Signal averaged laser Doppler measurements of activation–flow coupling in the rat forepaw somatosensory cortex

Regional alterations in cerebral blood flow (CBF) are widely used as a surrogate for neuronal function based on an intact coupling between changes in regional CBF and metabolism, activation–flow coupling (AFC). To further investigate parameters affecting AFC, we have implemented a rat model with ele...

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Vydáno v:Brain research Ročník 796; číslo 1; s. 91 - 98
Hlavní autoři: Detre, John A, Ances, Beau M, Takahashi, Kazushi, Greenberg, Joel H
Médium: Journal Article
Jazyk:angličtina
Vydáno: London Elsevier B.V 15.06.1998
Amsterdam Elsevier
New York, NY
Témata:
Animals
Biological and medical sciences
Blood Flow Velocity - physiology
Brain Mapping - methods
Cerebrovascular Circulation - physiology
Electric Stimulation
Flow metabolic coupling
Forelimb - physiology
Fundamental and applied biological sciences. Psychology
Laser Doppler flowmetry
Male
Rats
Rats, Sprague-Dawley
Signal Processing, Computer-Assisted
Somatosensory cortex
Somatosensory Cortex - diagnostic imaging
Somatosensory Cortex - physiology
Somesthesis and somesthetic pathways (proprioception, exteroception, nociception); interoception; electrolocation. Sensory receptors
Ultrasonography
Vertebrates: nervous system and sense organs
Laser Doppler flowmetry
Somatosensory cortex
Flow metabolic coupling
Regional blood flow
Vertebrata
Laser dopplerometry
Mammalia
Rat
Somesthetic pathway
Rodentia
Central nervous system
Hemodynamics
Brain (vertebrata)
ISSN:0006-8993, 1872-6240
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Shrnutí:Regional alterations in cerebral blood flow (CBF) are widely used as a surrogate for neuronal function based on an intact coupling between changes in regional CBF and metabolism, activation–flow coupling (AFC). To further investigate parameters affecting AFC, we have implemented a rat model with electrical forepaw stimulation under α-chloralose anesthesia using laser Doppler (LD) measurements of flow parameters through thinned skull over contralateral somatosensory cortex. Signal averaging of the LD response was used to improve reproducibility. A characteristic flow response to electrical forepaw stimulation was reliably recorded from the somatosensory cortex using signal averaging. Stimulation at 5 Hz maximized the LD response, and constant current stimulation up to 1 mA did not induce changes in systemic blood pressure. The shape of the flow response consisted of an initial peak followed by a steady state plateau phase which was observed for stimulation durations longer than 4 s. When individual LD parameters of velocity, red blood cell concentration (CRBC), and cerebral blood flow (CBF) were compared, changes in LD CBF were primarily attributable to changes in LD velocity rather than LD CRBC. This finding was also observed during hypercapnia. Characterization of AFC in the model provides a background for future studies of the effects of pharmacological manipulation or pathophysiological states.
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ISSN:0006-8993
1872-6240
DOI:10.1016/S0006-8993(98)00322-9