The characterization of normal thyroid tissue by micro-FTIR spectroscopy

In this paper, we report the spectral patterns of normal human thyroid tissue and methodology to interpret hyperspectral imaging data and protein conformational changes observed therein. Raw image datasets were imported into software written in-house in the MATLAB environment and processed to yield...

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Bibliographic Details
Published in:	Analyst (London) Vol. 138; no. 23; p. 7094
Main Authors:	Pereira, Thiago M, Zezell, Denise M, Bird, Benjamin, Miljković, Milos, Diem, Max
Format:	Journal Article
Language:	English
Published:	England 07.12.2013
Subjects:	Humans Spectroscopy, Fourier Transform Infrared - methods Thyroid Gland - chemistry Thyroid Hormones - analysis Tissue Array Analysis
ISSN:	1364-5528, 1364-5528
Online Access:	Get more information
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Summary:	In this paper, we report the spectral patterns of normal human thyroid tissue and methodology to interpret hyperspectral imaging data and protein conformational changes observed therein. Raw image datasets were imported into software written in-house in the MATLAB environment and processed to yield pseudo-color images of the tissue sections. All spectra were vector normalized, noise-filtered, and corrected for water-vapour contributions and scattering effects before being subjected to Hierarchical Cluster Analysis (HCA) and correlated with histological structures obtained from images of H&E-stained parallel tissue sections. We successfully identified a protein structural heterogeneity that can be correlated with the spatially resolved amount of iodine in the thyroglobulin structure of colloids and follicular cells.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1364-5528 1364-5528
DOI:	10.1039/c3an00296a