Application of B cell immortalization for the isolation of antibodies and B cell clones from vaccine and infection settings

The isolation and characterization of neutralizing antibodies from infection and vaccine settings informs future vaccine design, and methodologies that streamline the isolation of antibodies and the generation of B cell clones are of great interest. Retroviral transduction to express Bcl-6 and Bcl-x...

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Published in:Frontiers in immunology Vol. 13; p. 1087018
Main Authors: Boswell, Kristin L., Watkins, Timothy A., Cale, Evan M., Samsel, Jakob, Andrews, Sarah F., Ambrozak, David R., Driscoll, Jefferson I., Messina, Michael A., Narpala, Sandeep, Hopp, Christine S., Cagigi, Alberto, Casazza, Joseph P., Yamamoto, Takuya, Zhou, Tongqing, Schief, William R., Crompton, Peter D., Ledgerwood, Julie E., Connors, Mark, Gama, Lucio, Kwong, Peter D., McDermott, Adrian, Mascola, John R., Koup, Richard A.
Format: Journal Article
Language:English
Published: Switzerland Frontiers Media S.A 13.12.2022
Subjects:
Antibodies, Neutralizing
B cell
B-Lymphocytes
Cell Line
chronic infection
Clone Cells
HIV Seropositivity
HIV-1
Humans
immortalization
Immunology
malaria
vaccination
Vaccines
HIV-1
influenza
B cell
antibody
chronic infection
immortalization
malaria
vaccination
ISSN:1664-3224, 1664-3224
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Summary:The isolation and characterization of neutralizing antibodies from infection and vaccine settings informs future vaccine design, and methodologies that streamline the isolation of antibodies and the generation of B cell clones are of great interest. Retroviral transduction to express Bcl-6 and Bcl-xL and transform primary B cells has been shown to promote long-term B cell survival and antibody secretion in vitro , and can be used to isolate antibodies from memory B cells. However, application of this methodology to B cell subsets from different tissues and B cells from chronically infected individuals has not been well characterized. Here, we characterize Bcl-6/Bcl-xL B cell immortalization across multiple tissue types and B cell subsets in healthy and HIV-1 infected individuals, as well as individuals recovering from malaria. In healthy individuals, naïve and memory B cell subsets from PBMCs and tonsil tissue transformed with similar efficiencies, and displayed similar characteristics with respect to their longevity and immunoglobulin secretion. In HIV-1-viremic individuals or in individuals with recent malaria infections, the exhausted CD27 - CD21 - memory B cells transformed with lower efficiency, but the transformed B cells expanded and secreted IgG with similar efficiency. Importantly, we show that this methodology can be used to isolate broadly neutralizing antibodies from HIV-infected individuals. Overall, we demonstrate that Bcl-6/Bcl-xL B cell immortalization can be used to isolate antibodies and generate B cell clones from different B cell populations, albeit with varying efficiencies.
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Reviewed by: Christopher Sundling, Karolinska Institutet (KI), Sweden; Wataru Ise, Osaka University, Japan; Rajagopal Murugan, Leiden University Medical Center (LUMC), Netherlands
Edited by: Annalisa Ciabattini, University of Siena, Italy
This article was submitted to B Cell Biology, a section of the journal Frontiers in Immunology
ISSN:1664-3224
1664-3224
DOI:10.3389/fimmu.2022.1087018