Intraflagellar transport genes are essential for differentiation and survival of vertebrate sensory neurons

Cilia play diverse roles in vertebrate and invertebrate sensory neurons. We show that a mutation of the zebrafish oval (ovl) locus affects a component of the ciliary transport (IFT) mechanism, the IFT88 polypeptide. In mutant retina, cilia are generated but not maintained, producing the absence of p...

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Bibliographic Details
Published in:Neuron (Cambridge, Mass.) Vol. 42; no. 5; p. 703
Main Authors: Tsujikawa, Motokazu, Malicki, Jarema
Format: Journal Article
Language:English
Published: United States 10.06.2004
Subjects:
Algal Proteins - physiology
Animals
Animals, Genetically Modified
Biological Transport - genetics
Biological Transport - physiology
Blotting, Western - methods
Body Patterning - genetics
Body Patterning - physiology
Carrier Proteins - genetics
Cell Differentiation - genetics
Cell Differentiation - physiology
Cell Survival - genetics
Cell Survival - physiology
Chromosome Mapping
Cilia - metabolism
Cloning, Molecular - methods
Embryo, Mammalian
Embryo, Nonmammalian
Flagella - metabolism
Gene Expression Regulation, Developmental
Green Fluorescent Proteins
Humans
Immunohistochemistry - methods
In Situ Hybridization - methods
In Situ Nick-End Labeling - methods
Luminescent Proteins - metabolism
Mice
Microinjections
Microscopy, Electron
Molecular Sequence Data
Mutation
Neurons, Afferent - cytology
Neurons, Afferent - ultrastructure
Phalloidine
Phenotype
Photic Stimulation
Plant Proteins
Propidium
Protozoan Proteins - genetics
Protozoan Proteins - physiology
Retina - metabolism
Retina - ultrastructure
Retinal Cone Photoreceptor Cells - metabolism
Retinal Rod Photoreceptor Cells - metabolism
Reverse Transcriptase Polymerase Chain Reaction - methods
RNA, Messenger - biosynthesis
Rod Opsins - metabolism
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Tubulin - metabolism
Zebrafish
ISSN:0896-6273
Online Access:Get more information
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Description
Summary:Cilia play diverse roles in vertebrate and invertebrate sensory neurons. We show that a mutation of the zebrafish oval (ovl) locus affects a component of the ciliary transport (IFT) mechanism, the IFT88 polypeptide. In mutant retina, cilia are generated but not maintained, producing the absence of photoreceptor outer segments. A loss of cilia also occurs in auditory hair cells and olfactory sensory neurons. In all three sense organs, cilia defects are followed by degeneration of sensory cells. Similar phenotypes are induced by the absence of the IFT complex B polypeptides, ift52 and ift57, but not by the loss of complex A protein, ift140. The degeneration of mutant photoreceptor cells is caused, at least partially, by the ectopic accumulation of opsins. These studies reveal an essential role for IFT genes in vertebrate sensory neurons and implicate the molecular components of intraflagellar transport in degenerative disorders of these cells.
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ISSN:0896-6273
DOI:10.1016/S0896-6273(04)00268-5