Application of the Checkerboard Immunoblotting Technique to the Quantification of Host Biomarkers in Gingival Crevicular Fluid

Background: The aim of this study was to describe the development and validation of the checkerboard immunoblotting (CBIB) technique for the high‐throughput quantification of multiple inflammatory mediators in gingival crevicular fluid (GCF) samples. Methods: Monoclonal antibodies were used to bind...

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Bibliographic Details
Published in:	Journal of periodontology (1970) Vol. 80; no. 3; pp. 447 - 456
Main Authors:	Teles, Ricardo P., Sakellari, Dimitra, Konstantinidis, Antonis, Socransky, Sigmund S., Haffajee, Anne D.
Format:	Journal Article
Language:	English
Published:	Chicago, IL American Academy of Periodontology 01.03.2009
Subjects:	Adult Antibodies, Immobilized Biological and medical sciences Biomarkers - analysis Chemokines Chronic Periodontitis - metabolism Cross Reactions cytokines Dental Plaque - metabolism Enzyme-Linked Immunosorbent Assay - methods Facial bones, jaws, teeth, parodontium: diseases, semeiology Female gingival crevicular fluid Gingival Crevicular Fluid - chemistry Gingival Hemorrhage - metabolism Gingival Recession - metabolism Gingivitis - metabolism Humans Immunoblotting - methods Interleukin-1beta - analysis Interleukin-8 - analysis Luminescence Male Matrix Metalloproteinase 8 - analysis matrix metalloproteinases Medical sciences Membranes, Artificial Middle Aged Non tumoral diseases Otorhinolaryngology. Stomatology periodontal diseases Periodontal Pocket - metabolism Periodontium - metabolism Polyvinyls Sensitivity and Specificity Software Stomatology Crevicular fluid Cytokine Biological marker Dentistry Chemokine Periodontal disease gingival crevicular fluid cytokines Technique periodontal diseases Application matrix metalloproteinases Chemokines Gingival fluid
ISSN:	0022-3492, 1943-3670
Online Access:	Get full text
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