Glioblastoma-infiltrated innate immune cells resemble M0 macrophage phenotype

Glioblastomas are highly infiltrated by diverse immune cells, including microglia, macrophages, and myeloid-derived suppressor cells (MDSCs). Understanding the mechanisms by which glioblastoma-associated myeloid cells (GAMs) undergo metamorphosis into tumor-supportive cells, characterizing the heter...

Celý popis

Uloženo v:

Podrobná bibliografie
Vydáno v:	JCI insight Ročník 1; číslo 2
Hlavní autoři:	Gabrusiewicz, Konrad, Rodriguez, Benjamin, Wei, Jun, Hashimoto, Yuuri, Healy, Luke M., Maiti, Sourindra N., Thomas, Ginu, Zhou, Shouhao, Wang, Qianghu, Elakkad, Ahmed, Liebelt, Brandon D., Yaghi, Nasser K., Ezhilarasan, Ravesanker, Huang, Neal, Weinberg, Jeffrey S., Prabhu, Sujit S., Rao, Ganesh, Sawaya, Raymond, Langford, Lauren A., Bruner, Janet M., Fuller, Gregory N., Bar-Or, Amit, Li, Wei, Colen, Rivka R., Curran, Michael A., Bhat, Krishna P., Antel, Jack P., Cooper, Laurence J., Sulman, Erik P., Heimberger, Amy B.
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	United States American Society for Clinical Investigation 25.02.2016
ISSN:	2379-3708, 2379-3708
On-line přístup:	Získat plný text
Tagy:	Přidat tag Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!

Abstract	Glioblastomas are highly infiltrated by diverse immune cells, including microglia, macrophages, and myeloid-derived suppressor cells (MDSCs). Understanding the mechanisms by which glioblastoma-associated myeloid cells (GAMs) undergo metamorphosis into tumor-supportive cells, characterizing the heterogeneity of immune cell phenotypes within glioblastoma subtypes, and discovering new targets can help the design of new efficient immunotherapies. In this study, we performed a comprehensive battery of immune phenotyping, whole-genome microarray analysis, and microRNA expression profiling of GAMs with matched blood monocytes, healthy donor monocytes, normal brain microglia, nonpolarized M0 macrophages, and polarized M1, M2a, M2c macrophages. Glioblastoma patients had an elevated number of monocytes relative to healthy donors. Among CD11b cells, microglia and MDSCs constituted a higher percentage of GAMs than did macrophages. GAM profiling using flow cytometry studies revealed a continuum between the M1- and M2-like phenotype. Contrary to current dogma, GAMs exhibited distinct immunological functions, with the former aligned close to nonpolarized M0 macrophages.
AbstractList	Glioblastomas are highly infiltrated by diverse immune cells, including microglia, macrophages, and myeloid-derived suppressor cells (MDSCs). Understanding the mechanisms by which glioblastoma-associated myeloid cells (GAMs) undergo metamorphosis into tumor-supportive cells, characterizing the heterogeneity of immune cell phenotypes within glioblastoma subtypes, and discovering new targets can help the design of new efficient immunotherapies. In this study, we performed a comprehensive battery of immune phenotyping, whole-genome microarray analysis, and microRNA expression profiling of GAMs with matched blood monocytes, healthy donor monocytes, normal brain microglia, nonpolarized M0 macrophages, and polarized M1, M2a, M2c macrophages. Glioblastoma patients had an elevated number of monocytes relative to healthy donors. Among CD11b+ cells, microglia and MDSCs constituted a higher percentage of GAMs than did macrophages. GAM profiling using flow cytometry studies revealed a continuum between the M1- and M2-like phenotype. Contrary to current dogma, GAMs exhibited distinct immunological functions, with the former aligned close to nonpolarized M0 macrophages. Glioblastomas are highly infiltrated by diverse immune cells, including microglia, macrophages, and myeloid-derived suppressor cells (MDSCs). Understanding the mechanisms by which glioblastoma-associated myeloid cells (GAMs) undergo metamorphosis into tumor-supportive cells, characterizing the heterogeneity of immune cell phenotypes within glioblastoma subtypes, and discovering new targets can help the design of new efficient immunotherapies. In this study, we performed a comprehensive battery of immune phenotyping, whole-genome microarray analysis, and microRNA expression profiling of GAMs with matched blood monocytes, healthy donor monocytes, normal brain microglia, nonpolarized M0 macrophages, and polarized M1, M2a, M2c macrophages. Glioblastoma patients had an elevated number of monocytes relative to healthy donors. Among CD11b cells, microglia and MDSCs constituted a higher percentage of GAMs than did macrophages. GAM profiling using flow cytometry studies revealed a continuum between the M1- and M2-like phenotype. Contrary to current dogma, GAMs exhibited distinct immunological functions, with the former aligned close to nonpolarized M0 macrophages.
Author	Ezhilarasan, Ravesanker Curran, Michael A. Weinberg, Jeffrey S. Thomas, Ginu Bruner, Janet M. Li, Wei Cooper, Laurence J. Yaghi, Nasser K. Heimberger, Amy B. Antel, Jack P. Wei, Jun Gabrusiewicz, Konrad Sulman, Erik P. Rao, Ganesh Langford, Lauren A. Fuller, Gregory N. Wang, Qianghu Maiti, Sourindra N. Prabhu, Sujit S. Bar-Or, Amit Colen, Rivka R. Elakkad, Ahmed Liebelt, Brandon D. Hashimoto, Yuuri Huang, Neal Sawaya, Raymond Healy, Luke M. Rodriguez, Benjamin Bhat, Krishna P. Zhou, Shouhao
AuthorAffiliation	1 Department of Neurosurgery, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA 3 Neuroimmunology Unit, Department of Neurology and Neurosurgery, Montreal Neurological Institute and Hospital, McGill University, Montreal, Quebec, Canada 9 Department of Neuropathology 10 Department of Immunology, and 7 Department of Bioinformatics and Computational Biology 8 Department of Radiation Oncology 6 Department of Biostatistics 11 Department of Translational Molecular Pathology, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA 2 Division of Biostatistics, Dan L. Duncan Cancer Center and Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas, USA 5 Department of Diagnostic Radiology 4 Division of Pediatrics
AuthorAffiliation_xml	– name: 5 Department of Diagnostic Radiology – name: 8 Department of Radiation Oncology – name: 10 Department of Immunology, and – name: 2 Division of Biostatistics, Dan L. Duncan Cancer Center and Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas, USA – name: 3 Neuroimmunology Unit, Department of Neurology and Neurosurgery, Montreal Neurological Institute and Hospital, McGill University, Montreal, Quebec, Canada – name: 4 Division of Pediatrics – name: 6 Department of Biostatistics – name: 7 Department of Bioinformatics and Computational Biology – name: 9 Department of Neuropathology – name: 1 Department of Neurosurgery, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA – name: 11 Department of Translational Molecular Pathology, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA
Author_xml	– sequence: 1 givenname: Konrad surname: Gabrusiewicz fullname: Gabrusiewicz, Konrad – sequence: 2 givenname: Benjamin surname: Rodriguez fullname: Rodriguez, Benjamin – sequence: 3 givenname: Jun surname: Wei fullname: Wei, Jun – sequence: 4 givenname: Yuuri surname: Hashimoto fullname: Hashimoto, Yuuri – sequence: 5 givenname: Luke M. surname: Healy fullname: Healy, Luke M. – sequence: 6 givenname: Sourindra N. surname: Maiti fullname: Maiti, Sourindra N. – sequence: 7 givenname: Ginu surname: Thomas fullname: Thomas, Ginu – sequence: 8 givenname: Shouhao surname: Zhou fullname: Zhou, Shouhao – sequence: 9 givenname: Qianghu surname: Wang fullname: Wang, Qianghu – sequence: 10 givenname: Ahmed surname: Elakkad fullname: Elakkad, Ahmed – sequence: 11 givenname: Brandon D. surname: Liebelt fullname: Liebelt, Brandon D. – sequence: 12 givenname: Nasser K. surname: Yaghi fullname: Yaghi, Nasser K. – sequence: 13 givenname: Ravesanker surname: Ezhilarasan fullname: Ezhilarasan, Ravesanker – sequence: 14 givenname: Neal surname: Huang fullname: Huang, Neal – sequence: 15 givenname: Jeffrey S. surname: Weinberg fullname: Weinberg, Jeffrey S. – sequence: 16 givenname: Sujit S. surname: Prabhu fullname: Prabhu, Sujit S. – sequence: 17 givenname: Ganesh surname: Rao fullname: Rao, Ganesh – sequence: 18 givenname: Raymond surname: Sawaya fullname: Sawaya, Raymond – sequence: 19 givenname: Lauren A. surname: Langford fullname: Langford, Lauren A. – sequence: 20 givenname: Janet M. surname: Bruner fullname: Bruner, Janet M. – sequence: 21 givenname: Gregory N. surname: Fuller fullname: Fuller, Gregory N. – sequence: 22 givenname: Amit surname: Bar-Or fullname: Bar-Or, Amit – sequence: 23 givenname: Wei surname: Li fullname: Li, Wei – sequence: 24 givenname: Rivka R. surname: Colen fullname: Colen, Rivka R. – sequence: 25 givenname: Michael A. surname: Curran fullname: Curran, Michael A. – sequence: 26 givenname: Krishna P. surname: Bhat fullname: Bhat, Krishna P. – sequence: 27 givenname: Jack P. surname: Antel fullname: Antel, Jack P. – sequence: 28 givenname: Laurence J. surname: Cooper fullname: Cooper, Laurence J. – sequence: 29 givenname: Erik P. surname: Sulman fullname: Sulman, Erik P. – sequence: 30 givenname: Amy B. surname: Heimberger fullname: Heimberger, Amy B.
BackLink	https://www.ncbi.nlm.nih.gov/pubmed/26973881$$D View this record in MEDLINE/PubMed
BookMark	eNp1UctKxDAUDaL4nA9wI1266ZiknTw2goiOguJG1yFNb2ciaVKbjODfm9FRVHB1LtzzSO45QNs-eEDomOApIZyePRs7tT7axTJNxUzUZAvt04rLsuJYbP-Y99AkxmeMMeE1xTOxi_Yok7wSguyj-7mzoXE6ptDr0vrOujTqBG1hvc9Y2L5feSgMOBeLESL0jYPiHhe9NmMYlnoBxbAEH9LbAEdop9MuwmSDh-jp-urx8qa8e5jfXl7claZms1SCJJjhqsvYMtZUsmkMk0JqLQWlnaaklUZ0EhomKOfE1MAZSMNaDSCAVYfo_NN3WDU9tAZ8frRTw2h7Pb6poK36vfF2qRbhVdVc1JSRbHC6MRjDywpiUr2N6z9qD2EVFRGUMUZ4hTP15GfWd8jXDTOBfxLyPWIcoVPGJp1sWEdbpwhW68JULkxtClMfhWUl-aP8Mv9f8w4sep89
CitedBy_id	crossref_primary_10_1097_WCO_0000000000000746 crossref_primary_10_1038_s41388_017_0024_z crossref_primary_10_3389_fonc_2022_819599 crossref_primary_10_1038_s41598_019_54500_8 crossref_primary_10_1186_s12885_023_11057_0 crossref_primary_10_1111_jcmm_16563 crossref_primary_10_3390_cells13060535 crossref_primary_10_1038_s41467_021_22088_1 crossref_primary_10_1038_s41598_023_32158_7 crossref_primary_10_3390_cells10040893 crossref_primary_10_3390_ijms21030888 crossref_primary_10_3389_fncel_2018_00235 crossref_primary_10_1186_s13014_022_02038_x crossref_primary_10_1158_1078_0432_CCR_19_3104 crossref_primary_10_1016_j_ebiom_2023_104963 crossref_primary_10_1038_s41598_022_10114_1 crossref_primary_10_1016_j_addr_2017_06_006 crossref_primary_10_1016_j_cyto_2022_156059 crossref_primary_10_1186_s13046_025_03393_9 crossref_primary_10_1186_s12974_022_02630_8 crossref_primary_10_3389_fimmu_2020_01842 crossref_primary_10_18632_oncotarget_24102 crossref_primary_10_1111_jcmm_14277 crossref_primary_10_1186_s12859_022_05091_1 crossref_primary_10_1186_s12944_022_01759_y crossref_primary_10_3389_fimmu_2021_809826 crossref_primary_10_1177_15593258231173199 crossref_primary_10_3390_ijms21218412 crossref_primary_10_1007_s11060_020_03483_y crossref_primary_10_1186_s12885_022_09872_y crossref_primary_10_1016_j_it_2021_02_004 crossref_primary_10_1186_s40478_025_02057_1 crossref_primary_10_1002_1878_0261_13657 crossref_primary_10_1073_pnas_1902366116 crossref_primary_10_3389_fonc_2022_822085 crossref_primary_10_1016_j_celrep_2024_115149 crossref_primary_10_3390_antiox11112123 crossref_primary_10_1016_j_critrevonc_2022_103641 crossref_primary_10_1136_jitc_2021_002644 crossref_primary_10_1002_glia_24065 crossref_primary_10_1186_s13046_022_02251_2 crossref_primary_10_3389_fimmu_2023_1123853 crossref_primary_10_1186_s12885_024_11875_w crossref_primary_10_3389_fimmu_2024_1326753 crossref_primary_10_1186_s13046_019_1228_6 crossref_primary_10_1038_s41467_023_37361_8 crossref_primary_10_1038_s41598_023_42848_x crossref_primary_10_1111_nan_13012 crossref_primary_10_1016_j_ccell_2016_11_015 crossref_primary_10_3389_fonc_2020_01399 crossref_primary_10_3389_fonc_2022_1022716 crossref_primary_10_1038_s41598_023_45566_6 crossref_primary_10_1080_2162402X_2017_1412909 crossref_primary_10_3389_fimmu_2024_1326757 crossref_primary_10_1158_2767_9764_CRC_23_0189 crossref_primary_10_3390_biomedicines10020292 crossref_primary_10_1158_1078_0432_CCR_20_1135 crossref_primary_10_3389_fimmu_2021_701383 crossref_primary_10_3389_fimmu_2022_1024921 crossref_primary_10_3389_fimmu_2022_1038096 crossref_primary_10_1038_s41388_021_02010_1 crossref_primary_10_3390_cancers12123813 crossref_primary_10_3390_brainsci12040505 crossref_primary_10_3390_cells10030607 crossref_primary_10_1002_glia_23883 crossref_primary_10_1016_j_intimp_2025_115064 crossref_primary_10_1038_s41598_017_17752_w crossref_primary_10_1038_s41467_018_08271_x crossref_primary_10_3390_ijms24065115 crossref_primary_10_3390_cells8121477 crossref_primary_10_1186_s12885_019_6502_7 crossref_primary_10_3390_ijms22157981 crossref_primary_10_1158_1078_0432_CCR_24_2830 crossref_primary_10_1038_s41698_025_00920_x crossref_primary_10_3390_cancers15235530 crossref_primary_10_3389_fonc_2023_1196448 crossref_primary_10_3390_ijms241210032 crossref_primary_10_3389_fimmu_2023_1284118 crossref_primary_10_3390_ijms232415612 crossref_primary_10_1038_s41598_025_06909_7 crossref_primary_10_1172_JCI121266 crossref_primary_10_1158_2159_8290_CD_21_0291 crossref_primary_10_1016_j_canlet_2018_07_034 crossref_primary_10_1016_j_ccell_2019_05_003 crossref_primary_10_1016_j_tcb_2021_06_008 crossref_primary_10_1080_14656566_2019_1621840 crossref_primary_10_1093_neuonc_noaa277 crossref_primary_10_3390_ijms21030689 crossref_primary_10_1002_cti2_1050 crossref_primary_10_1042_BST20180444 crossref_primary_10_3390_cancers13153686 crossref_primary_10_1158_1078_0432_CCR_21_2347 crossref_primary_10_3389_fimmu_2021_679954 crossref_primary_10_1007_s43939_024_00133_2 crossref_primary_10_3389_fcimb_2023_1141034 crossref_primary_10_3390_cancers11010005 crossref_primary_10_1002_glia_23984 crossref_primary_10_1038_onc_2017_145 crossref_primary_10_1172_JCI98060 crossref_primary_10_1007_s00262_019_02423_8 crossref_primary_10_1080_14728222_2020_1762568 crossref_primary_10_3390_cancers13174255 crossref_primary_10_3390_ijms222413382 crossref_primary_10_3389_fonc_2023_1168454 crossref_primary_10_3390_life12081225 crossref_primary_10_3390_ijms21207619 crossref_primary_10_2147_OTT_S483536 crossref_primary_10_3390_cimb46090588 crossref_primary_10_1111_imr_12529 crossref_primary_10_1016_j_neulet_2017_02_076 crossref_primary_10_1016_j_wneu_2024_06_142 crossref_primary_10_3390_ijms21072263 crossref_primary_10_3389_fgene_2021_718717 crossref_primary_10_3389_fonc_2017_00251 crossref_primary_10_1186_s12967_024_05149_z crossref_primary_10_1007_s11060_017_2508_7 crossref_primary_10_1186_s13046_021_01997_5 crossref_primary_10_3389_fonc_2016_00256 crossref_primary_10_1158_1078_0432_CCR_20_0536 crossref_primary_10_1186_s40478_021_01124_7 crossref_primary_10_3390_cells10082032 crossref_primary_10_1186_s12885_022_09328_3 crossref_primary_10_1007_s12672_022_00514_0 crossref_primary_10_1111_nan_12489 crossref_primary_10_1016_j_cytogfr_2024_10_009 crossref_primary_10_4049_jimmunol_2000792 crossref_primary_10_1093_neuonc_noaa259 crossref_primary_10_1016_j_lfs_2024_123089 crossref_primary_10_1371_journal_pone_0185376 crossref_primary_10_1007_s11060_019_03230_y crossref_primary_10_15252_embj_2019103790 crossref_primary_10_1002_1878_0261_13287 crossref_primary_10_3390_cancers14225665 crossref_primary_10_3389_fimmu_2022_1089266 crossref_primary_10_1007_s11060_017_2622_6 crossref_primary_10_1038_s41417_021_00320_w crossref_primary_10_1038_s41467_020_18070_y crossref_primary_10_1093_neuonc_noae193 crossref_primary_10_1111_bpa_12927 crossref_primary_10_2217_imt_2017_0122 crossref_primary_10_3389_fgene_2021_657051 crossref_primary_10_3390_cancers15174376 crossref_primary_10_3390_ijms24032020 crossref_primary_10_1007_s11060_018_2761_4 crossref_primary_10_1016_j_bcp_2025_116898 crossref_primary_10_1093_neuonc_noaa023 crossref_primary_10_3389_fonc_2018_00578 crossref_primary_10_1038_s41598_020_65320_6 crossref_primary_10_2217_mmt_2020_0019 crossref_primary_10_1136_jitc_2025_012009 crossref_primary_10_1038_s41467_018_03050_0 crossref_primary_10_1080_14737159_2021_1900735 crossref_primary_10_3390_ijms21061950 crossref_primary_10_3389_fonc_2022_878849 crossref_primary_10_3390_diagnostics10050256 crossref_primary_10_1016_j_wneu_2021_06_107 crossref_primary_10_3390_cells10123529 crossref_primary_10_3389_fimmu_2024_1423232 crossref_primary_10_1111_jcmm_15758 crossref_primary_10_3390_ijms22063027 crossref_primary_10_1016_j_xpro_2024_103079 crossref_primary_10_1038_labinvest_2017_19 crossref_primary_10_3389_fonc_2021_665235 crossref_primary_10_3390_ijms19103054 crossref_primary_10_3389_fphar_2019_00506 crossref_primary_10_1093_neuonc_noaa082 crossref_primary_10_3389_fncel_2021_716947 crossref_primary_10_1038_s41571_022_00620_6 crossref_primary_10_1186_s13046_024_03028_5 crossref_primary_10_1007_s11307_019_01427_1 crossref_primary_10_1038_s41582_019_0220_2 crossref_primary_10_1016_j_lfs_2021_119505 crossref_primary_10_3389_fimmu_2024_1393173 crossref_primary_10_1038_s43018_022_00475_x crossref_primary_10_1111_nan_12428 crossref_primary_10_3390_cancers14051319 crossref_primary_10_3390_ijms19102879 crossref_primary_10_1016_j_mam_2022_101167 crossref_primary_10_3389_fneur_2022_1047162 crossref_primary_10_1038_s41568_021_00397_3 crossref_primary_10_1080_2162402X_2020_1779990 crossref_primary_10_1136_jitc_2021_003246 crossref_primary_10_3390_brainsci12020181 crossref_primary_10_1038_s41419_025_07819_2 crossref_primary_10_3389_fimmu_2024_1438044 crossref_primary_10_1007_s11523_019_00655_3 crossref_primary_10_3390_cancers14215460 crossref_primary_10_3390_cancers12061622 crossref_primary_10_1111_cas_16128 crossref_primary_10_1093_neuonc_noab182 crossref_primary_10_3389_fonc_2023_1274783 crossref_primary_10_1016_j_jneuroim_2019_04_010 crossref_primary_10_3390_cells10092264 crossref_primary_10_1016_j_canlet_2023_216380 crossref_primary_10_1016_j_ccell_2017_06_003 crossref_primary_10_3389_fonc_2021_770561 crossref_primary_10_1038_s41467_023_36878_2 crossref_primary_10_3390_cells9020337 crossref_primary_10_3389_fonc_2021_682129 crossref_primary_10_3390_cells10071783 crossref_primary_10_3233_CBM_220259 crossref_primary_10_1080_2162402X_2021_1957215 crossref_primary_10_3389_fmed_2023_1083242 crossref_primary_10_3390_cancers15133480 crossref_primary_10_1007_s00262_020_02836_w crossref_primary_10_1007_s11033_023_09196_5 crossref_primary_10_1016_j_ccell_2017_02_009 crossref_primary_10_3390_brainsci12070893 crossref_primary_10_1016_j_compbiomed_2025_109730 crossref_primary_10_1080_2162402X_2018_1561106 crossref_primary_10_1080_2162402X_2018_1431083 crossref_primary_10_1186_s12967_025_06599_9 crossref_primary_10_3389_fncel_2020_558381 crossref_primary_10_1016_j_biopha_2023_114646 crossref_primary_10_1002_glia_23014 crossref_primary_10_1038_s41590_022_01267_2 crossref_primary_10_3389_fonc_2021_719091 crossref_primary_10_1155_2022_6711085 crossref_primary_10_3389_fonc_2017_00143 crossref_primary_10_1186_s40478_023_01605_x crossref_primary_10_1093_neuonc_noz185 crossref_primary_10_3390_ijms232214164 crossref_primary_10_1007_s00432_021_03701_8 crossref_primary_10_1111_cei_13403 crossref_primary_10_1002_dvdy_24662 crossref_primary_10_3389_fcell_2021_619396 crossref_primary_10_3390_biomedicines8060151 crossref_primary_10_3390_biology12121528 crossref_primary_10_1002_smll_202302613 crossref_primary_10_3389_fonc_2021_729103 crossref_primary_10_1111_jcmm_18208 crossref_primary_10_3390_app14041391 crossref_primary_10_3390_ijms21165647 crossref_primary_10_1038_s44319_024_00117_6 crossref_primary_10_1016_j_jneuroim_2022_578009 crossref_primary_10_1038_s41419_023_05753_9 crossref_primary_10_1016_j_lfs_2024_123150 crossref_primary_10_1186_s12964_024_01523_y crossref_primary_10_1038_s41416_021_01515_6 crossref_primary_10_1111_bpa_12690 crossref_primary_10_1186_s40478_019_0704_8 crossref_primary_10_1093_neuonc_now280 crossref_primary_10_3389_fimmu_2023_994698 crossref_primary_10_1186_s12951_020_00589_3 crossref_primary_10_3390_cancers12123664 crossref_primary_10_1007_s12094_024_03395_7 crossref_primary_10_3389_fimmu_2019_02294 crossref_primary_10_1038_s41416_022_01864_w crossref_primary_10_1007_s00335_018_9761_8 crossref_primary_10_3390_ijms22073301 crossref_primary_10_1158_1078_0432_CCR_22_3273 crossref_primary_10_1016_j_cell_2020_05_007 crossref_primary_10_1038_s41571_021_00518_9 crossref_primary_10_3390_cancers14133249 crossref_primary_10_1177_1010428317695528 crossref_primary_10_1016_j_bbih_2023_100688 crossref_primary_10_1186_s13578_020_00454_8 crossref_primary_10_1038_s41598_024_64207_0 crossref_primary_10_3389_fimmu_2017_01349 crossref_primary_10_1186_s12964_019_0492_6 crossref_primary_10_3389_fcell_2021_695325 crossref_primary_10_1111_jcmm_18260 crossref_primary_10_3389_fimmu_2020_588552 crossref_primary_10_1007_s11060_018_03005_x crossref_primary_10_1186_s40478_019_0803_6 crossref_primary_10_1038_s41389_019_0146_y crossref_primary_10_1172_JCI163451 crossref_primary_10_1158_1078_0432_CCR_19_4110 crossref_primary_10_1038_s41598_022_08618_x crossref_primary_10_5493_wjem_v15_i2_102285 crossref_primary_10_1002_advs_202002015 crossref_primary_10_1007_s13402_020_00561_1 crossref_primary_10_1016_j_jneuroim_2021_577633 crossref_primary_10_1186_s12885_021_08825_1 crossref_primary_10_1038_s41419_021_04427_8 crossref_primary_10_1080_14737175_2018_1510321 crossref_primary_10_3389_fimmu_2018_01329 crossref_primary_10_3390_cancers17101631 crossref_primary_10_3390_ijms22115775 crossref_primary_10_1155_2020_8844313 crossref_primary_10_3390_cancers17091547 crossref_primary_10_1093_neuonc_now251 crossref_primary_10_3389_fmats_2018_00027 crossref_primary_10_3390_ijms21217955 crossref_primary_10_1016_j_heliyon_2023_e19502 crossref_primary_10_1016_j_imbio_2019_10_002 crossref_primary_10_1002_glia_23686 crossref_primary_10_1016_j_heliyon_2024_e24729 crossref_primary_10_2147_OTT_S215997 crossref_primary_10_1007_s12026_022_09260_5 crossref_primary_10_1007_s00262_018_2214_0 crossref_primary_10_1158_2159_8290_CD_22_0196 crossref_primary_10_1016_j_jneumeth_2018_06_008 crossref_primary_10_1186_s12885_023_10897_0 crossref_primary_10_1186_s40478_022_01323_w crossref_primary_10_1016_j_ccell_2021_05_002 crossref_primary_10_1097_MD_0000000000033935 crossref_primary_10_1186_s40170_023_00325_z crossref_primary_10_3390_cells13100808 crossref_primary_10_1186_s40478_021_01156_z crossref_primary_10_3390_cells10092485 crossref_primary_10_1186_s40425_019_0536_x crossref_primary_10_1093_neuonc_noad257 crossref_primary_10_1089_ars_2022_0187 crossref_primary_10_1080_2162402X_2024_2425124 crossref_primary_10_3389_fimmu_2020_580934 crossref_primary_10_1172_JCI96268 crossref_primary_10_1111_nan_12772 crossref_primary_10_1155_2022_3623591 crossref_primary_10_1007_s00210_024_03559_w
Cites_doi	10.1371/journal.pone.0023902 10.1007/s11060-014-1366-9 10.18632/oncotarg.6662 10.1093/jnci/dju162 10.1007/BF00294647 10.1016/j.redox.2013.12.026 10.1371/journal.pone.0043339 10.1046/j.1365-2990.1998.00120.x 10.3171/2008.7.JNS08475 10.1371/journal.pone.0119751 10.18632/oncotarget.5310 10.1371/journal.pone.0045466 10.1093/neuonc/nop013 10.1016/0167-5699(93)90212-4 10.1002/glia.10147 10.1016/j.it.2004.09.015 10.1007/s004010050520 10.1007/s11033-013-2567-6 10.1002/glia.22298 10.1158/0008-5472.CAN-12-4124 10.1158/0008-5472.CAN-10-3055 10.1002/glia.22370 10.1073/pnas.89.13.6065 10.1007/s004010051093 10.1215/15228517-2006-008 10.1016/j.ccr.2013.08.001 10.1093/neuonc/nor042 10.1002/pmic.201400511 10.1093/humrep/dei320 10.1016/j.neo.2015.03.003 10.1189/jlb.0907611 10.1186/1479-5876-9-90 10.1073/pnas.0509703103 10.1042/BSR20150088 10.2741/2692 10.1016/j.it.2013.02.003 10.1093/neuonc/nov280 10.1182/blood-2011-02-339911 10.1038/sj.bjc.6602702 10.1093/jnci/djv137 10.1002/glia.22510 10.1172/JCI8218 10.4049/jimmunol.177.10.7303 10.1002/ijc.28426 10.1016/j.jneuroim.2010.03.011 10.1158/2326-6066.CIR-13-0028 10.1016/j.ccr.2009.12.020 10.1038/nrc1256 10.1371/journal.pone.0116644 10.1158/1078-0432.CCR-10-1693 10.1158/0008-5472.CAN-12-4318 10.1152/physrev.00011.2010 10.1002/path.2370 10.1016/j.semcancer.2008.03.004 10.1097/NEN.0000000000000183 10.1186/1479-5876-12-7 10.1111/sji.12075 10.1158/1078-0432.CCR-12-1940 10.3892/or.2014.3214 10.4049/jimmunol.1201449 10.1093/neuonc/noq082 10.1186/1479-5876-4-15 10.1007/s12079-012-0168-0 10.1186/1742-2094-9-147 10.1158/1055-9965.EPI-09-1077 10.1073/pnas.88.16.7438 10.1093/neuonc/nos262 10.1038/nature11862 10.1038/nri3175
ContentType	Journal Article
Copyright	Copyright © 2016, American Society for Clinical Investigation 2016 American Society for Clinical Investigation
Copyright_xml	– notice: Copyright © 2016, American Society for Clinical Investigation 2016 American Society for Clinical Investigation
DBID	AAYXX CITATION NPM 7X8 5PM
DOI	10.1172/jci.insight.85841
DatabaseName	CrossRef PubMed MEDLINE - Academic PubMed Central (Full Participant titles)
DatabaseTitle	CrossRef PubMed MEDLINE - Academic
DatabaseTitleList	MEDLINE - Academic PubMed
Database_xml	– sequence: 1 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: 7X8 name: MEDLINE - Academic url: https://search.proquest.com/medline sourceTypes: Aggregation Database
DeliveryMethod	fulltext_linktorsrc
EISSN	2379-3708
ExternalDocumentID	PMC4784261 26973881 10_1172_jci_insight_85841
Genre	Journal Article
GrantInformation_xml	– fundername: NCI NIH HHS grantid: R01 CA120813 – fundername: NCI NIH HHS grantid: P30 CA016672 – fundername: NCI NIH HHS grantid: P50 CA093459 – fundername: NCI NIH HHS grantid: R01 CA193466 – fundername: NCI NIH HHS grantid: P50 CA127001 – fundername: NHGRI NIH HHS grantid: R01 HG007538
GroupedDBID	53G AAFWJ AAYXX ADBBV AFPKN ALMA_UNASSIGNED_HOLDINGS AOIJS CCPQU CITATION GROUPED_DOAJ HYE M~E OK1 RPM NPM 7X8 5PM
ID	FETCH-LOGICAL-c465t-e910603fe91d66b39bbc6989aa9822fa21d9c8f9eb682771c4e76e9c6daee8e63
ISICitedReferencesCount	368
ISICitedReferencesURI	http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000387103700007&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
ISSN	2379-3708
IngestDate	Thu Nov 27 06:11:27 EST 2025 Thu Jul 10 23:42:13 EDT 2025 Thu Jan 02 23:02:40 EST 2025 Sat Nov 29 04:17:18 EST 2025 Tue Nov 18 21:20:00 EST 2025
IsDoiOpenAccess	false
IsOpenAccess	true
IsPeerReviewed	true
IsScholarly	true
Issue	2
Language	English
LinkModel	OpenURL
MergedId	FETCHMERGED-LOGICAL-c465t-e910603fe91d66b39bbc6989aa9822fa21d9c8f9eb682771c4e76e9c6daee8e63
Notes	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Authorship note: K. Gabrusiewicz and B. Rodriguez contributed equally to this work.
ORCID	0000-0003-0547-1242 0000-0002-8124-5047
OpenAccessLink	http://insight.jci.org/articles/view/85841/files/pdf
PMID	26973881
PQID	1826661730
PQPubID	23479
ParticipantIDs	pubmedcentral_primary_oai_pubmedcentral_nih_gov_4784261 proquest_miscellaneous_1826661730 pubmed_primary_26973881 crossref_citationtrail_10_1172_jci_insight_85841 crossref_primary_10_1172_jci_insight_85841
PublicationCentury	2000
PublicationDate	2016-02-25
PublicationDateYYYYMMDD	2016-02-25
PublicationDate_xml	– month: 02 year: 2016 text: 2016-02-25 day: 25
PublicationDecade	2010
PublicationPlace	United States
PublicationPlace_xml	– name: United States
PublicationTitle	JCI insight
PublicationTitleAlternate	JCI Insight
PublicationYear	2016
Publisher	American Society for Clinical Investigation
Publisher_xml	– name: American Society for Clinical Investigation
References	B20 B64 B21 B65 B66 Badie (B1) 2000; 46 B67 B24 B68 B25 Nikodemova (B71) 2012; 9 B26 B27 B28 B29 Dolmans (B69) 2006; 21 Fu (B44) 2001; 7 Hussain (B70) 2006; 4 B72 B73 B30 B31 B33 B34 B35 Mieczkowski (B41) 2015; 6 B36 B37 B38 B39 Wierzba-Bobrowicz (B5) 1994; 32 B2 B3 B4 B6 Wang (B22) 2014; 12 B7 B8 Lechner (B32) 2011; 9 B9 Hattermann (B40) 2014; 32 B42 B43 B45 B46 B47 B48 B49 Hao (B23) 2013; 3 B50 B51 B52 B53 B10 B54 B11 B55 B12 B56 B13 B57 B14 B58 B15 B59 B16 B17 B18 B19 B60 B61 B62 B63
References_xml	– ident: B26 doi: 10.1371/journal.pone.0023902 – ident: B63 doi: 10.1007/s11060-014-1366-9 – ident: B30 doi: 10.18632/oncotarg.6662 – volume: 32 start-page: 251 issue: 4 year: 1994 ident: B5 article-title: Reaction of microglial cells in human astrocytomas (preliminary report) publication-title: Folia Neuropathol – ident: B66 doi: 10.1093/jnci/dju162 – ident: B4 doi: 10.1007/BF00294647 – ident: B51 doi: 10.1016/j.redox.2013.12.026 – ident: B55 doi: 10.1371/journal.pone.0043339 – ident: B42 doi: 10.1046/j.1365-2990.1998.00120.x – ident: B27 doi: 10.3171/2008.7.JNS08475 – ident: B37 doi: 10.1371/journal.pone.0119751 – volume: 6 start-page: 33077 issue: 32 year: 2015 ident: B41 article-title: Down-regulation of IKKbeta expression in glioma-infiltrating microglia/macrophages is associated with defective inflammatory/immune gene responses in glioblastoma publication-title: Oncotarget doi: 10.18632/oncotarget.5310 – ident: B49 doi: 10.1371/journal.pone.0045466 – ident: B54 doi: 10.1093/neuonc/nop013 – ident: B68 doi: 10.1016/0167-5699(93)90212-4 – ident: B6 doi: 10.1002/glia.10147 – ident: B16 doi: 10.1016/j.it.2004.09.015 – ident: B3 doi: 10.1007/s004010050520 – ident: B64 doi: 10.1007/s11033-013-2567-6 – ident: B72 doi: 10.1002/glia.22298 – ident: B46 doi: 10.1158/0008-5472.CAN-12-4124 – volume: 3 start-page: 239 issue: 3 year: 2013 ident: B23 article-title: Detection of immune suppressive neutrophils in peripheral blood samples of cancer patients publication-title: Am J Blood Res – ident: B45 doi: 10.1158/0008-5472.CAN-10-3055 – ident: B73 doi: 10.1002/glia.22370 – ident: B39 doi: 10.1073/pnas.89.13.6065 – ident: B62 doi: 10.1007/s004010051093 – ident: B10 doi: 10.1215/15228517-2006-008 – ident: B53 doi: 10.1016/j.ccr.2013.08.001 – ident: B21 doi: 10.1093/neuonc/nor042 – ident: B25 doi: 10.1002/pmic.201400511 – volume: 21 start-page: 413 issue: 2 year: 2006 ident: B69 article-title: Evaluation of Liberase, a purified enzyme blend, for the isolation of human primordial and primary ovarian follicles publication-title: Hum Reprod doi: 10.1093/humrep/dei320 – ident: B19 doi: 10.1016/j.neo.2015.03.003 – ident: B12 doi: 10.1189/jlb.0907611 – volume: 9 year: 2011 ident: B32 article-title: Functional characterization of human Cd33+ and Cd11b+ myeloid-derived suppressor cell subsets induced from peripheral blood mononuclear cells co-cultured with a diverse set of human tumor cell lines publication-title: J Transl Med doi: 10.1186/1479-5876-9-90 – ident: B59 doi: 10.1073/pnas.0509703103 – ident: B65 doi: 10.1042/BSR20150088 – ident: B14 doi: 10.2741/2692 – ident: B38 doi: 10.1016/j.it.2013.02.003 – ident: B43 doi: 10.1093/neuonc/nov280 – volume: 46 start-page: 957 issue: 4 year: 2000 ident: B1 article-title: Flow cytometric characterization of tumor-associated macrophages in experimental gliomas publication-title: Neurosurgery – ident: B48 doi: 10.1182/blood-2011-02-339911 – ident: B61 doi: 10.1038/sj.bjc.6602702 – ident: B52 doi: 10.1093/jnci/djv137 – ident: B24 doi: 10.1002/glia.22510 – ident: B60 doi: 10.1172/JCI8218 – ident: B36 doi: 10.4049/jimmunol.177.10.7303 – ident: B47 doi: 10.1002/ijc.28426 – ident: B13 doi: 10.1016/j.jneuroim.2010.03.011 – ident: B33 doi: 10.1158/2326-6066.CIR-13-0028 – ident: B34 doi: 10.1016/j.ccr.2009.12.020 – ident: B15 doi: 10.1038/nrc1256 – ident: B29 doi: 10.1371/journal.pone.0116644 – ident: B9 doi: 10.1158/1078-0432.CCR-10-1693 – ident: B67 doi: 10.1158/0008-5472.CAN-12-4318 – ident: B2 doi: 10.1152/physrev.00011.2010 – ident: B17 doi: 10.1002/path.2370 – ident: B7 doi: 10.1016/j.semcancer.2008.03.004 – ident: B20 doi: 10.1097/NEN.0000000000000183 – volume: 12 year: 2014 ident: B22 article-title: The clinical significance of tumor-infiltrating neutrophils and neutrophil-to-CD8+ lymphocyte ratio in patients with resectable esophageal squamous cell carcinoma publication-title: J Transl Med doi: 10.1186/1479-5876-12-7 – volume: 7 start-page: 4182 issue: 12 year: 2001 ident: B44 article-title: Identification of a novel membrane protein, HP59, with therapeutic potential as a target of tumor angiogenesis publication-title: Clin Cancer Res – ident: B58 doi: 10.1111/sji.12075 – ident: B18 doi: 10.1158/1078-0432.CCR-12-1940 – volume: 32 start-page: 270 issue: 1 year: 2014 ident: B40 article-title: Chemokine expression profile of freshly isolated human glioblastoma-associated macrophages/microglia publication-title: Oncol Rep doi: 10.3892/or.2014.3214 – ident: B31 doi: 10.4049/jimmunol.1201449 – ident: B8 doi: 10.1093/neuonc/noq082 – volume: 4 issue: 1 year: 2006 ident: B70 article-title: Innate immune functions of microglia isolated from human glioma patients publication-title: J Transl Med doi: 10.1186/1479-5876-4-15 – ident: B35 doi: 10.1007/s12079-012-0168-0 – volume: 9 year: 2012 ident: B71 article-title: Efficient isolation of live microglia with preserved phenotypes from adult mouse brain publication-title: J Neuroinflammation doi: 10.1186/1742-2094-9-147 – ident: B57 doi: 10.1158/1055-9965.EPI-09-1077 – ident: B28 doi: 10.1073/pnas.88.16.7438 – ident: B56 doi: 10.1093/neuonc/nos262 – ident: B50 doi: 10.1038/nature11862 – ident: B11 doi: 10.1038/nri3175
SSID	ssj0001742058
Score	2.5000532
Snippet	Glioblastomas are highly infiltrated by diverse immune cells, including microglia, macrophages, and myeloid-derived suppressor cells (MDSCs). Understanding the...
SourceID	pubmedcentral proquest pubmed crossref
SourceType	Open Access Repository Aggregation Database Index Database Enrichment Source
Title	Glioblastoma-infiltrated innate immune cells resemble M0 macrophage phenotype
URI	https://www.ncbi.nlm.nih.gov/pubmed/26973881 https://www.proquest.com/docview/1826661730 https://pubmed.ncbi.nlm.nih.gov/PMC4784261
Volume	1
WOSCitedRecordID	wos000387103700007&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
hasFullText	1
inHoldings	1
isFullTextHit
isPrint
journalDatabaseRights	– providerCode: PRVHPJ databaseName: ROAD: Directory of Open Access Scholarly Resources customDbUrl: eissn: 2379-3708 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0001742058 issn: 2379-3708 databaseCode: M~E dateStart: 20160101 isFulltext: true titleUrlDefault: https://road.issn.org providerName: ISSN International Centre
link	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1Lb9NAEF6lhQMXVMQrUCojcSJy8WOzjyNEvASpeiginCw_xspWiRMlcemJP8CfZmb9iNMCAiQuduSsd6X5vmxmZufB2LOc-3mWZAmFVWiXywxcLXTmxtKHYQ7CC8AmCn-UJydqMtGnvd73JhfmYiaLQl1e6uV_hRqfIdiUOvsXcLeT4gP8jKDjFWHH6x8B_3ZmFgnqxJvFPHZxQjOzBWipxlKB94GhjBAYkMeejgzWMKfkqbE3mMfUzmtKQTwU-LUg7-yO7jp6T6HrZM23UTtxsirXBr6a1DqiP1Czgmx7iJOh7V9WPupXUJzHc9Ny8TOYKiuk2G6C6ykxxzpvv5TlynRdEr6NYq7SlztZALQ9dQJPR02iZ6d-SE08sNtdECJZQumpnb25Q8Hg5zu-pAqy56k5riVwrFCn8rtjEZ_l3KIdCC1DVfWIuVJm-3Q84lKRQbnHbgRyqCk-cPyt47CTPPCGqj4Zx2VfXFuUKkvXK-yqOddsl6shuB2d5uyA3a6NEedlRaI7rAfFXTb-FYGcikBORSDHEshpCOSMPWdLIKcl0D326c3rs9E7t2664aZcDDcuoP4ovDDHeyZEEuokSanJaBxTpcc8DvxMpyrXkAgVSOmnHKQAnYosBlAgwvtsv1gU8JA5IXCNGiVolXCufZ4AmgMS7Q2eZkNUnvrMa2QUpXVFemqMMousZSqDCCUc1RKOrIT77Hn7yrIqx_K7wU8bwUe4aZJY4gIW5ToioxoVU_x367MHFRDtdA2CfSZ3IGoHUEH23W8KM7WF2WsCPfrnNx-zW9vf0yHb36xKeMJuphcbs14dsT05UUeWlT8A0jK-ww
linkProvider	ISSN International Centre
openUrl	ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Glioblastoma-infiltrated+innate+immune+cells+resemble+M0+macrophage+phenotype&rft.jtitle=JCI+insight&rft.au=Gabrusiewicz%2C+Konrad&rft.au=Rodriguez%2C+Benjamin&rft.au=Wei%2C+Jun&rft.au=Hashimoto%2C+Yuuri&rft.date=2016-02-25&rft.pub=American+Society+for+Clinical+Investigation&rft.eissn=2379-3708&rft.volume=1&rft.issue=2&rft_id=info:doi/10.1172%2Fjci.insight.85841&rft_id=info%3Apmid%2F26973881&rft.externalDocID=PMC4784261
thumbnail_l	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2379-3708&client=summon
thumbnail_m	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2379-3708&client=summon
thumbnail_s	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2379-3708&client=summon