An Electrochemical Sensor Based on Gold-Nanocluster-Modified Graphene Screen-Printed Electrodes for the Detection of β-Lactoglobulin in Milk

A simple and low-cost electrochemical sensor based on multimodified screen-printed electrodes (SPEs) was successfully synthesized for the sensitive detection of β-lactoglobulin (β-Lg). The surface treatment of SPEs was accomplished by a simple drip coating method using polyethyleneimine (PEI), reduc...

Celý popis

Uloženo v:

Podrobná bibliografie
Vydáno v:	Sensors (Basel, Switzerland) Ročník 20; číslo 14; s. 3956
Hlavní autoři:	Hong, Jingyi, Wang, Yuxian, Zhu, Liying, Jiang, Ling
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	Switzerland MDPI AG 16.07.2020 MDPI
Témata:	Allergens Animals Antibodies Biosensing Techniques Chromatography Composite materials Deoxyribonucleic acid DNA electrochemical sensors Electrochemical Techniques - instrumentation Electrodes Food allergies Fourier transforms Gold Graphene Graphite Lactoglobulins - analysis Limit of Detection Metal Nanoparticles Methods Milk - chemistry PEI-rGO-AuNCs Polymers Proteins screen-printed electrode Sensors Voltammetry Work stations β-lactoglobulin United States > US China Japan screen-printed electrode electrochemical sensors β-lactoglobulin PEI-rGO-AuNCs
ISSN:	1424-8220, 1424-8220
On-line přístup:	Získat plný text
Tagy:	Přidat tag Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!

Abstract	A simple and low-cost electrochemical sensor based on multimodified screen-printed electrodes (SPEs) was successfully synthesized for the sensitive detection of β-lactoglobulin (β-Lg). The surface treatment of SPEs was accomplished by a simple drip coating method using polyethyleneimine (PEI), reduced graphene oxide (rGO), and gold nanoclusters (AuNCs), and the treated SPEs showed excellent electrical conductivity. The modified SPEs were then characterized with UV-Vis, SEM, TEM, and FTIR to analyze the morphology and composition of the AuNCs and the rGO. An anti-β-Lg antibody was then immobilized on the composite material obtained by modifying rGO with PEI and AuNCs (PEI-rGO-AuNCs), leading to the remarkable reduction in conductivity of the SPEs due to the reaction between antigen and antibody. The sensor obtained using this novel approach enabled a limit of detection (LOD) of 0.08 ng/mL and a detection range from 0.01 to 100 ng/mL for β-Lg. Furthermore, pure milk samples from four milk brands were measured using electrochemical sensors, and the results were in excellent agreement with those from commercial enzyme-linked immunosorbent assay (ELISA) methods.
AbstractList	A simple and low-cost electrochemical sensor based on multimodified screen-printed electrodes (SPEs) was successfully synthesized for the sensitive detection of β-lactoglobulin (β-Lg). The surface treatment of SPEs was accomplished by a simple drip coating method using polyethyleneimine (PEI), reduced graphene oxide (rGO), and gold nanoclusters (AuNCs), and the treated SPEs showed excellent electrical conductivity. The modified SPEs were then characterized with UV-Vis, SEM, TEM, and FTIR to analyze the morphology and composition of the AuNCs and the rGO. An anti-β-Lg antibody was then immobilized on the composite material obtained by modifying rGO with PEI and AuNCs (PEI-rGO-AuNCs), leading to the remarkable reduction in conductivity of the SPEs due to the reaction between antigen and antibody. The sensor obtained using this novel approach enabled a limit of detection (LOD) of 0.08 ng/mL and a detection range from 0.01 to 100 ng/mL for β-Lg. Furthermore, pure milk samples from four milk brands were measured using electrochemical sensors, and the results were in excellent agreement with those from commercial enzyme-linked immunosorbent assay (ELISA) methods. A simple and low-cost electrochemical sensor based on multimodified screen-printed electrodes (SPEs) was successfully synthesized for the sensitive detection of β-lactoglobulin (β-Lg). The surface treatment of SPEs was accomplished by a simple drip coating method using polyethyleneimine (PEI), reduced graphene oxide (rGO), and gold nanoclusters (AuNCs), and the treated SPEs showed excellent electrical conductivity. The modified SPEs were then characterized with UV-Vis, SEM, TEM, and FTIR to analyze the morphology and composition of the AuNCs and the rGO. An anti-β-Lg antibody was then immobilized on the composite material obtained by modifying rGO with PEI and AuNCs (PEI-rGO-AuNCs), leading to the remarkable reduction in conductivity of the SPEs due to the reaction between antigen and antibody. The sensor obtained using this novel approach enabled a limit of detection (LOD) of 0.08 ng/mL and a detection range from 0.01 to 100 ng/mL for β-Lg. Furthermore, pure milk samples from four milk brands were measured using electrochemical sensors, and the results were in excellent agreement with those from commercial enzyme-linked immunosorbent assay (ELISA) methods.A simple and low-cost electrochemical sensor based on multimodified screen-printed electrodes (SPEs) was successfully synthesized for the sensitive detection of β-lactoglobulin (β-Lg). The surface treatment of SPEs was accomplished by a simple drip coating method using polyethyleneimine (PEI), reduced graphene oxide (rGO), and gold nanoclusters (AuNCs), and the treated SPEs showed excellent electrical conductivity. The modified SPEs were then characterized with UV-Vis, SEM, TEM, and FTIR to analyze the morphology and composition of the AuNCs and the rGO. An anti-β-Lg antibody was then immobilized on the composite material obtained by modifying rGO with PEI and AuNCs (PEI-rGO-AuNCs), leading to the remarkable reduction in conductivity of the SPEs due to the reaction between antigen and antibody. The sensor obtained using this novel approach enabled a limit of detection (LOD) of 0.08 ng/mL and a detection range from 0.01 to 100 ng/mL for β-Lg. Furthermore, pure milk samples from four milk brands were measured using electrochemical sensors, and the results were in excellent agreement with those from commercial enzyme-linked immunosorbent assay (ELISA) methods.
Author	Wang, Yuxian Hong, Jingyi Jiang, Ling Zhu, Liying
AuthorAffiliation	3 School of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing 211816, China; zlyhappy@njtech.edu.cn 1 College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, China; hongjingyi1019@163.com 2 College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, China; yxwang@njtech.edu.cn
AuthorAffiliation_xml	– name: 3 School of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing 211816, China; zlyhappy@njtech.edu.cn – name: 1 College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, China; hongjingyi1019@163.com – name: 2 College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, China; yxwang@njtech.edu.cn
Author_xml	– sequence: 1 givenname: Jingyi surname: Hong fullname: Hong, Jingyi – sequence: 2 givenname: Yuxian surname: Wang fullname: Wang, Yuxian – sequence: 3 givenname: Liying surname: Zhu fullname: Zhu, Liying – sequence: 4 givenname: Ling orcidid: 0000-0001-6625-5557 surname: Jiang fullname: Jiang, Ling
BackLink	https://www.ncbi.nlm.nih.gov/pubmed/32708669$$D View this record in MEDLINE/PubMed
BookMark	eNptkt1qFDEUgAep2B-98AVkwBt7MTZ_k5ncCLWta2GrQvV6yCRndrNmkzXJCD6EL-OD-Exm3HVpixBIOOfLx0nOOS4OnHdQFM8xek2pQGeRIMyoqPmj4ggzwqqWEHRw53xYHMe4QohQStsnxSElDWo5F0fFz3NXXllQKXi1hLVR0pa34KIP5VsZQZfelTNvdfVBOq_sGBOE6sZrM5icnAW5WYKD8lYFAFd9CsalHN8ZNcRyyKa0hPISUo6ZrPND-ftXNZcq-YX1_WiNK_O6Mfbr0-LxIG2EZ7v9pPjy7urzxftq_nF2fXE-rxTjIlUt1w0FwYBCXwvWS8EQRVwhrSUaMGsl6H6Qg2xB1kTqhpOhhhZh0QgOpKYnxfXWq71cdZtg1jL86Lw03d-AD4tOhmSUhY42XGDci4FjyoArqWgDjGChMAghJ9ebrWsz9mvQClwK0t6T3s84s-wW_nvXMEwoa7Lg1U4Q_LcRYurWJiqwVjrwY-wIIw0RgrYT-vIBuvJjcPmrJqpmom7ERL24W9G-lH9dz8DpFlDBxxhg2CMYddNEdfuJyuzZA1aZJKdG5scY-58bfwBmY86W
CitedBy_id	crossref_primary_10_1016_j_foodchem_2023_137714 crossref_primary_10_1016_j_matchemphys_2023_128786 crossref_primary_10_1016_j_foodchem_2023_137638 crossref_primary_10_1016_j_aca_2025_343626 crossref_primary_10_1016_j_ijbiomac_2025_143010 crossref_primary_10_1016_j_talanta_2024_126808 crossref_primary_10_1016_j_bios_2022_114223 crossref_primary_10_1016_j_chemosphere_2024_141269 crossref_primary_10_3390_molecules26102940 crossref_primary_10_3390_s23010046 crossref_primary_10_1021_acsanm_4c07269 crossref_primary_10_1002_slct_202402437 crossref_primary_10_3390_bios12070503 crossref_primary_10_1016_j_snb_2023_135080 crossref_primary_10_1080_00032719_2023_2187412 crossref_primary_10_1016_j_ccr_2022_214842 crossref_primary_10_3390_s21248240 crossref_primary_10_1007_s12633_022_01877_3 crossref_primary_10_1016_j_microc_2024_112564 crossref_primary_10_1016_j_scitotenv_2022_158676 crossref_primary_10_1016_j_bioelechem_2021_107955 crossref_primary_10_1016_j_tifs_2022_01_033 crossref_primary_10_1016_j_heliyon_2023_e15327 crossref_primary_10_3390_mi13101762
Cites_doi	10.1002/smll.201202896 10.1016/j.talanta.2016.03.062 10.1021/acssensors.7b00850 10.1002/smll.201302648 10.5740/jaoacint.17-0356 10.1080/09540105.2016.1183596 10.1002/smll.201501037 10.1016/j.foodchem.2012.09.002 10.1007/s12010-011-9465-y 10.1016/j.aca.2020.04.066 10.1002/bio.3557 10.1016/j.carbon.2015.07.005 10.1021/am3018652 10.1002/aenm.201801280 10.3390/nu7032015 10.1016/j.bios.2017.10.026 10.1016/j.tsf.2013.02.011 10.1021/jz9003137 10.1002/jsfa.8489 10.1016/j.talanta.2014.07.076 10.1016/j.chroma.2008.04.075 10.1016/j.bios.2018.08.051 10.1111/1541-4337.12318 10.1016/j.mechmat.2015.03.009 10.1016/j.bios.2016.07.101 10.1002/chem.201900806 10.1016/j.aca.2016.11.016 10.1016/j.jaci.2010.11.044 10.1007/s12274-019-2314-y 10.1016/j.snb.2017.06.136 10.1021/am100372m 10.1007/s00216-009-2989-0 10.1016/j.carbon.2011.06.077 10.1016/j.smallrumres.2006.09.016 10.1016/j.chroma.2015.01.081 10.1016/j.bios.2016.08.076 10.1016/j.carbon.2012.02.002 10.1016/j.bios.2012.06.008 10.1016/j.foodcont.2016.11.030 10.1016/j.talanta.2016.05.079 10.1126/sciadv.1701247 10.1039/C6AN00359A
ContentType	Journal Article
Copyright	2020. This work is licensed under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 2020 by the authors. 2020
Copyright_xml	– notice: 2020. This work is licensed under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. – notice: 2020 by the authors. 2020
DBID	AAYXX CITATION CGR CUY CVF ECM EIF NPM 3V. 7X7 7XB 88E 8FI 8FJ 8FK ABUWG AFKRA AZQEC BENPR CCPQU DWQXO FYUFA GHDGH K9. M0S M1P PHGZM PHGZT PIMPY PJZUB PKEHL PPXIY PQEST PQQKQ PQUKI PRINS 7X8 5PM DOA
DOI	10.3390/s20143956
DatabaseName	CrossRef Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed ProQuest Central (Corporate) Health & Medical Collection ProQuest Central (purchase pre-March 2016) Medical Database (Alumni Edition) Hospital Premium Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) ProQuest Central ProQuest Central UK/Ireland ProQuest Central Essentials - QC ProQuest Central ProQuest One ProQuest Central Health Research Premium Collection Health Research Premium Collection (Alumni) ProQuest Health & Medical Complete (Alumni) ProQuest Health & Medical Collection Medical Database Proquest Central Premium ProQuest One Academic (New) Publicly Available Content Database ProQuest Health & Medical Research Collection ProQuest One Academic Middle East (New) ProQuest One Health & Nursing ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Academic (retired) ProQuest One Academic UKI Edition ProQuest Central China MEDLINE - Academic PubMed Central (Full Participant titles) DOAJ Directory of Open Access Journals
DatabaseTitle	CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) Publicly Available Content Database ProQuest One Academic Middle East (New) ProQuest Central Essentials ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) ProQuest One Community College ProQuest One Health & Nursing ProQuest Central China ProQuest Central ProQuest Health & Medical Research Collection Health Research Premium Collection Health and Medicine Complete (Alumni Edition) ProQuest Central Korea Health & Medical Research Collection ProQuest Central (New) ProQuest Medical Library (Alumni) ProQuest One Academic Eastern Edition ProQuest Hospital Collection Health Research Premium Collection (Alumni) ProQuest Hospital Collection (Alumni) ProQuest Health & Medical Complete ProQuest Medical Library ProQuest One Academic UKI Edition ProQuest One Academic ProQuest One Academic (New) ProQuest Central (Alumni) MEDLINE - Academic
DatabaseTitleList	CrossRef MEDLINE Publicly Available Content Database MEDLINE - Academic
Database_xml	– sequence: 1 dbid: DOA name: DOAJ Directory of Open Access Journals url: https://www.doaj.org/ sourceTypes: Open Website – sequence: 2 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 3 dbid: PIMPY name: Publicly Available Content Database url: http://search.proquest.com/publiccontent sourceTypes: Aggregation Database
DeliveryMethod	fulltext_linktorsrc
Discipline	Engineering
EISSN	1424-8220
ExternalDocumentID	oai_doaj_org_article_376911b9f6134e6cac37e4219c1e99a5 PMC7412347 32708669 10_3390_s20143956
Genre	Journal Article
GeographicLocations	United States--US China Japan
GeographicLocations_xml	– name: China – name: United States--US – name: Japan
GrantInformation_xml	– fundername: the National Natural Science Foundation of China grantid: 31922070 – fundername: the National Key R&D Program of China grantid: 2017YFC1600404 – fundername: the Natural Science Foundation of Jiangsu Province grantid: BK20180038 – fundername: the Natural Science Foundation of Jiangsu Province grantid: BK20171461
GroupedDBID	--- 123 2WC 53G 5VS 7X7 88E 8FE 8FG 8FI 8FJ AADQD AAHBH AAYXX ABDBF ABUWG ACUHS ADBBV ADMLS AENEX AFFHD AFKRA AFZYC ALMA_UNASSIGNED_HOLDINGS BENPR BPHCQ BVXVI CCPQU CITATION CS3 D1I DU5 E3Z EBD ESX F5P FYUFA GROUPED_DOAJ GX1 HH5 HMCUK HYE KQ8 L6V M1P M48 MODMG M~E OK1 OVT P2P P62 PHGZM PHGZT PIMPY PJZUB PPXIY PQQKQ PROAC PSQYO RNS RPM TUS UKHRP XSB ~8M 3V. ABJCF ALIPV ARAPS CGR CUY CVF ECM EIF HCIFZ KB. M7S NPM PDBOC 7XB 8FK AZQEC DWQXO K9. PKEHL PQEST PQUKI PRINS 7X8 PUEGO 5PM
ID	FETCH-LOGICAL-c469t-86d73e94e3eb594ba940306c0dda0f148aedbfafa8ea52ad762f5e8019796e253
IEDL.DBID	DOA
ISICitedReferencesCount	25
ISICitedReferencesURI	http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000558252800001&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
ISSN	1424-8220
IngestDate	Tue Oct 14 19:01:44 EDT 2025 Tue Nov 04 01:54:28 EST 2025 Fri Sep 05 11:37:40 EDT 2025 Tue Oct 07 07:03:30 EDT 2025 Wed Feb 19 02:01:49 EST 2025 Tue Nov 18 22:31:37 EST 2025 Sat Nov 29 07:20:26 EST 2025
IsDoiOpenAccess	true
IsOpenAccess	true
IsPeerReviewed	true
IsScholarly	true
Issue	14
Keywords	screen-printed electrode electrochemical sensors β-lactoglobulin PEI-rGO-AuNCs
Language	English
License	Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
LinkModel	DirectLink
MergedId	FETCHMERGED-LOGICAL-c469t-86d73e94e3eb594ba940306c0dda0f148aedbfafa8ea52ad762f5e8019796e253
Notes	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23
ORCID	0000-0001-6625-5557
OpenAccessLink	https://doaj.org/article/376911b9f6134e6cac37e4219c1e99a5
PMID	32708669
PQID	2425495797
PQPubID	2032333
ParticipantIDs	doaj_primary_oai_doaj_org_article_376911b9f6134e6cac37e4219c1e99a5 pubmedcentral_primary_oai_pubmedcentral_nih_gov_7412347 proquest_miscellaneous_2427299387 proquest_journals_2425495797 pubmed_primary_32708669 crossref_primary_10_3390_s20143956 crossref_citationtrail_10_3390_s20143956
PublicationCentury	2000
PublicationDate	20200716
PublicationDateYYYYMMDD	2020-07-16
PublicationDate_xml	– month: 7 year: 2020 text: 20200716 day: 16
PublicationDecade	2020
PublicationPlace	Switzerland
PublicationPlace_xml	– name: Switzerland – name: Basel
PublicationTitle	Sensors (Basel, Switzerland)
PublicationTitleAlternate	Sensors (Basel)
PublicationYear	2020
Publisher	MDPI AG MDPI
Publisher_xml	– name: MDPI AG – name: MDPI
References	Wang (ref_42) 2012; 4 Campuzano (ref_19) 2015; 131 Xu (ref_20) 2020; 1120 Wu (ref_39) 2013; 9 Sayers (ref_12) 2016; 141 Nocerino (ref_3) 2015; 7 Zhang (ref_34) 2017; 253 Eissa (ref_32) 2012; 38 Shan (ref_36) 2013; 534 Sun (ref_21) 2018; 33 Sicherer (ref_2) 2011; 127 Yu (ref_37) 2009; 1 Boitz (ref_10) 2015; 1386 Zhang (ref_29) 2015; 91 Su (ref_30) 2019; 12 Fu (ref_24) 2017; 3 Li (ref_46) 2017; 87 Agamy (ref_6) 2007; 68 He (ref_14) 2018; 98 Salavagione (ref_25) 2009; 42 Wang (ref_16) 2018; 101 Sun (ref_23) 2011; 166 Li (ref_26) 2011; 49 Villa (ref_7) 2018; 17 Bonfatti (ref_8) 2008; 1195 Ji (ref_11) 2017; 74 Cao (ref_38) 2010; 2 Ashleya (ref_17) 2017; 88 Li (ref_35) 2017; 949 Choi (ref_44) 2018; 8 Fany (ref_5) 2010; 52 Rostellato (ref_9) 2013; 136 Wu (ref_28) 2016; 154 Grote (ref_41) 2015; 11 Xia (ref_40) 2014; 10 Ashley (ref_18) 2018; 3 Cai (ref_27) 2012; 50 Paschke (ref_1) 2019; 395 Tang (ref_45) 2018; 101 Wu (ref_15) 2013; 78 Mehmeti (ref_22) 2016; 159 Tong (ref_43) 2015; 94 He (ref_13) 2016; 27 Alim (ref_31) 2018; 121 Lu (ref_33) 2019; 25 Fiocchi (ref_4) 2010; 21
References_xml	– volume: 9 start-page: 1160 year: 2013 ident: ref_39 article-title: Graphene-based electrochemical sensors publication-title: Small doi: 10.1002/smll.201202896 – volume: 154 start-page: 73 year: 2016 ident: ref_28 article-title: Peroxynitrous-acid-induced chemiluminescence detection of nitrite based on Microfluidic chip publication-title: Talanta doi: 10.1016/j.talanta.2016.03.062 – volume: 3 start-page: 418 year: 2018 ident: ref_18 article-title: Synthesis of molecularly imprinted polymer nanoparticles for α-casein detection using surface plasmon resonance as a milk allergen sensor publication-title: ACS Sens. doi: 10.1021/acssensors.7b00850 – volume: 10 start-page: 2336 year: 2014 ident: ref_40 article-title: One-Pot Synthesis of Platinum Nanocubes on Reduced Graphene Oxide with Enhanced Electrocatalytic Activity publication-title: Small doi: 10.1002/smll.201302648 – volume: 101 start-page: 1868 year: 2018 ident: ref_16 article-title: Development and validation of a surface plasmon resonance biosensor for specific detection of porcine serum albumin in food publication-title: J. AOAC Int. doi: 10.5740/jaoacint.17-0356 – volume: 27 start-page: 806 year: 2016 ident: ref_13 article-title: Preparation, immunological characterization and polyclonal antibody development for recombinant epitope tandem derived from bovine β-Lg publication-title: Food Agric. Immunol. doi: 10.1080/09540105.2016.1183596 – volume: 11 start-page: 4666 year: 2015 ident: ref_41 article-title: Self-Stacked Reduced Graphene Oxide Nanosheets Coated with Cobalt–Nickel Hydroxide by One-Step Electrochemical Deposition toward Flexible Electrochromic Supercapacitors publication-title: Small doi: 10.1002/smll.201501037 – volume: 136 start-page: 364 year: 2013 ident: ref_9 article-title: Separation and quantification of water buffalo milk protein fractions and genetic variants by RP-HPLC publication-title: Food Chem. doi: 10.1016/j.foodchem.2012.09.002 – volume: 166 start-page: 764 year: 2011 ident: ref_23 article-title: A Hydrogen Peroxide Biosensor Based on Direct Electrochemistry of Hemoglobin in Palladium Nanoparticles/Graphene–Chitosan Nanocomposite Film publication-title: Appl. Biochem. Biotechnol. doi: 10.1007/s12010-011-9465-y – volume: 1120 start-page: 1 year: 2020 ident: ref_20 article-title: Electrochemical detection of β-lactoglobulin based on a highly selective DNA aptamer and flower-like Au@ BiVO4 microspheres publication-title: Anal. Chim. Acta doi: 10.1016/j.aca.2020.04.066 – volume: 33 start-page: 1298 year: 2018 ident: ref_21 article-title: Chemiluminescence-based aptasensors for various target analytes publication-title: Luminescence doi: 10.1002/bio.3557 – volume: 94 start-page: 590 year: 2015 ident: ref_43 article-title: Achieving significantly enhanced dielectric performance of reduced graphene oxide/polymer composite by covalent modification of graphene oxide surface publication-title: Carbon doi: 10.1016/j.carbon.2015.07.005 – volume: 21 start-page: 1 year: 2010 ident: ref_4 article-title: World Allergy Organization (WAO) Special Committee on Food Allergy. Diagnosis and Rationale for Action against Cow’s Milk Allergy; the WAO DRACMA guideline publication-title: Allergy Immunol. – volume: 4 start-page: 6273 year: 2012 ident: ref_42 article-title: Improved Dielectric Properties of Nanocomposites Based on Poly (vinylidene fluoride) and Poly (vinyl alcohol)-Functionalized Graphene publication-title: ACS Appl. Mater. Interfaces doi: 10.1021/am3018652 – volume: 8 start-page: 1801280 year: 2018 ident: ref_44 article-title: A porphyrin/graphene framework: A highly efficient and robust electrocatalyst for carbon dioxide reduction publication-title: Adv. Energy Mater. doi: 10.1002/aenm.201801280 – volume: 7 start-page: 2015 year: 2015 ident: ref_3 article-title: The controversial role of food allergy in infantile colic: Evidence and clinical management publication-title: Nutrients doi: 10.3390/nu7032015 – volume: 101 start-page: 253 year: 2018 ident: ref_45 article-title: A sensitive sandwich-type immunosensor for the detection of galectin-3 based on N-GNRs-Fe-MOFs@ AuNPs nanocomposites and a novel AuPt-methylene blue nanorod publication-title: Biosens. Bioelectron. doi: 10.1016/j.bios.2017.10.026 – volume: 534 start-page: 572 year: 2013 ident: ref_36 article-title: Polyethyleneimine-functionalized graphene and its layer-by-layer assembly with Prussian blue publication-title: Thin Solid Films doi: 10.1016/j.tsf.2013.02.011 – volume: 52 start-page: S166 year: 2010 ident: ref_5 article-title: Update on optimized purification and characterization of natural milk allergens publication-title: Mol. Nutr. Food Res. – volume: 1 start-page: 467 year: 2009 ident: ref_37 article-title: Self-assembled graphene/carbon nanotube hybrid films for supercapacitors publication-title: J. Phys. Chem. Lett. doi: 10.1021/jz9003137 – volume: 98 start-page: 519 year: 2018 ident: ref_14 article-title: Development of a H2O2-sensitive quantum dots-based fluorescent sandwich ELISA for sensitive detection of bovine β-Lg by monoclonal antibody publication-title: Food Agric. doi: 10.1002/jsfa.8489 – volume: 131 start-page: 156 year: 2015 ident: ref_19 article-title: Electrochemical magnetoimmunosensing platform for determination of the milk allergen β-Lg publication-title: Talanta doi: 10.1016/j.talanta.2014.07.076 – volume: 42 start-page: 6331 year: 2009 ident: ref_25 article-title: Polymeric modification of graphene through esterification of graphite oxide and poly (vinyl alcohol) publication-title: Int. J. Biol. Macromol. – volume: 1195 start-page: 101 year: 2008 ident: ref_8 article-title: Validation of a new reversed-phase high-performance liquid chromatography method for separation and quantification of bovine milk protein genetic variants publication-title: J. Chromatogr. A doi: 10.1016/j.chroma.2008.04.075 – volume: 121 start-page: 125 year: 2018 ident: ref_31 article-title: Recent uses of carbon nanotubes & gold nanoparticles in electrochemistry with application in biosensing: A review publication-title: Biosens. Bioelectron. doi: 10.1016/j.bios.2018.08.051 – volume: 17 start-page: 137 year: 2018 ident: ref_7 article-title: Bovine Milk Allergens: A Comprehensive Review publication-title: Compr. Rev. Food Sci. Food Saf. doi: 10.1111/1541-4337.12318 – volume: 91 start-page: 382 year: 2015 ident: ref_29 article-title: Strain effects on thermoelectric properties of two-dimensional materials publication-title: Mech. Mater. doi: 10.1016/j.mechmat.2015.03.009 – volume: 88 start-page: 109 year: 2017 ident: ref_17 article-title: An SPR based sensor for allergens detection publication-title: Biosens. Bioelectron. doi: 10.1016/j.bios.2016.07.101 – volume: 25 start-page: 7023 year: 2019 ident: ref_33 article-title: An Ultrasensitive Electrochemical Immunosensor for Nonylphenol Leachate from Instant Noodle Containers in Southeast Asia publication-title: Chem.-Eur. J. doi: 10.1002/chem.201900806 – volume: 949 start-page: 23 year: 2017 ident: ref_35 article-title: Magnetic polyethyleneimine functionalized reduced graphene oxide as a novel magnetic solid-phase extraction adsorbent for the determination of polar acidic herbicides in rice publication-title: Anal. Chim. Acta doi: 10.1016/j.aca.2016.11.016 – volume: 127 start-page: 594 year: 2011 ident: ref_2 article-title: Epidemiology of food allergy publication-title: J. Allergy Clin. Immunol. doi: 10.1016/j.jaci.2010.11.044 – volume: 12 start-page: 1251 year: 2019 ident: ref_30 article-title: Luminescent metal nanoclusters for biomedical applications publication-title: Nano Res. doi: 10.1007/s12274-019-2314-y – volume: 253 start-page: 196 year: 2017 ident: ref_34 article-title: Simple and sensitive fluorescence assay for acetylcholinesterase activity detection and inhibitor screening based on glutathione-capped gold nanoclusters publication-title: Sens. Actuator B-Chem. doi: 10.1016/j.snb.2017.06.136 – volume: 2 start-page: 2339 year: 2010 ident: ref_38 article-title: In situ controllable growth of prussian blue nanocubes on reduced graphene oxide: Facile synthesis and their application as enhanced nanoelectrocatalyst for H2O2 reduction publication-title: ACS Appl. Mater. Interfaces doi: 10.1021/am100372m – volume: 395 start-page: 15 year: 2019 ident: ref_1 article-title: Allergens in foods publication-title: Anal. Bioanal. Chem. doi: 10.1007/s00216-009-2989-0 – volume: 78 start-page: 452 year: 2013 ident: ref_15 article-title: A simple and fast detection method for bovine milk residues in foods: A 2-site monoclonal antibody immunochromatography assay publication-title: Food Sci. – volume: 49 start-page: 4724 year: 2011 ident: ref_26 article-title: Simultaneous surface functionalization and reduction of graphene oxide with Ctadecylamine for electrically conductive polystyrene composites publication-title: Carbon doi: 10.1016/j.carbon.2011.06.077 – volume: 68 start-page: 64 year: 2007 ident: ref_6 article-title: The challenge of cow milk protein allergy publication-title: Small Rumin. Res. doi: 10.1016/j.smallrumres.2006.09.016 – volume: 1386 start-page: 98 year: 2015 ident: ref_10 article-title: A novel ultra-high performance liquid chromatography method for the rapid determination of beta-lactoglobulin as heat load indicator in commercial milk samples publication-title: J. Chromatogr. A doi: 10.1016/j.chroma.2015.01.081 – volume: 87 start-page: 752 year: 2017 ident: ref_46 article-title: An ultrasensitive sandwich-type electrochemical immunosensor based on the signal amplification strategy of mesoporous core–shell Pd@Pt nanoparticles/amino group functionalized graphene nanocomposite publication-title: Biosens. Bioelectron. doi: 10.1016/j.bios.2016.08.076 – volume: 50 start-page: 3407 year: 2012 ident: ref_27 article-title: The use of polyethyleneimine-modified reduced graphene oxide as a substrate for silver nanoparticles to produce a material with lower cytotoxicity and long-term antibacterial activity publication-title: Carbon doi: 10.1016/j.carbon.2012.02.002 – volume: 38 start-page: 308 year: 2012 ident: ref_32 article-title: Electrochemical immunosensor for the milk allergen β-Lg based on electrografting of organic film on graphene modified screen-printed carbon electrode publication-title: Biosens. Bioelectron. doi: 10.1016/j.bios.2012.06.008 – volume: 74 start-page: 79 year: 2017 ident: ref_11 article-title: Development of a liquid chromatography-tandem mass spectrometry method for simultaneous detection of the main milk allergens publication-title: Food Control. doi: 10.1016/j.foodcont.2016.11.030 – volume: 159 start-page: 34 year: 2016 ident: ref_22 article-title: The use of graphene nanoribbons as efficient electrochemical sensing material for nitrite determinatio publication-title: Talanta doi: 10.1016/j.talanta.2016.05.079 – volume: 3 start-page: e1701247 year: 2017 ident: ref_24 article-title: Biosensing near the neutrality point of graphene publication-title: Sci. Adv. doi: 10.1126/sciadv.1701247 – volume: 141 start-page: 4130 year: 2016 ident: ref_12 article-title: The effect of thermal processing on the behaviour of peanut allergen peptide targets used in multiple reaction monitoring mass spectrometry experiments publication-title: Analyst doi: 10.1039/C6AN00359A
SSID	ssj0023338
Score	2.4567516
Snippet	A simple and low-cost electrochemical sensor based on multimodified screen-printed electrodes (SPEs) was successfully synthesized for the sensitive detection...
SourceID	doaj pubmedcentral proquest pubmed crossref
SourceType	Open Website Open Access Repository Aggregation Database Index Database Enrichment Source
StartPage	3956
SubjectTerms	Allergens Animals Antibodies Biosensing Techniques Chromatography Composite materials Deoxyribonucleic acid DNA electrochemical sensors Electrochemical Techniques - instrumentation Electrodes Food allergies Fourier transforms Gold Graphene Graphite Lactoglobulins - analysis Limit of Detection Metal Nanoparticles Methods Milk - chemistry PEI-rGO-AuNCs Polymers Proteins screen-printed electrode Sensors Voltammetry Work stations β-lactoglobulin
SummonAdditionalLinks	– databaseName: ProQuest Central dbid: BENPR link: http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV3NjtMwELagywEO_P8EFmQQBy7WZhPHjk9oC93lwFYVC9LeIsc_UFHFS9LyFrwMD8IzMZO6oUUrLki9pBlFlmb8zXz26BtCXma-Tp2XiskyLRnXBnDQ1PAonebC2jq1aT9sQk6n5fm5msUDty62VW4wsQdqGwyekR9gaczxTkm-vvjGcGoU3q7GERpXyR4qlfER2RtPprMPA-XKgYGt9YRyIPcHXYZydgqnVW9loV6s_7IK8-9Gya3Mc3zrf9d8m9yMNSc9WgfJHXLFNXfJjS0lwnvkx1FDJ-uJOCZKCNAzYLihpWPIc5aGhp6EhWWAxsEsViivwE6DnXsoYekJql4DaNIzg208bNaiCoXdfNG6jkJtTKHWpG_dsm_-amjw9NdP9h7n_aAqCXbEU_idzhdf75NPx5OPb96xOKmBGaDXS1YKK3OnuMtdXShea8WRi5jUWp16YFza2dprr0uni0xbQGBfOEiOSirhsiJ_QEZNaNwjQkXpDrkUBXem4MLXdSa0MWVqpOcQQGlCXm08V5koY47TNBYV0Bl0cjU4OSEvBtOLtXbHZUZjdP9ggHLb_R-h_VzF3VsBCkNSqJWH4oc7YbTJpeMA9ubQKaWLhOxvAqCKGNBVf7yfkOfDa9i9eCWjGxdWvQ2wG5WXYPNwHWvDSvJMAt8UKiFyJwp3lrr7ppl_6RXCoUzMci4f_3tZT8j1DE8PUCZU7JPRsl25p-Sa-b6cd-2zuJV-A70aLao priority: 102 providerName: ProQuest
Title	An Electrochemical Sensor Based on Gold-Nanocluster-Modified Graphene Screen-Printed Electrodes for the Detection of β-Lactoglobulin in Milk
URI	https://www.ncbi.nlm.nih.gov/pubmed/32708669 https://www.proquest.com/docview/2425495797 https://www.proquest.com/docview/2427299387 https://pubmed.ncbi.nlm.nih.gov/PMC7412347 https://doaj.org/article/376911b9f6134e6cac37e4219c1e99a5
Volume	20
WOSCitedRecordID	wos000558252800001&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
hasFullText	1
inHoldings	1
isFullTextHit
isPrint
journalDatabaseRights	– providerCode: PRVAON databaseName: DOAJ Directory of Open Access Journals customDbUrl: eissn: 1424-8220 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0023338 issn: 1424-8220 databaseCode: DOA dateStart: 20010101 isFulltext: true titleUrlDefault: https://www.doaj.org/ providerName: Directory of Open Access Journals – providerCode: PRVHPJ databaseName: ROAD: Directory of Open Access Scholarly Resources customDbUrl: eissn: 1424-8220 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0023338 issn: 1424-8220 databaseCode: M~E dateStart: 20010101 isFulltext: true titleUrlDefault: https://road.issn.org providerName: ISSN International Centre – providerCode: PRVPQU databaseName: Health & Medical Collection customDbUrl: eissn: 1424-8220 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0023338 issn: 1424-8220 databaseCode: 7X7 dateStart: 20010101 isFulltext: true titleUrlDefault: https://search.proquest.com/healthcomplete providerName: ProQuest – providerCode: PRVPQU databaseName: ProQuest Central customDbUrl: eissn: 1424-8220 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0023338 issn: 1424-8220 databaseCode: BENPR dateStart: 20010101 isFulltext: true titleUrlDefault: https://www.proquest.com/central providerName: ProQuest – providerCode: PRVPQU databaseName: Publicly Available Content Database customDbUrl: eissn: 1424-8220 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0023338 issn: 1424-8220 databaseCode: PIMPY dateStart: 20010101 isFulltext: true titleUrlDefault: http://search.proquest.com/publiccontent providerName: ProQuest
link	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV3NbtQwELagcIADKv-BdmUQBy5R08Sx42O33RYkdhVRkJZT5NhjdcUqqfanR96Al-FBeCZmkmy0iypxQYoixRlFTmbGM18y-Yaxd7EvI_BKhyqLslAYi-ugLfFQgRHSuTJyUdNsQk0m2XSq861WX1QT1tIDtw_uCB0A_bHUHuOOAGmNTRQI9DN7DFqbhr0Us54NmOqgVoLIq-URShDUHy1jorHT1KV6K_o0JP23ZZZ_F0huRZzzffaoSxX5STvFx-wOVE_Ywy0Cwafs50nFR20jG9v9-c8vEZjWCz7E8OR4XfGLeu5CXERrO18TK0I4rt3MY-bJL4isGtc6fmmp-ibMF0Qe4TZXdLDkmNJyTBH5Gayamq2K157__hV-ojY9RCZChewct_Fs_v0Z-3o--nL6IewaLIQWUfEqzKRTCWgBCZSpFqXRgiCEjZwzkUegZMCV3niTgUlj43Dh9ClgTNNKS4jT5Dnbq-oKXjIuMzgWSqYCbCqkL8tYGmuzyCovUO9RwN5vHnxhO_ZxaoIxLxCFkI6KXkcBe9uLXreUG7cJDUl7vQCxZDcDaDtFZzvFv2wnYAcb3Red6y4LwmCCPl6qgL3pT6PT0ZcUU0G9bmQQlOgkQ5kXran0M0lihTBR6oCpHSPamerumWp21RB7Y3YXJ0K9-h_39po9iOnVAHGAygO2t1qs4ZDdtzer2XIxYHfVVDX7bMDuDUeT_POg8SDcj3-McCz_OM6__QEp_SPW
linkProvider	Directory of Open Access Journals
linkToHtml	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V3LbtNAFB1VBQlY8H4ECgwIJDZWXXvs8SwQaulTTaJKLVJ27njmDkREdokTEB_Br7DgQ_gm7vWrCarYdYGUTeKraOTcOfcez805jL0KXOaDk8qTiZ94QhvEQZPhWwlaxNZmvvUrswk5HCajkTpaYT_b_8LQWGWLiRVQ28LQM_J1ao0FnSnJd2dfPHKNotPV1kKjTotD-P4NKVv59mAbf9_XQbC7c_J-32tcBTyDVHDmJbGVISgBIWSREplWgvpm41urfYfsQIPNnHY6AR0F2iJauAgQyJVUMQTkEoGQfwVxXNIImRydE7wQ-V6tXhSGyl8vAxLPU-SNvVDzKmuAi_rZv8cyF-rc7q3_7Q7dZjebjppv1lvgDluB_C67saCzeI_92Mz5Tu33YxqBBH6M_L2Y8i2s4pYXOd8rJtbDWlOYyZzEI7xBYccOG3S-R5reWBL4saEhJe9oShobtv1GCyXHzp9jJ823YVaNtuW8cPz3L69PbkakuULz_hxfg_Hk83324VLuxwO2mhc5PGI8TmBDyDgSYCIRuywLYm1M4hvpBG4Pv8fetJmSmkaknbxCJimSNUqqtEuqHnvZhZ7VyiQXBW1RunUBJCZefVBMP6YNNqVYY7DkZcphaycgNtqEEgSWMrMBSumox9bahEsbhCvT82zrsRfdZcQmOnDSORTzKga5mwoTjHlY53a3kjCQyKZj1WNyKeuXlrp8JR9_qvTPsQkOQiEf_3tZz9m1_ZNBP-0fDA-fsOsBPSchQdR4ja3OpnN4yq6ar7NxOX1WbWLOTi97T_wBi5uKyg
linkToPdf	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V3LbtNAFB1VLUKw4P0IFBgQSGysuPbY41kg1JKmRG2iSAWprMx45g5ERHbJA8RH8DMs-Qi-iXv9IkEVuy6QsnF8ZY3s-zqe63MYexq4zAcnlScTP_GENpgHTYaHErSIrc1865diE3I0Sk5O1HiD_Wy-haGxyiYnlonaFobekXepNRa0pyS7rh6LGPf6L08_e6QgRTutjZxG5SKH8O0rwrf5i0EPn_WzIOjvv3n12qsVBjyDsHDhJbGVISgBIWSREplWgnpo41urfYdIQYPNnHY6AR0F2mLmcBFgUldSxRCQYgSm_y1syQXG2NZ4MBy_a-FeiOiv4jIKQ-V35wFR6SlSyl6pgKVQwFnd7d9DmitVr3_1f75f19iVutfmu1VwXGcbkN9gl1cYGG-y77s536-UgExNncCPEdkXM76H9d3yIucHxdR6WIUKM10SrYQ3LOzEYevOD4jtG4sFPzY0vuSNZ8S-YZsrWphzxAQce2zeg0U59JbzwvFfP7wj0jkiNhb6EoDjbziZfrrF3p7L_bjNNvMih7uMxwnsCBlHAkwkYpdlQayNSXwjncDA8TvseeM1qanp20lFZJoijCMHS1sH67AnrelpxVlyltEeuV5rQDTj5R_F7ENaZ60Uqw8Ww0w5bPoExEabUILAImd2QCkdddh243xpnfvm6R_P67DH7WnMWrQVpXMolqUNojoVJmhzp_LzdiVhIBFnx6rD5FoErC11_Uw--Vgyo2N7HIRC3vv3sh6xixgK6dFgdHifXQroBQoxpcbbbHMxW8IDdsF8WUzms4d1RHP2_ryD4jfGtpUZ
openUrl	ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=An+Electrochemical+Sensor+Based+on+Gold-Nanocluster-Modified+Graphene+Screen-Printed+Electrodes+for+the+Detection+of+%CE%B2-Lactoglobulin+in+Milk&rft.jtitle=Sensors+%28Basel%2C+Switzerland%29&rft.au=Hong%2C+Jingyi&rft.au=Wang%2C+Yuxian&rft.au=Zhu%2C+Liying&rft.au=Jiang%2C+Ling&rft.date=2020-07-16&rft.issn=1424-8220&rft.eissn=1424-8220&rft.volume=20&rft.issue=14&rft.spage=3956&rft_id=info:doi/10.3390%2Fs20143956&rft.externalDBID=n%2Fa&rft.externalDocID=10_3390_s20143956
thumbnail_l	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1424-8220&client=summon
thumbnail_m	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1424-8220&client=summon
thumbnail_s	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1424-8220&client=summon