Glioblastoma-infiltrated innate immune cells resemble M0 macrophage phenotype

Glioblastomas are highly infiltrated by diverse immune cells, including microglia, macrophages, and myeloid-derived suppressor cells (MDSCs). Understanding the mechanisms by which glioblastoma-associated myeloid cells (GAMs) undergo metamorphosis into tumor-supportive cells, characterizing the heter...

Celý popis

Uložené v:
Podrobná bibliografia
Vydané v:JCI insight Ročník 1; číslo 2
Hlavní autori: Gabrusiewicz, Konrad, Rodriguez, Benjamin, Wei, Jun, Hashimoto, Yuuri, Healy, Luke M., Maiti, Sourindra N., Thomas, Ginu, Zhou, Shouhao, Wang, Qianghu, Elakkad, Ahmed, Liebelt, Brandon D., Yaghi, Nasser K., Ezhilarasan, Ravesanker, Huang, Neal, Weinberg, Jeffrey S., Prabhu, Sujit S., Rao, Ganesh, Sawaya, Raymond, Langford, Lauren A., Bruner, Janet M., Fuller, Gregory N., Bar-Or, Amit, Li, Wei, Colen, Rivka R., Curran, Michael A., Bhat, Krishna P., Antel, Jack P., Cooper, Laurence J., Sulman, Erik P., Heimberger, Amy B.
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: United States American Society for Clinical Investigation 25.02.2016
ISSN:2379-3708, 2379-3708
On-line prístup:Získať plný text
Tagy: Pridať tag
Žiadne tagy, Buďte prvý, kto otaguje tento záznam!
Popis
Shrnutí:Glioblastomas are highly infiltrated by diverse immune cells, including microglia, macrophages, and myeloid-derived suppressor cells (MDSCs). Understanding the mechanisms by which glioblastoma-associated myeloid cells (GAMs) undergo metamorphosis into tumor-supportive cells, characterizing the heterogeneity of immune cell phenotypes within glioblastoma subtypes, and discovering new targets can help the design of new efficient immunotherapies. In this study, we performed a comprehensive battery of immune phenotyping, whole-genome microarray analysis, and microRNA expression profiling of GAMs with matched blood monocytes, healthy donor monocytes, normal brain microglia, nonpolarized M0 macrophages, and polarized M1, M2a, M2c macrophages. Glioblastoma patients had an elevated number of monocytes relative to healthy donors. Among CD11b cells, microglia and MDSCs constituted a higher percentage of GAMs than did macrophages. GAM profiling using flow cytometry studies revealed a continuum between the M1- and M2-like phenotype. Contrary to current dogma, GAMs exhibited distinct immunological functions, with the former aligned close to nonpolarized M0 macrophages.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Authorship note: K. Gabrusiewicz and B. Rodriguez contributed equally to this work.
ISSN:2379-3708
2379-3708
DOI:10.1172/jci.insight.85841