCode: DOA
  dateStart: 20010101
  isFulltext: true
  titleUrlDefault: https://www.doaj.org/
  providerName: Directory of Open Access Journals
– providerCode: PRVHPJ
  databaseName: ROAD: Directory of Open Access Scholarly Resources
  customDbUrl:
  eissn: 1424-8220
  dateEnd: 99991231
  omitProxy: false
  ssIdentifier: ssj0023338
  issn: 1424-8220
  databaseCode: M~E
  dateStart: 20010101
  isFulltext: true
  titleUrlDefault: https://road.issn.org
  providerName: ISSN International Centre
– providerCode: PRVPQU
  databaseName: Health & Medical Collection
  customDbUrl:
  eissn: 1424-8220
  dateEnd: 99991231
  omitProxy: false
  ssIdentifier: ssj0023338
  issn: 1424-8220
  databaseCode: 7X7
  dateStart: 20010101
  isFulltext: true
  titleUrlDefault: https://search.proquest.com/healthcomplete
  providerName: ProQuest
– providerCode: PRVPQU
  databaseName: ProQuest Central
  customDbUrl:
  eissn: 1424-8220
  dateEnd: 99991231
  omitProxy: false
  ssIdentifier: ssj0023338
  issn: 1424-8220
  databaseCode: BENPR
  dateStart: 20010101
  isFulltext: true
  titleUrlDefault: https://www.proquest.com/central
  providerName: ProQuest
– providerCode: PRVPQU
  databaseName: Publicly Available Content Database
  customDbUrl:
  eissn: 1424-8220
  dateEnd: 99991231
  omitProxy: false
  ssIdentifier: ssj0023338
  issn: 1424-8220
  databaseCode: PIMPY
  dateStart: 20010101
  isFulltext: true
  titleUrlDefault: http://search.proquest.com/publiccontent
  providerName: ProQuest
link http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV3NjtMwELZg4QAHxD-BpTKIAxdr09iJ4-MWugsSrSoWpHKK_DPWVlQJ6g9H3oCX4UF4JsZOGrVoJS5IkQ_JKHI8M575ksk3hLxKvVC-zIENhzpjooBYBGAYFGDS0uRSCx2bTcjptJzP1Wyv1VeoCWvpgduFO0EHQH80ymPcEVBYbbkEgX5mh6CUjuylmPXswFQHtTgir5ZHiCOoP1lngcZOhS7Ve9EnkvRflVn-XSC5F3HO7pI7XapIT9sp3iPXoL5Pbu8RCD4gP09rOm4b2djuz396gcC0WdERhidHm5qeN0vHcBNt7HIbWBHYpHELj5knPQ9k1bjX0Qsbqm_YbBXII9zujg7WFFNaiikifQubWLNV08bT37_Yh9CmJ5CJhEJ2isdksfz6kHw-G3968451DRaYRVS8YWXhJAclgIPJlTBaiQAhbOqcTj0CJQ3OeO11CTrPtMON0-eAMU1JVUCW80fkqG5qeEKoS41UVhZmaDPBcVcwqO0SUuldkWkvEvJ6t_CV7djHQxOMZYUoJOio6nWUkJe96LeWcuMqoVHQXi8QWLLjCbSdqrOd6l-2k5Djne6rznXXVcBgIny8lAl50V9GpwtfUnQNzTbKIChRvESZx62p9DPhmUSYWKiEyAMjOpjq4ZV6cRmJvTG7y7iQT__Hsz0jt7LwaiBwgBbH5Giz2sJzctN-3yzWqwG5LucyjuWA3BiNp7OPg-hBOE5-jPHc7P1k9uUP0Tcjkg
linkProvider Directory of Open Access Journals
linkToHtml http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V3LbtNAFB1VBQlY8H4ECgwIJDajOvbY41kg1NKnmkSVWqTu3PHMHYiI7JIHiI_gV1jwIXwT9_qRJqhi1wVSVvFVNLLvnHuP5-Ycxl4FXmqfxiC6XRMKmUA1BJALSCAP0jxWRprKbEINBunJiT5cYT_b_8LQWGWLiRVQu9LSO_J1ao0lnSmpd2dfBLlG0elqa6FRp8UBfP-GlG3ydn8Ln-_rMNzZPn6_JxpXAWGRCk5FmjgVgZYQQR5rmRstqW-2gXMm8MgODLjcG29SMHFoHKKFjwGBXCudQEguEQj5VxDHFY2QqZNzghch36vVi6JIB-uTkMTzNHljL9S8yhrgon7277HMhTq3c-t_u0O32c2mo-Yb9Ra4w1aguMtuLOgs3mM_Ngq-Xfv92EYggR8hfy_HfBOruONlwXfLkRNYa0o7mpF4hOiXbuixQee7pOmNJYEfWRpSEodj0thw7S86mHDs_Dl20nwLptVoW8FLz3__Ej1yMyLNFZr35_jpD0ef77MPl3I_HrDVoizgEeMuyJW2Ksm7NpQRgmeOmyKFQHmXhMbLDnvTZkpmG5F28goZZUjWKKmyeVJ12Mt56FmtTHJR0Cal2zyAxMSrL8rxx6zBpgxrDJa8XHts7SQk1thIgcRSZrugtYk7bK1NuKxBuEl2nm0d9mJ-GbGJDpxMAeWsikHupqMUYx7WuT1fSRQqZNOJ7jC1lPVLS12-Ugw_Vfrn2ASHkVSP_72s5-za3nG_l_X2BwdP2PWQ3pOQIGqyxlan4xk8ZVft1-lwMn5WbWLOTi97T_wBo_SKhg
linkToPdf http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V3LbtNAFB1VLUKw4P0IFBgQSGxGccZjj2eBUEuaUrWJIhWksjLjeUBEZJc8QHwEP8OSj-CbuNcvElSx6wLJK_vKGtn3dTzX5xDyNPBC-SRyrNfTnInYlUMAGXOxy4Iki6QWuhSbkKNRcnKixhvkZ_MvDI5VNjmxTNS2MPiNvIutscA9Jdn19VjEuD94efqZoYIU7rQ2chqVixy6b18Bvs1fHPThXT_jfLD35tVrVisMMAOwcMGS2MrQKeFCl0VKZFoJ7KFNYK0OPCAF7WzmtdeJ0xHXFjKHjxwkdSVV7DgqRkD634KWXECMbY0PhuN3LdwLAf1VXEZhqILunCOVnkKl7JUKWAoFnNXd_j2kuVL1Blf_5-d1jVype226UwXHdbLh8hvk8goD403yfSene5USkKmpE-gxIPtiRnehvlta5HS_mFoGVagw0yXSSrBhYSceWne6j2zfUCzoscHxJTaeIfuGbe5o3ZwCJqDQY9O-W5RDbzktPP31gx2hzhGyseCfABSO4WT66RZ5ey7P4zbZzIvc3SXUBplURsZZz3ARQlrNIFwSF0hvY6696JDnjdekpqZvRxWRaQowDh0sbR2sQ560pqcVZ8lZRrvoeq0B0oyXJ4rZh7TOWilUHyiGmfLQ9AkXG21C6QQUOdNzSumoQ7Yb50vr3DdP_3hehzxuL0PWwq0onbtiWdoAqlNhAjZ3Kj9vVxJyCTg7Vh0i1yJgbanrV_LJx5IZHdpjHgp579_LekQuQiikRwejw_vkEscPKMiUGm-TzcVs6R6QC-bLYjKfPawjmpL35x0UvwHTHZTV
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=An+Electrochemical+Sensor+Based+on+Gold-Nanocluster-Modified+Graphene+Screen-Printed+Electrodes+for+the+Detection+of+%CE%B2-Lactoglobulin+in+Milk&rft.jtitle=Sensors+%28Basel%2C+Switzerland%29&rft.au=Jingyi+Hong&rft.au=Yuxian+Wang&rft.au=Liying+Zhu&rft.au=Ling+Jiang&rft.date=2020-07-16&rft.pub=MDPI+AG&rft.eissn=1424-8220&rft.volume=20&rft.issue=14&rft.spage=3956&rft_id=info:doi/10.3390%2Fs20143956&rft.externalDBID=DOA&rft.externalDocID=oai_doaj_org_article_376911b9f6134e6cac37e4219c1e99a5
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1424-8220&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1424-8220&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1424-8220&client=summon