Expression of Transforming Growth Factor-beta 1 (TGF-β1) in the Developing Periodontium of Rats

Transforming growth factor-beta 1 (TGF-pl) has been reported to be expressed within several tissue compartments of developing molar crowns and therefore is implicated in tooth development. Additionally, TGF-β1 may also play a crucial role in tissue repair and regeneration. The aim of this study was...

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Published in:Journal of dental research Vol. 77; no. 9; pp. 1708 - 1716
Main Authors: Gao, J., Symons, A.L., Bartold, P.M.
Format: Journal Article
Language:English
Published: Los Angeles, CA SAGE Publications 01.09.1998
SAGE PUBLICATIONS, INC
Subjects:
Aging - metabolism
Animals
Cell Count
developing periodontium
Immunohistochemistry
in situ hybridization
In Situ Hybridization - methods
Molar
Periodontium - cytology
Periodontium - growth & development
Periodontium - metabolism
rat
Rats
Rats, Inbred Lew
RNA, Messenger - metabolism
TGF-β1
Tooth Eruption - physiology
Transforming Growth Factor beta - metabolism
developing periodontium
immunohistochemistry
rat
TGF-pl
in situ hybridization
ISSN:0022-0345, 1544-0591
Online Access:Get full text
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Summary:Transforming growth factor-beta 1 (TGF-pl) has been reported to be expressed within several tissue compartments of developing molar crowns and therefore is implicated in tooth development. Additionally, TGF-β1 may also play a crucial role in tissue repair and regeneration. The aim of this study was to determine the distribution of TGF-β1 in the developing periodontal attachment apparatus (cementum, periodontal ligament, and alveolar bone) in Lewis rats. Animals aged 3, 6, and 12 wks were killed, their mandibles removed, fixed, demineralized, and processed in paraffin. The localization of TGF-β1 in tissues was detected by polyclonal goat antibodies against human TGF-pl by means of immunoperoxidase techniques. TGF-β1 messenger RNA was detected by in situ hybridization with a cocktail oligonucleotide probe. Cell counts were determined for analysis of the percentage of cells stained positive for TGF-β1. Results revealed that TGF-β1 was expressed in the developing alveolar bone, periodontal ligament, and cementum at all stages of tissue development studied. Staining was stronger at sites of cementum and alveolar bone compared with the periodontal ligament. Intensity of the positive staining, based on 3 grades, indicated a similarity between the tissues obtained from different ages, but varied between several cell types. Cementoblasts and osteoblasts stained more strongly than fibroblasts. Large numbers (- 90%) of the osteocytes in developing bone expressed TGF-β1; however, in mature bone, fewer osteocytes stained for TGF-β1. The percentages of positively stained cementoblasts, osteoblasts, and fibroblasts in the periodontal space were greater at the apical portion than at the cervical portion of the root. TGF-β1 mRNA was expressed in osteoblasts, some bone marrow cells, cementoblasts, and fibroblasts. This study indicates that TGF-β1 may play an important role in the modulation of tissue formation and development of the periodontium.
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ISSN:0022-0345
1544-0591
DOI:10.1177/00220345980770090701