Effect of oral bacteria on peripheral blood leukocyte interleukin-6 and soluble interleukin-6 receptor production

To determine the effect of pathogenic oral bacteria on interleukin 6 (IL-6) and soluble IL-6 receptor production, we measured their release by human peripheral blood mononuclear cells in vitro. Unseparated peripheral blood mononuclear cells, peripheral blood lymphocytes (monocyte depleted), pure T c...

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Veröffentlicht in:Oral microbiology and immunology Jg. 11; H. 5; S. 332
Hauptverfasser: Lindemann, R A, Kinder Haake, S A, Kjeldsen, M, Avanessian, A B
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Denmark 01.10.1996
Schlagworte:
Aggregatibacter actinomycetemcomitans - immunology
Animals
Capnocytophaga - immunology
Fusobacterium nucleatum - immunology
Humans
Interleukin-6 - biosynthesis
Lymphocytes - metabolism
Macaca fascicularis
Monocytes - metabolism
Periodontitis - immunology
Periodontitis - microbiology
Porphyromonas gingivalis - immunology
Receptors, Interleukin - biosynthesis
ISSN:0902-0055
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Zusammenfassung:To determine the effect of pathogenic oral bacteria on interleukin 6 (IL-6) and soluble IL-6 receptor production, we measured their release by human peripheral blood mononuclear cells in vitro. Unseparated peripheral blood mononuclear cells, peripheral blood lymphocytes (monocyte depleted), pure T cells, or monocytes were cultured with Actinobacillus actinomycetemcomitans, Capnocytophaga gingivalis, Capnocytophaga ochracea, Fusobacterium nucleatum or Porphyromonas gingivalis for 24 h. Supernatants were tested for IL-6 and soluble IL-6 receptor by enzyme-linked immunosorbent assay. Only monocytes and peripheral blood mononuclear cells responded with significant IL-6 release in the presence of all bacteria tested. However, peripheral blood lymphocytes were capable of producing IL-6 when activated by phytohemagglutinin or IL-2 followed by bacteria, though substantially less than cultures containing monocytes. No bacteria tested increased soluble IL-6 receptor release over spontaneous soluble IL-6 receptor release. We conclude that monocytes release IL-6 after contact with oral pathogens; however, soluble IL-6 receptor from T cells and monocytes is constitutively produced and may modulate IL-6 actions.
Bibliographie:ObjectType-Article-1
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ISSN:0902-0055
DOI:10.1111/j.1399-302X.1996.tb00190.x