Differential effects of ceramide species on exocytosis in rat PC12 cells

Increases in several ceramide species have been shown by non-targeted lipid profiling (lipidomics) of the rat hippocampus after kainate lesions (Guan et al. FASEB J 20:1152-1161, 2006). This study was carried out to examine possible effects of ceramide species on exocytosis. Significant increase in...

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Vydáno v:Experimental brain research Ročník 183; číslo 2; s. 241 - 247
Hlavní autoři: Tang, Ning, Ong, Wei-Yi, Zhang, En-Ming, Chen, Peng, Yeo, Jin-Fei
Médium: Journal Article
Jazyk:angličtina
Vydáno: Berlin Springer 01.11.2007
Springer Nature B.V
Témata:
Anatomy
Animals
Arachnids
beta-Cyclodextrins - pharmacology
Biological and medical sciences
Cell culture
Cell Membrane - drug effects
Central nervous system
Ceramides - classification
Ceramides - pharmacology
Cholesterol
Drug Interactions
Electric Capacitance
Exocytosis - drug effects
Experiments
Fatty acids
Fundamental and applied biological sciences. Psychology
Green Fluorescent Proteins - metabolism
Hippocampus
Lesions
Lipids
Membrane Microdomains - drug effects
Microscopy
Microscopy, Fluorescence - methods
Morality
Motor control and motor pathways. Reflexes. Control centers of vegetative functions. Vestibular system and equilibration
Neurons
Neuropeptide Y - metabolism
Patch-Clamp Techniques
PC12 Cells - drug effects
Profiles
Rats
Time Factors
Vertebrates: nervous system and sense organs
United States > US
Singapore
Lipid raft
Membrane capacitance
Rat
Rodentia
Central nervous system
Glutamatergic neuron
Fluorescence
Sphingolipid
Glutamate
Exocytosis
Protein
Encephalon
Vertebrata
Mammalia
Synaptic transmission
Total internal reflection fluorescence microscopy
Excitotoxicity
Excitatory aminoacid
Neurotransmitter
Ceramide
Hippocampus
ISSN:0014-4819, 1432-1106
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Shrnutí:Increases in several ceramide species have been shown by non-targeted lipid profiling (lipidomics) of the rat hippocampus after kainate lesions (Guan et al. FASEB J 20:1152-1161, 2006). This study was carried out to examine possible effects of ceramide species on exocytosis. Significant increase in membrane capacitance in voltage-clamped rat pheochromocytoma (PC12) cells, an indication of exocytosis, was detected immediately after external application of C2, C6, and C18 ceramide. In contrast, no increase in capacitance was found after addition of C16 and C20 ceramide, or DMSO vehicle. The effect of ceramide on exocytosis was dependent on the integrity of lipid rafts. Treatment of cells with the cholesterol binding agent/disruptor of lipid rafts, methyl beta cyclodextrin, prior to addition of C18 ceramide suppressed the increase in capacitance induced by this lipid species. The ability of C2, C6 and C18 ceramide to trigger exocytosis was confirmed using total internal reflection fluorescence microscopy (TIRFM) experiments. External application of these species caused an exponential decrease in the number of subplasmalemmal neuropeptide Y (NPY)-enhanced green fluorescence protein (EGFP) labeled vesicles, indicating exocytosis. Interestingly, C18 is also the ceramide species that showed the greatest increase in the rat hippocampus after kainate excitotoxicity. It is postulated that C18 ceramide might facilitate exocytosis of glutamate from damaged neurons, thus propagating neuronal injury.
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ISSN:0014-4819
1432-1106
DOI:10.1007/s00221-007-1036-7