Glycosaminoglycans and syndecan-4 are involved in SDF-1/CXCL12-mediated invasion of human epitheloid carcinoma HeLa cells

In addition to their physiologic effects in inflammation and angiogenesis, chemokines are involved in cancer pathology. The CXC-chemokine stromal cell-derived factor-1 (SDF-1)/CXCL12 mediates its biological activities through activation of G protein-coupled receptor CXCR4 and binds to glycosaminogly...

Celý popis

Uloženo v:

Podrobná bibliografie
Vydáno v:	Biochimica et biophysica acta Ročník 1790; číslo 12; s. 1643 - 1650
Hlavní autoři:	Brule, Severine, Friand, Véronique, Sutton, Angela, Baleux, Françoise, Gattegno, Liliane, Charnaux, Nathalie
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	Netherlands Elsevier B.V 01.12.2009 Elsevier
Témata:	Anthracenes - pharmacology Biochemistry, Molecular Biology Carcinoma in Situ - metabolism Carcinoma in Situ - pathology Cell Movement - drug effects Chemokine Chemokine CXCL12 - antagonists & inhibitors Chemokine CXCL12 - pharmacology Chemokine CXCL12 - physiology Dose-Response Relationship, Drug Enzyme Inhibitors - pharmacology Female Glycosaminoglycan Glycosaminoglycans - physiology HeLa Cells Heterocyclic Compounds - pharmacology Humans Invasion Life Sciences Matrix Metalloproteinase 9 - metabolism Matrix Metalloproteinase 9 - physiology Neoplasm Invasiveness Proteoglycan SDF-1/CXCL12 Signal Transduction - drug effects Syndecan Syndecan-4 - physiology Uterine Cervical Neoplasms - metabolism Uterine Cervical Neoplasms - pathology Chemokine Proteoglycan Glycosaminoglycan SDF-1/CXCL12 Invasion Syndecan
ISSN:	0304-4165, 0006-3002, 0167-4889, 1872-8006
On-line přístup:	Získat plný text
Tagy:	Přidat tag Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!

Popis
Shrnutí:	In addition to their physiologic effects in inflammation and angiogenesis, chemokines are involved in cancer pathology. The CXC-chemokine stromal cell-derived factor-1 (SDF-1)/CXCL12 mediates its biological activities through activation of G protein-coupled receptor CXCR4 and binds to glycosaminoglycans (GAGs). Using Bio-coat cell migration chambers, specific antagonists, flow cytometry and RNA interference, we evaluate the involvement of heparan sulfate proteoglycans (HSPG) in the SDF-1/CXCL12-induced invasion of human cervix epitheloid carcinoma HeLa cells. The SDF-1/CXCL12-induced cell invasion is dependent on CXCR4. Furthermore, Protein Kinase C delta (PKC δ) and c-jun NH2-terminal kinase/stress-activated protein kinase (JNK/SAPK) are implicated in this event, but not extracellular signal-regulated kinase (ERK) 1/2. Moreover, the invasion of HeLa cells induced by SDF-1/CXCL12 was dependent on matrix metalloproteinase-9 (MMP-9). The pre-incubation of HeLa cells with heparin or with anti-heparan sulfate antibodies or with β- d-xyloside inhibited SDF-1/CXCL12-mediated cell invasion. Furthermore, the down-regulation of syndecan-4, a heparan sulfate proteoglycan, decreased SDF-1/CXCL12-mediated HeLa cell invasion. GAGs, probably on syndecan-4, are involved in SDF-1/CXCL12-mediated cell chemotaxis. These data suggest that targeting the glycosaminoglycan/chemokine interaction could be a new therapeutic approach for carcinomas in which SDF-1/CXCL12 is involved.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0304-4165 0006-3002 0167-4889 1872-8006
DOI:	10.1016/j.bbagen.2009.08.001