Organophosphorus Hydrolase-Based Biosensor for Direct Detection of Paraoxon Using Silica-Coated Magnetic Nanoparticles

Rapid detection of organophosphorous (OP) compounds such as paraoxon would allow taking immediate decision on efficient decontamination procedures and could prevent further damage and potential casualties. In the present study, a biosensor based on nanomagnet-silica core-shell conjugated to organoph...

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Published in:Applied biochemistry and biotechnology Vol. 176; no. 2; pp. 359 - 371
Main Authors: Khaksarinejad, Reza, Mohsenifar, Afshin, Rahmani-Cherati, Tavoos, Karami, Rezvan, Tabatabaei, Meisam
Format: Journal Article
Language:English
Published: New York Springer-Verlag 01.05.2015
Springer US
Springer Nature B.V
Subjects:
active sites
Aryldialkylphosphatase - chemistry
Bacterial Proteins - chemistry
Biochemistry
Biosensing Techniques - methods
Biosensors
Biotechnology
Chemistry
Chemistry and Materials Science
Coefficient of variation
Coumarins - chemistry
Decontamination
detection limit
Kinetics
Magnetite Nanoparticles - chemistry
Nanoparticles
paraoxon
Paraoxon - analysis
Pseudomonas - enzymology
Silica
Silicon Dioxide - chemistry
Organophosphorous hydrolase
Kinetics
Nanobiosensor
Nanomagnet-silica core-shell
Paraoxon
ISSN:0273-2289, 1559-0291, 1559-0291
Online Access:Get full text
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Summary:Rapid detection of organophosphorous (OP) compounds such as paraoxon would allow taking immediate decision on efficient decontamination procedures and could prevent further damage and potential casualties. In the present study, a biosensor based on nanomagnet-silica core-shell conjugated to organophosphorous hydrolase (OPH) enzyme was designed for detection of paraoxon. Coumarin1, a competitive inhibitor of the OPH enzyme, was used as a fluorescence-generating molecule. Upon excitation of cumarin1 located at the active site of the enzyme, i.e., OPH, the emitted radiations were intensified due to the mirroring effect of the nanomagnet-silica core-shell conjugated to the enzyme. In presence of paraoxon and consequent competition with the fluorophore in occupying enzyme’s active site, a significant reduction in emitted radiations was observed. This reduction was proportional to paraoxon concentration in the sample. The method worked in the 10- to 250-nM concentration range had a low standard deviation (with a coefficient of variation (CV) of 6–10 %), and the detection limit was as low as 5 × 10⁻⁶ μM.
Bibliography:http://dx.doi.org/10.1007/s12010-015-1579-1
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ISSN:0273-2289
1559-0291
1559-0291
DOI:10.1007/s12010-015-1579-1