TP53 mutation hits energy metabolism and increases glycolysis in breast cancer

Promising new hallmarks of cancer is alteration of energy metabolism that involves molecular mechanisms shifting cancer cells to aerobe glycolysis. Our goal was to evaluate the correlation between mutation in the commonly mutated tumor suppressor gene TP53 and metabolism. We established a database c...

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Veröffentlicht in:Oncotarget Jg. 7; H. 41; S. 67183
Hauptverfasser: Harami-Papp, Hajnalka, Pongor, Lőrinc S, Munkácsy, Gyöngyi, Horváth, Gergő, Nagy, Ádám M, Ambrus, Attila, Hauser, Péter, Szabó, András, Tretter, László, Győrffy, Balázs
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States 11.10.2016
Schlagworte:
Breast Neoplasms - genetics
Breast Neoplasms - metabolism
Cell Line, Tumor
Energy Metabolism - genetics
Female
Humans
Mutation
Tumor Suppressor Protein p53 - genetics
breast cancer
glycolytic efficiency
next generation sequencing
Warburg effect
ISSN:1949-2553, 1949-2553
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Zusammenfassung:Promising new hallmarks of cancer is alteration of energy metabolism that involves molecular mechanisms shifting cancer cells to aerobe glycolysis. Our goal was to evaluate the correlation between mutation in the commonly mutated tumor suppressor gene TP53 and metabolism. We established a database comprising mutation and RNA-seq expression data of the TCGA repository and performed receiver operating characteristics (ROC) analysis to compare expression of each gene between TP53 mutated and wild type samples. All together 762 breast cancer samples were evaluated of which 215 had TP53 mutation. Top up-regulated metabolic genes include glycolytic enzymes (e.g. HK3, GPI, GAPDH, PGK1, ENO1), glycolysis regulator (PDK1) and pentose phosphate pathway enzymes (PGD, TKT, RPIA). Gluconeogenesis enzymes (G6PC3, FBP1) were down-regulated. Oxygen consumption and extracellular acidification rates were measured in TP53 wild type and mutant breast cell lines with a microfluorimetric analyzer. Applying metabolic inhibitors in the presence and absence of D-glucose and L-glutamine in cell culture experiments resulted in higher glycolytic and mitochondrial activity in TP53 mutant breast cancer cell lines. In summary, TP53 mutation influences energy metabolism at multiple levels. Our results provide evidence for the synergistic activation of multiple hallmarks linking to these the mutation status of a key driver gene.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:1949-2553
1949-2553
DOI:10.18632/oncotarget.11594