Identification of direct transcriptional target genes of ExoS/ChvI two-component signaling in Sinorhizobium meliloti

The Sinorhizobium meliloti ExoS/ChvI two-component signaling pathway is required for the development of a nitrogen-fixing symbiosis between S. meliloti and its plant hosts. ExoS/ChvI also has important roles in regulating succinoglycan production, biofilm formation, motility, nutrient utilization, a...

Full description

Saved in:
Bibliographic Details
Published in:Journal of bacteriology Vol. 191; no. 22; pp. 6833 - 6842
Main Authors: Chen, Esther J, Fisher, Robert F, Perovich, Virginia M, Sabio, Erich A, Long, Sharon R
Format: Journal Article
Language:English
Published: United States 01.11.2009
Subjects:
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Bacterial Proteins - physiology
Base Sequence
DNA - metabolism
DNA Footprinting
Electrophoretic Mobility Shift Assay
Gene Expression Regulation, Bacterial - genetics
Gene Expression Regulation, Bacterial - physiology
Molecular Sequence Data
Oligonucleotide Array Sequence Analysis
Protein Binding
Sinorhizobium meliloti - genetics
Sinorhizobium meliloti - metabolism
Transcription Factors - genetics
Transcription Factors - metabolism
Transcription Factors - physiology
ISSN:1098-5530
Online Access:Get more information
Tags: Add Tag
No Tags, Be the first to tag this record!
Abstract The Sinorhizobium meliloti ExoS/ChvI two-component signaling pathway is required for the development of a nitrogen-fixing symbiosis between S. meliloti and its plant hosts. ExoS/ChvI also has important roles in regulating succinoglycan production, biofilm formation, motility, nutrient utilization, and the viability of free-living bacteria. Previous microarray experiments with an exoS96::Tn5 mutant indicated that ExoS/ChvI influences the expression of a few hundred genes, complicating the investigation of which downstream genes respond directly or indirectly to ExoS/ChvI regulation. To focus our study of ExoS/ChvI transcriptional target genes, we performed transcriptional profiling with chvI gain-of-function and reduced-function strains. The chvI gain-of-function strain that we used contains a dominant gain-of-function chvI allele in addition to wild-type chvI. We identified genes that, relative to their expression level in the wild type, are both upregulated in the chvI gain-of-function strain and downregulated in the reduced-function strain or vice versa. Guided by this focused set of genes, we performed gel mobility shift assays and demonstrated that ChvI directly binds the intergenic regions upstream of ropB1, SMb21440, and SMc01580. Furthermore, DNase I footprint analysis of the region upstream of SMc01580 identified a specific DNA sequence bound by ChvI and allowed the discovery of a possible motif for ChvI binding. Our results provide insight into the mechanism of how ExoS/ChvI regulates its downstream targets and lay a foundation for studying this conserved pathway with critical roles in free-living and symbiotic bacteria.
AbstractList The Sinorhizobium meliloti ExoS/ChvI two-component signaling pathway is required for the development of a nitrogen-fixing symbiosis between S. meliloti and its plant hosts. ExoS/ChvI also has important roles in regulating succinoglycan production, biofilm formation, motility, nutrient utilization, and the viability of free-living bacteria. Previous microarray experiments with an exoS96::Tn5 mutant indicated that ExoS/ChvI influences the expression of a few hundred genes, complicating the investigation of which downstream genes respond directly or indirectly to ExoS/ChvI regulation. To focus our study of ExoS/ChvI transcriptional target genes, we performed transcriptional profiling with chvI gain-of-function and reduced-function strains. The chvI gain-of-function strain that we used contains a dominant gain-of-function chvI allele in addition to wild-type chvI. We identified genes that, relative to their expression level in the wild type, are both upregulated in the chvI gain-of-function strain and downregulated in the reduced-function strain or vice versa. Guided by this focused set of genes, we performed gel mobility shift assays and demonstrated that ChvI directly binds the intergenic regions upstream of ropB1, SMb21440, and SMc01580. Furthermore, DNase I footprint analysis of the region upstream of SMc01580 identified a specific DNA sequence bound by ChvI and allowed the discovery of a possible motif for ChvI binding. Our results provide insight into the mechanism of how ExoS/ChvI regulates its downstream targets and lay a foundation for studying this conserved pathway with critical roles in free-living and symbiotic bacteria.
Author Perovich, Virginia M
Chen, Esther J
Fisher, Robert F
Sabio, Erich A
Long, Sharon R
Author_xml – sequence: 1
  givenname: Esther J
  surname: Chen
  fullname: Chen, Esther J
  email: echen@fullerton.edu
  organization: Department of Biological Science, Center for Applied Biotechnology Studies, College of Natural Sciences and Mathematics, California State University Fullerton, 800 N. State College Blvd., Fullerton, CA 92834-6850, USA. echen@fullerton.edu
– sequence: 2
  givenname: Robert F
  surname: Fisher
  fullname: Fisher, Robert F
– sequence: 3
  givenname: Virginia M
  surname: Perovich
  fullname: Perovich, Virginia M
– sequence: 4
  givenname: Erich A
  surname: Sabio
  fullname: Sabio, Erich A
– sequence: 5
  givenname: Sharon R
  surname: Long
  fullname: Long, Sharon R
BackLink https://www.ncbi.nlm.nih.gov/pubmed/19749054$$D View this record in MEDLINE/PubMed
BookMark eNo1kLtPwzAYxC0Eog-Y2JEntrSf4ziPEaoCRZUYCnPkOHZqlNjBdnj99aSiTCedfjrd3QydGmskQlcEFoTE-fLpbgGQ0SSC4gRNCRR5xBiFCZp5_wZAkoTF52hCiiwpgCVTFDa1NEErLXjQ1mCrcK2dFAEHx40XTvcHn7c4cNfIgBtppD9g6y-7W672HxscPm0kbNePVUzAXjcjrk2DtcE7bazb6x9b6aHDnWx1a4O-QGeKt15eHnWOXu_XL6vHaPv8sFndbiORpFmIVFFnIiecSkmqhOaJSlPFmKghBpoWfBzK8orGhEpV5awmtYiVAKCQcwJQxXN085fbO_s-SB_KTnsh25YbaQdfpmlKxyfoCF4fwaHqZF32TnfcfZf_P8W_rwxrLQ
CitedBy_id crossref_primary_10_1111_j_1365_294X_2012_05629_x
crossref_primary_10_1128_JB_00199_21
crossref_primary_10_1128_JB_01751_14
crossref_primary_10_1093_femsre_fuac048
crossref_primary_10_3390_ijms22126233
crossref_primary_10_1111_j_1574_6968_2012_02645_x
crossref_primary_10_1128_JB_06357_11
crossref_primary_10_3389_fpls_2021_678976
crossref_primary_10_1073_pnas_1400450111
crossref_primary_10_1128_JB_00501_17
crossref_primary_10_1094_MPMI_12_14_0407_R
crossref_primary_10_3389_fmicb_2023_1241143
crossref_primary_10_3389_fmicb_2022_1020932
crossref_primary_10_1111_mmi_14823
crossref_primary_10_3390_agronomy15051235
crossref_primary_10_1094_MPMI_09_13_0284_R
crossref_primary_10_1128_JB_05371_11
crossref_primary_10_1186_1471_2180_13_132
crossref_primary_10_1371_journal_pone_0135655
crossref_primary_10_1016_j_carbpol_2025_124010
crossref_primary_10_1093_femsle_fnx061
crossref_primary_10_3389_fpls_2014_00730
crossref_primary_10_1073_pnas_1917471117
crossref_primary_10_1128_JB_02626_14
crossref_primary_10_1128_mSystems_00035_17
crossref_primary_10_1091_mbc_E24_12_0546
crossref_primary_10_1371_journal_pgen_1010776
crossref_primary_10_1146_annurev_micro_120822_102714
crossref_primary_10_1073_pnas_1610724113
crossref_primary_10_1128_JB_01456_10
crossref_primary_10_1007_s11306_017_1210_2
crossref_primary_10_1128_JB_01676_09
crossref_primary_10_1128_JB_00665_17
crossref_primary_10_1007_s42994_024_00193_1
crossref_primary_10_1016_j_micpath_2023_106442
crossref_primary_10_1111_mmi_14119
crossref_primary_10_1128_jb_00182_25
ContentType Journal Article
DBID CGR
CUY
CVF
ECM
EIF
NPM
7X8
DOI 10.1128/JB.00734-09
DatabaseName Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
MEDLINE - Academic
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
MEDLINE - Academic
DatabaseTitleList MEDLINE - Academic
MEDLINE
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: 7X8
  name: MEDLINE - Academic
  url: https://search.proquest.com/medline
  sourceTypes: Aggregation Database
DeliveryMethod no_fulltext_linktorsrc
Discipline Biology
EISSN 1098-5530
EndPage 6842
ExternalDocumentID 19749054
Genre Research Support, U.S. Gov't, Non-P.H.S
Research Support, Non-U.S. Gov't
Journal Article
Research Support, N.I.H., Extramural
GrantInformation_xml – fundername: NIGMS NIH HHS
  grantid: R01-GM30692
GroupedDBID ---
-DZ
-~X
.55
.GJ
0R~
186
18M
1VV
29J
2WC
39C
3O-
4.4
53G
5GY
5RE
5VS
79B
85S
8WZ
9M8
A6W
AAGFI
ABPPZ
ACGFO
ACGOD
ACNCT
ACPRK
ADBBV
ADXHL
AENEX
AFFDN
AFFNX
AFRAH
AGCDD
AGVNZ
AI.
AIDAL
AJUXI
ALMA_UNASSIGNED_HOLDINGS
AOIJS
BAWUL
BKOMP
BTFSW
C1A
CGR
CJ0
CS3
CUY
CVF
DIK
DU5
E3Z
EBS
ECM
EIF
EJD
F5P
FRP
GX1
H13
HYE
HZ~
IH2
KQ8
L7B
MVM
NHB
NPM
O9-
OHT
OK1
P-O
P-S
P2P
PQQKQ
QZG
RHI
RNS
RPM
RSF
RXW
TAE
TR2
UHB
UKR
UPT
VH1
W8F
WH7
WHG
WOQ
X7M
Y6R
YQT
YR2
YZZ
ZCA
ZCG
ZGI
ZXP
ZY4
~02
~KM
7X8
ID FETCH-LOGICAL-c467t-f9d7c81a3ee1b4384f66f55cd020369a07358b3213efb85d1dc2fc00308a100b2
IEDL.DBID 7X8
ISICitedReferencesCount 43
ISICitedReferencesURI http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000271195300009&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
IngestDate Wed Oct 01 15:09:09 EDT 2025
Mon Jul 21 05:59:29 EDT 2025
IsDoiOpenAccess false
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 22
Language English
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c467t-f9d7c81a3ee1b4384f66f55cd020369a07358b3213efb85d1dc2fc00308a100b2
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
OpenAccessLink https://www.ncbi.nlm.nih.gov/pmc/articles/2772461
PMID 19749054
PQID 66639743
PQPubID 23479
PageCount 10
ParticipantIDs proquest_miscellaneous_66639743
pubmed_primary_19749054
PublicationCentury 2000
PublicationDate 2009-11-00
PublicationDateYYYYMMDD 2009-11-01
PublicationDate_xml – month: 11
  year: 2009
  text: 2009-11-00
PublicationDecade 2000
PublicationPlace United States
PublicationPlace_xml – name: United States
PublicationTitle Journal of bacteriology
PublicationTitleAlternate J Bacteriol
PublicationYear 2009
References 2793828 - J Bacteriol. 1989 Oct;171(10):5492-502
18284576 - Mol Microbiol. 2008 Mar;67(6):1292-306
8325480 - Genetics. 1993 Jun;134(2):435-44
8144468 - J Bacteriol. 1994 Apr;176(7):1997-2002
16629750 - FEMS Microbiol Ecol. 2006 May;56(2):195-206
10542182 - J Bacteriol. 1999 Nov;181(21):6788-96
8710923 - Proc Natl Acad Sci U S A. 1996 Aug 6;93(16):8636-41
7635814 - J Bacteriol. 1995 Aug;177(15):4289-96
8331671 - J Mol Biol. 1993 Jul 5;232(1):67-78
6330025 - J Bacteriol. 1984 Jul;159(1):125-9
11474104 - Science. 2001 Jul 27;293(5530):668-72
16735738 - Microbiology. 2006 Jun;152(Pt 6):1751-63
15342573 - J Bacteriol. 2004 Sep;186(18):6042-9
9802033 - Microbiology. 1998 Oct;144 ( Pt 10):2915-24
10948423 - Biotechniques. 2000 Aug;29(2):240-2, 244-5
10361286 - Mol Microbiol. 1999 May;32(4):837-49
8589411 - Mol Plant Microbe Interact. 1995 Jul-Aug;8(4):549-59
16816173 - J Bacteriol. 2006 Jul;188(14):5003-13
18631237 - Mol Microbiol. 2008 Sep;69(5):1290-303
3013840 - J Bacteriol. 1986 Jul;167(1):66-72
15542588 - Proc Natl Acad Sci U S A. 2004 Nov 23;101(47):16636-41
14769477 - FEMS Microbiol Lett. 2004 Feb 9;231(1):131-6
12813079 - J Bacteriol. 2003 Jul;185(13):3853-62
18375818 - Microbiology. 2008 Apr;154(Pt 4):1258-70
7559334 - J Bacteriol. 1995 Oct;177(19):5485-94
15263816 - J Mol Microbiol Biotechnol. 2004;7(3):115-32
12653992 - Eur J Biochem. 2003 Apr;270(7):1365-80
16980496 - J Bacteriol. 2006 Oct;188(19):6932-42
15205418 - J Bacteriol. 2004 Jul;186(13):4168-76
9701808 - Mol Microbiol. 1998 Jul;29(1):125-38
11497462 - Mol Plant Microbe Interact. 2001 Aug;14(8):1016-25
17462014 - Mol Microbiol. 2007 May;64(3):647-64
3862129 - Proc Natl Acad Sci U S A. 1985 Sep;82(18):6231-5
15292148 - J Bacteriol. 2004 Aug;186(16):5460-72
9748453 - J Bacteriol. 1998 Oct;180(19):5183-91
9422587 - J Bacteriol. 1998 Jan;180(1):20-6
6274841 - J Bacteriol. 1982 Jan;149(1):114-22
7873780 - Mol Plant Microbe Interact. 1994 Nov-Dec;7(6):758-65
17632573 - Nat Rev Microbiol. 2007 Aug;5(8):619-33
18842098 - Mol Plant Microbe Interact. 2008 Nov;21(11):1498-509
17158704 - Microbiol Mol Biol Rev. 2006 Dec;70(4):910-38
11918800 - Mol Microbiol. 2002 Mar;43(5):1115-27
8407839 - J Bacteriol. 1993 Oct;175(20):6614-25
16535451 - Appl Environ Microbiol. 1996 Nov;62(11):4260-2
15187185 - Microbiol Mol Biol Rev. 2004 Jun;68(2):280-300
18310071 - J Biol Chem. 2008 Apr 18;283(16):10415-24
10485295 - Mol Gen Genet. 1999 Jul;261(6):1032-44
11274120 - J Bacteriol. 2001 Apr;183(8):2595-604
9204561 - Mol Plant Microbe Interact. 1997 Jul;10(5):550-9
11902715 - Mol Microbiol. 1997 Jul;25(1):117-34
2842307 - J Bacteriol. 1988 Sep;170(9):4249-56
12218184 - Proc Natl Acad Sci U S A. 2002 Sep 17;99(19):12369-74
10894742 - J Bacteriol. 2000 Aug;182(15):4310-8
3288541 - Genes Dev. 1988 Mar;2(3):282-93
12218183 - Proc Natl Acad Sci U S A. 2002 Sep 17;99(19):12375-80
19395488 - J Bacteriol. 2009 Jul;191(13):4372-82
References_xml – reference: 12218184 - Proc Natl Acad Sci U S A. 2002 Sep 17;99(19):12369-74
– reference: 11497462 - Mol Plant Microbe Interact. 2001 Aug;14(8):1016-25
– reference: 3288541 - Genes Dev. 1988 Mar;2(3):282-93
– reference: 10894742 - J Bacteriol. 2000 Aug;182(15):4310-8
– reference: 18842098 - Mol Plant Microbe Interact. 2008 Nov;21(11):1498-509
– reference: 17462014 - Mol Microbiol. 2007 May;64(3):647-64
– reference: 10542182 - J Bacteriol. 1999 Nov;181(21):6788-96
– reference: 8589411 - Mol Plant Microbe Interact. 1995 Jul-Aug;8(4):549-59
– reference: 10361286 - Mol Microbiol. 1999 May;32(4):837-49
– reference: 18310071 - J Biol Chem. 2008 Apr 18;283(16):10415-24
– reference: 15342573 - J Bacteriol. 2004 Sep;186(18):6042-9
– reference: 12218183 - Proc Natl Acad Sci U S A. 2002 Sep 17;99(19):12375-80
– reference: 10948423 - Biotechniques. 2000 Aug;29(2):240-2, 244-5
– reference: 15542588 - Proc Natl Acad Sci U S A. 2004 Nov 23;101(47):16636-41
– reference: 17158704 - Microbiol Mol Biol Rev. 2006 Dec;70(4):910-38
– reference: 3013840 - J Bacteriol. 1986 Jul;167(1):66-72
– reference: 7873780 - Mol Plant Microbe Interact. 1994 Nov-Dec;7(6):758-65
– reference: 15292148 - J Bacteriol. 2004 Aug;186(16):5460-72
– reference: 12653992 - Eur J Biochem. 2003 Apr;270(7):1365-80
– reference: 16816173 - J Bacteriol. 2006 Jul;188(14):5003-13
– reference: 15205418 - J Bacteriol. 2004 Jul;186(13):4168-76
– reference: 9204561 - Mol Plant Microbe Interact. 1997 Jul;10(5):550-9
– reference: 8710923 - Proc Natl Acad Sci U S A. 1996 Aug 6;93(16):8636-41
– reference: 12813079 - J Bacteriol. 2003 Jul;185(13):3853-62
– reference: 9422587 - J Bacteriol. 1998 Jan;180(1):20-6
– reference: 8144468 - J Bacteriol. 1994 Apr;176(7):1997-2002
– reference: 14769477 - FEMS Microbiol Lett. 2004 Feb 9;231(1):131-6
– reference: 9701808 - Mol Microbiol. 1998 Jul;29(1):125-38
– reference: 19395488 - J Bacteriol. 2009 Jul;191(13):4372-82
– reference: 6330025 - J Bacteriol. 1984 Jul;159(1):125-9
– reference: 8407839 - J Bacteriol. 1993 Oct;175(20):6614-25
– reference: 9802033 - Microbiology. 1998 Oct;144 ( Pt 10):2915-24
– reference: 7559334 - J Bacteriol. 1995 Oct;177(19):5485-94
– reference: 8325480 - Genetics. 1993 Jun;134(2):435-44
– reference: 15263816 - J Mol Microbiol Biotechnol. 2004;7(3):115-32
– reference: 11474104 - Science. 2001 Jul 27;293(5530):668-72
– reference: 16735738 - Microbiology. 2006 Jun;152(Pt 6):1751-63
– reference: 18631237 - Mol Microbiol. 2008 Sep;69(5):1290-303
– reference: 18284576 - Mol Microbiol. 2008 Mar;67(6):1292-306
– reference: 11902715 - Mol Microbiol. 1997 Jul;25(1):117-34
– reference: 18375818 - Microbiology. 2008 Apr;154(Pt 4):1258-70
– reference: 8331671 - J Mol Biol. 1993 Jul 5;232(1):67-78
– reference: 2842307 - J Bacteriol. 1988 Sep;170(9):4249-56
– reference: 3862129 - Proc Natl Acad Sci U S A. 1985 Sep;82(18):6231-5
– reference: 16629750 - FEMS Microbiol Ecol. 2006 May;56(2):195-206
– reference: 9748453 - J Bacteriol. 1998 Oct;180(19):5183-91
– reference: 11918800 - Mol Microbiol. 2002 Mar;43(5):1115-27
– reference: 17632573 - Nat Rev Microbiol. 2007 Aug;5(8):619-33
– reference: 16535451 - Appl Environ Microbiol. 1996 Nov;62(11):4260-2
– reference: 10485295 - Mol Gen Genet. 1999 Jul;261(6):1032-44
– reference: 6274841 - J Bacteriol. 1982 Jan;149(1):114-22
– reference: 16980496 - J Bacteriol. 2006 Oct;188(19):6932-42
– reference: 15187185 - Microbiol Mol Biol Rev. 2004 Jun;68(2):280-300
– reference: 11274120 - J Bacteriol. 2001 Apr;183(8):2595-604
– reference: 2793828 - J Bacteriol. 1989 Oct;171(10):5492-502
– reference: 7635814 - J Bacteriol. 1995 Aug;177(15):4289-96
SSID ssj0014452
Score 2.162208
Snippet The Sinorhizobium meliloti ExoS/ChvI two-component signaling pathway is required for the development of a nitrogen-fixing symbiosis between S. meliloti and its...
SourceID proquest
pubmed
SourceType Aggregation Database
Index Database
StartPage 6833
SubjectTerms Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Bacterial Proteins - physiology
Base Sequence
DNA - metabolism
DNA Footprinting
Electrophoretic Mobility Shift Assay
Gene Expression Regulation, Bacterial - genetics
Gene Expression Regulation, Bacterial - physiology
Molecular Sequence Data
Oligonucleotide Array Sequence Analysis
Protein Binding
Sinorhizobium meliloti - genetics
Sinorhizobium meliloti - metabolism
Transcription Factors - genetics
Transcription Factors - metabolism
Transcription Factors - physiology
Title Identification of direct transcriptional target genes of ExoS/ChvI two-component signaling in Sinorhizobium meliloti
URI https://www.ncbi.nlm.nih.gov/pubmed/19749054
https://www.proquest.com/docview/66639743
Volume 191
WOSCitedRecordID wos000271195300009&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
hasFullText
inHoldings 1
isFullTextHit
isPrint
link http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1LS8NAEF6qVfDi-1Gfe_C6NMlumgQE0WJRwVKoQm9hsw8NtNnaplX_vbN54E0PXnIIu8syO_lmsvPNDEKX8FeVKEcwIhmXhHHHIYmQPmFacdCPgErFimYTQb8fjkbRoIGu6lwYS6usMbEAammEvSNvg5sNppPR6-k7sT2jbGy1aqCxgpoUHBlL6ApGPzEExnyvysgDEG4_3toy2ZQR5xdvsrAqva3_7WcbbVbeJL4pj38HNVS2i9bL_pJfe6hKw9XVvRw2GpcmDOfWRNWAASuUhHD8apHPDrv7NMN29235gPMPQyzv3GSwFLZ0D24z2HGa4WGamZnl7CXpYoInapyOTZ7uo5fe3XP3nlRtFogAlMyJjmQgQpdTpdyE0ZDpTkf7vpBFkDLiIDQ_TKjnUqWT0JeuFJ4WRaEb7jpO4h2g1Qw2cYRwqBWMB5xQYPXA9nOmuBd1POXawn1KtNBFLcwY1NjGJnimzGIe1-JsocPyPOJpWW0jduF1BI7l8Z9zT9BGEespMgVPUVPDB6zO0JpY5ul8dl5oBzz7g6dv_s7I-g
linkProvider ProQuest
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Identification+of+direct+transcriptional+target+genes+of+ExoS%2FChvI+two-component+signaling+in+Sinorhizobium+meliloti&rft.jtitle=Journal+of+bacteriology&rft.au=Chen%2C+Esther+J&rft.au=Fisher%2C+Robert+F&rft.au=Perovich%2C+Virginia+M&rft.au=Sabio%2C+Erich+A&rft.date=2009-11-01&rft.eissn=1098-5530&rft.volume=191&rft.issue=22&rft.spage=6833&rft.epage=6842&rft_id=info:doi/10.1128%2FJB.00734-09&rft.externalDBID=NO_FULL_TEXT