Direct Neuronal Reprogramming: Achievements, Hurdles, and New Roads to Success

The ability to directly reprogram mature cells to alternative fates challenges concepts of how cell identities are maintained, erased, and acquired. Recent advances in understanding and overcoming hurdles to direct neuronal conversion have provided new insights into mechanisms that maintain cell ide...

Full description

Saved in:
Bibliographic Details
Published in:Cell stem cell Vol. 21; no. 1; p. 18
Main Authors: Gascón, Sergio, Masserdotti, Giacomo, Russo, Gianluca Luigi, Götz, Magdalena
Format: Journal Article
Language:English
Published: United States 06.07.2017
Subjects:
Animals
Cellular Reprogramming
Cellular Reprogramming Techniques - methods
Humans
Neurons - cytology
Neurons - metabolism
stem cells
brain repair
direct reprogramming
iPSC
neurogenesis
neuron
transdifferentiation
metabolism
conversion
ISSN:1875-9777, 1875-9777
Online Access:Get more information
Tags: Add Tag
No Tags, Be the first to tag this record!
Abstract The ability to directly reprogram mature cells to alternative fates challenges concepts of how cell identities are maintained, erased, and acquired. Recent advances in understanding and overcoming hurdles to direct neuronal conversion have provided new insights into mechanisms that maintain cell identity programs and have enabled high efficiency reprogramming in vivo. We discuss key cell-intrinsic molecular and metabolic constraints that influence the establishment of a new identity as well as environmental inputs from injured brains that favor or harm the conversion process. Finally, we outline the challenges ahead with a particular focus on direct neuronal reprogramming in vivo.
AbstractList The ability to directly reprogram mature cells to alternative fates challenges concepts of how cell identities are maintained, erased, and acquired. Recent advances in understanding and overcoming hurdles to direct neuronal conversion have provided new insights into mechanisms that maintain cell identity programs and have enabled high efficiency reprogramming in vivo. We discuss key cell-intrinsic molecular and metabolic constraints that influence the establishment of a new identity as well as environmental inputs from injured brains that favor or harm the conversion process. Finally, we outline the challenges ahead with a particular focus on direct neuronal reprogramming in vivo.The ability to directly reprogram mature cells to alternative fates challenges concepts of how cell identities are maintained, erased, and acquired. Recent advances in understanding and overcoming hurdles to direct neuronal conversion have provided new insights into mechanisms that maintain cell identity programs and have enabled high efficiency reprogramming in vivo. We discuss key cell-intrinsic molecular and metabolic constraints that influence the establishment of a new identity as well as environmental inputs from injured brains that favor or harm the conversion process. Finally, we outline the challenges ahead with a particular focus on direct neuronal reprogramming in vivo.
The ability to directly reprogram mature cells to alternative fates challenges concepts of how cell identities are maintained, erased, and acquired. Recent advances in understanding and overcoming hurdles to direct neuronal conversion have provided new insights into mechanisms that maintain cell identity programs and have enabled high efficiency reprogramming in vivo. We discuss key cell-intrinsic molecular and metabolic constraints that influence the establishment of a new identity as well as environmental inputs from injured brains that favor or harm the conversion process. Finally, we outline the challenges ahead with a particular focus on direct neuronal reprogramming in vivo.
Author Gascón, Sergio
Masserdotti, Giacomo
Götz, Magdalena
Russo, Gianluca Luigi
Author_xml – sequence: 1
  givenname: Sergio
  surname: Gascón
  fullname: Gascón, Sergio
  organization: Institute of Stem Cell Research, Helmholtz Center Munich and Physiological Genomics, Biomedical Center, Ludwig-Maximilians-University Munich, Großhadernerstrasse 9, 82152 Planegg/Munich, Germany
– sequence: 2
  givenname: Giacomo
  surname: Masserdotti
  fullname: Masserdotti, Giacomo
  organization: Institute of Stem Cell Research, Helmholtz Center Munich and Physiological Genomics, Biomedical Center, Ludwig-Maximilians-University Munich, Großhadernerstrasse 9, 82152 Planegg/Munich, Germany
– sequence: 3
  givenname: Gianluca Luigi
  surname: Russo
  fullname: Russo, Gianluca Luigi
  organization: Institute of Stem Cell Research, Helmholtz Center Munich and Physiological Genomics, Biomedical Center, Ludwig-Maximilians-University Munich, Großhadernerstrasse 9, 82152 Planegg/Munich, Germany; Graduate School of Systemic Neurosciences, Biomedical Center, Ludwig-Maximilians-University Munich, 82152 Planegg, Germany
– sequence: 4
  givenname: Magdalena
  surname: Götz
  fullname: Götz, Magdalena
  email: magdalena.goetz@helmholtz-muenchen.de
  organization: Institute of Stem Cell Research, Helmholtz Center Munich and Physiological Genomics, Biomedical Center, Ludwig-Maximilians-University Munich, Großhadernerstrasse 9, 82152 Planegg/Munich, Germany; SYNERGY, Excellence Cluster of Systems Neurology, Biomedical Center, Ludwig-Maximilians-University Munich, 81377 Munich, Germany. Electronic address: magdalena.goetz@helmholtz-muenchen.de
BackLink https://www.ncbi.nlm.nih.gov/pubmed/28686866$$D View this record in MEDLINE/PubMed
BookMark eNpNUEtPwzAYi9AQe8Af4IBy5EBLkuZVbtN4DGka0oBzlaZfR6emHUkL4t9TxJCQD_bBtixP0ahpG0DonJKYEiqvd3HowMWMUBUTGRNKj9CEaiWiVCk1-qfHaBrCjhChKFEnaMy0_IGcoPVt5cF2eA29bxtT4w3sfbv1xrmq2d7guX2r4AMcNF24wsveFzUMwjTFEPnEm9YUAXctfu6thRBO0XFp6gBnB56h1_u7l8UyWj09PC7mq8hyKbtIa2qF0CJhkiupWQKlLAnLyxKklYYDSaUwwFnKaEpKBgYoqELwPJWJTgybocvf3mHsew-hy1wVLNS1aaDtQ0ZTqhLJheKD9eJg7XMHRbb3lTP-K_v7gH0Dbm1f_g
CitedBy_id crossref_primary_10_1016_j_gde_2018_05_011
crossref_primary_10_1089_cell_2021_0172
crossref_primary_10_3389_fgene_2020_563798
crossref_primary_10_1016_j_semcancer_2019_09_008
crossref_primary_10_1111_cns_14000
crossref_primary_10_4103_1673_5374_391313
crossref_primary_10_3389_fneur_2019_00630
crossref_primary_10_3390_biomedicines10102598
crossref_primary_10_3389_fcell_2021_681087
crossref_primary_10_3390_ijms23010274
crossref_primary_10_3390_diseases8040042
crossref_primary_10_3389_fnins_2022_878875
crossref_primary_10_3390_cells13141223
crossref_primary_10_3390_ijms25115595
crossref_primary_10_3390_ijms19030770
crossref_primary_10_1038_s41419_018_0891_4
crossref_primary_10_1038_s41556_020_0490_3
crossref_primary_10_1016_j_stem_2021_09_002
crossref_primary_10_3389_fncel_2023_1237641
crossref_primary_10_3233_JAD_180422
crossref_primary_10_1002_advs_202308032
crossref_primary_10_1089_scd_2018_0233
crossref_primary_10_3390_biomedicines11112913
crossref_primary_10_1146_annurev_cellbio_100818_125127
crossref_primary_10_1038_s41419_019_1887_4
crossref_primary_10_3389_fnagi_2021_689276
crossref_primary_10_7759_cureus_74300
crossref_primary_10_1038_s41433_024_03441_2
crossref_primary_10_1016_j_chembiol_2019_05_007
crossref_primary_10_1016_j_neuron_2019_08_009
crossref_primary_10_1038_s41593_018_0168_3
crossref_primary_10_1093_nar_gkz273
crossref_primary_10_1186_s10020_023_00691_w
crossref_primary_10_1146_annurev_genet_071719_023616
crossref_primary_10_1038_s41586_020_2388_4
crossref_primary_10_3389_fnmol_2021_642016
crossref_primary_10_1016_j_neuron_2023_12_020
crossref_primary_10_1038_d41586_020_01817_4
crossref_primary_10_1103_PhysRevResearch_6_023309
crossref_primary_10_1016_j_molmed_2020_08_006
crossref_primary_10_1002_dneu_22880
crossref_primary_10_3389_fbioe_2021_658498
crossref_primary_10_3389_fvets_2020_575938
crossref_primary_10_1038_s41540_022_00218_9
crossref_primary_10_1186_s13036_019_0144_9
crossref_primary_10_4103_NRR_NRR_D_23_01612
crossref_primary_10_3389_fbioe_2022_799152
crossref_primary_10_1016_j_arr_2022_101655
crossref_primary_10_2302_kjm_2019_0008_OA
crossref_primary_10_1002_dvdy_24655
crossref_primary_10_1134_S1062360422040026
crossref_primary_10_3390_cells13090745
crossref_primary_10_1038_s41580_021_00335_z
crossref_primary_10_1016_j_jconrel_2020_03_025
crossref_primary_10_1039_D1BM00400J
crossref_primary_10_1186_s42494_022_00104_1
crossref_primary_10_1186_s13059_025_03556_z
crossref_primary_10_1038_s41551_022_00963_6
crossref_primary_10_1016_j_semcdb_2019_04_001
crossref_primary_10_1016_j_semcdb_2018_12_003
crossref_primary_10_1016_j_pneurobio_2018_06_004
crossref_primary_10_3389_fcell_2021_657337
crossref_primary_10_3389_fcell_2021_728352
crossref_primary_10_3389_fcell_2022_927555
crossref_primary_10_1242_dev_193631
crossref_primary_10_1016_j_lfs_2018_07_030
crossref_primary_10_3389_fneur_2020_00344
crossref_primary_10_1096_fj_201801110RR
crossref_primary_10_1038_s41586_018_0087_1
crossref_primary_10_1186_s40824_022_00280_8
crossref_primary_10_1073_pnas_2107339119
crossref_primary_10_3390_biomedicines10020399
crossref_primary_10_1016_j_ceb_2017_12_004
crossref_primary_10_1002_adma_201705684
crossref_primary_10_1016_j_stem_2019_05_016
crossref_primary_10_3390_cells12040528
crossref_primary_10_1038_s41598_017_18700_4
crossref_primary_10_1038_s42255_018_0028_1
crossref_primary_10_3390_cells8060571
crossref_primary_10_1002_adma_202206933
crossref_primary_10_4103_1673_5374_265185
crossref_primary_10_1016_j_pneurobio_2018_04_003
crossref_primary_10_3389_fcell_2018_00016
crossref_primary_10_1016_j_neuropharm_2018_09_032
crossref_primary_10_1038_d41586_020_00479_6
crossref_primary_10_1007_s00441_017_2738_1
crossref_primary_10_3390_ijms222413577
crossref_primary_10_1002_dneu_22951
crossref_primary_10_3390_cells12091253
crossref_primary_10_1002_adfm_201910250
crossref_primary_10_1016_j_cub_2021_08_049
crossref_primary_10_3390_biomedicines12030663
crossref_primary_10_3390_cells11213435
crossref_primary_10_1038_s42003_018_0070_2
crossref_primary_10_3233_JAD_230119
crossref_primary_10_3389_fnins_2022_853911
crossref_primary_10_3389_fnmol_2023_1110356
crossref_primary_10_1007_s12975_022_01078_5
crossref_primary_10_3389_fnins_2020_546484
crossref_primary_10_1007_s12264_021_00751_3
crossref_primary_10_1038_s41467_019_14235_6
crossref_primary_10_1073_pnas_2307972120
crossref_primary_10_1111_ejn_15742
crossref_primary_10_1038_s41598_019_53007_6
crossref_primary_10_4103_NRR_NRR_D_23_01897
crossref_primary_10_4103_1673_5374_353482
crossref_primary_10_1134_S0026893321040087
crossref_primary_10_1002_adhm_202100805
crossref_primary_10_1016_j_pneurobio_2018_12_007
ContentType Journal Article
Copyright Copyright © 2017 Elsevier Inc. All rights reserved.
Copyright_xml – notice: Copyright © 2017 Elsevier Inc. All rights reserved.
DBID CGR
CUY
CVF
ECM
EIF
NPM
7X8
DOI 10.1016/j.stem.2017.06.011
DatabaseName Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
MEDLINE - Academic
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
MEDLINE - Academic
DatabaseTitleList MEDLINE - Academic
MEDLINE
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: 7X8
  name: MEDLINE - Academic
  url: https://search.proquest.com/medline
  sourceTypes: Aggregation Database
DeliveryMethod no_fulltext_linktorsrc
Discipline Biology
EISSN 1875-9777
ExternalDocumentID 28686866
Genre Journal Article
Review
GroupedDBID ---
--K
0R~
0SF
29B
2WC
4.4
457
4G.
53G
5GY
62-
6I.
6J9
7-5
AACTN
AAEDT
AAEDW
AAFTH
AAKRW
AALRI
AAMRU
AAVLU
AAXUO
ABJNI
ABMAC
ABOCM
ABVKL
ACGFO
ACGFS
ADBBV
ADEZE
ADVLN
AEFWE
AENEX
AEXQZ
AFTJW
AGKMS
AITUG
AKAPO
AKRWK
ALMA_UNASSIGNED_HOLDINGS
AMRAJ
ASPBG
AVWKF
AZFZN
BAWUL
CGR
CS3
CUY
CVF
DIK
DU5
E3Z
EBS
ECM
EIF
EJD
F5P
FCP
FDB
FEDTE
HVGLF
IHE
IXB
JIG
M41
NCXOZ
NPM
O-L
O9-
OK1
P2P
RCE
RIG
ROL
RPZ
SES
SSZ
TR2
ZBA
7X8
AAYWO
ABDGV
ACVFH
ADCNI
AEUPX
AFPUW
AIGII
AKBMS
AKYEP
APXCP
EFKBS
ID FETCH-LOGICAL-c466t-881c5585326476823ef6f02bffe6c6a4e0965ae4292190f2eae1e7d54b96383a2
IEDL.DBID 7X8
ISICitedReferencesCount 126
ISICitedReferencesURI http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000404888100008&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
ISSN 1875-9777
IngestDate Sat Sep 27 23:10:43 EDT 2025
Wed Feb 19 02:42:12 EST 2025
IsDoiOpenAccess false
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 1
Keywords stem cells
brain repair
direct reprogramming
iPSC
neurogenesis
neuron
transdifferentiation
metabolism
conversion
Language English
License Copyright © 2017 Elsevier Inc. All rights reserved.
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c466t-881c5585326476823ef6f02bffe6c6a4e0965ae4292190f2eae1e7d54b96383a2
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
ObjectType-Review-3
content type line 23
OpenAccessLink http://www.cell.com/article/S1934590917302370/pdf
PMID 28686866
PQID 1917364574
PQPubID 23479
ParticipantIDs proquest_miscellaneous_1917364574
pubmed_primary_28686866
PublicationCentury 2000
PublicationDate 2017-07-06
PublicationDateYYYYMMDD 2017-07-06
PublicationDate_xml – month: 07
  year: 2017
  text: 2017-07-06
  day: 06
PublicationDecade 2010
PublicationPlace United States
PublicationPlace_xml – name: United States
PublicationTitle Cell stem cell
PublicationTitleAlternate Cell Stem Cell
PublicationYear 2017
SSID ssj0057107
Score 2.5645058
SecondaryResourceType review_article
Snippet The ability to directly reprogram mature cells to alternative fates challenges concepts of how cell identities are maintained, erased, and acquired. Recent...
SourceID proquest
pubmed
SourceType Aggregation Database
Index Database
StartPage 18
SubjectTerms Animals
Cellular Reprogramming
Cellular Reprogramming Techniques - methods
Humans
Neurons - cytology
Neurons - metabolism
Title Direct Neuronal Reprogramming: Achievements, Hurdles, and New Roads to Success
URI https://www.ncbi.nlm.nih.gov/pubmed/28686866
https://www.proquest.com/docview/1917364574
Volume 21
WOSCitedRecordID wos000404888100008&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
hasFullText
inHoldings 1
isFullTextHit
isPrint
link http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1JS8QwFA7qKHhxX8aNCB4NtmmadLyIiMMctAxuzK1ksuCAtuO0o_jvfUk7ehIEKZQeGijJW77kvX4fQieKShsIE5EwsYIwFQREDiNFHNm3VDJiQ988_nQj0jQZDDr95sCtbNoqZzHRB2pdKHdGfub2Fa5kJtjF-I041ShXXW0kNOZRKwIo46xaDL6rCDFkTy-uApicAM4RzU8zdX-X40l2rV3C83eG4e8Q06ea7up_P3INrTQgE1_WVrGO5ky-gZZq2cnPTZTWcQ57Yg73IqDwuk3rFRLZOb5UzyPjecSr8hT3phP9YuBB5hqGfOC7QuoSVwW-n3q1xS302L1-uOqRRleBKMZ5RZIkVDFsEwC5Mdht0MhYbgM6tNZwxSUzjhFGGidkBXDBUiNNaISO2dB5ayTpNlrIi9zsImx0ILkGv5cxZapjZayFoVQnEZOJiqM2Op5NVAZ264oRMjfFtMx-pqqNdurZzsY1wUZGE-4uvveH0fto2S2i76DlB6hlwWvNIVpU79WonBx5g4B72r_9Ahoovw4
linkProvider ProQuest
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Direct+Neuronal+Reprogramming%3A+Achievements%2C+Hurdles%2C+and+New+Roads+to+Success&rft.jtitle=Cell+stem+cell&rft.au=Gasc%C3%B3n%2C+Sergio&rft.au=Masserdotti%2C+Giacomo&rft.au=Russo%2C+Gianluca+Luigi&rft.au=G%C3%B6tz%2C+Magdalena&rft.date=2017-07-06&rft.eissn=1875-9777&rft.volume=21&rft.issue=1&rft.spage=18&rft_id=info:doi/10.1016%2Fj.stem.2017.06.011&rft_id=info%3Apmid%2F28686866&rft_id=info%3Apmid%2F28686866&rft.externalDocID=28686866
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1875-9777&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1875-9777&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1875-9777&client=summon