Phosphate Dosing in Drinking Water Distribution Systems Promotes Changes in Biofilm Structure and Functional Genetic Diversity

Water utilities treat drinking water by adding phosphate to prevent metal dissolution from water pipe work systems and particularly lead poisoning. Phosphate can be a limiting nutrient for microbial biofilms in DWDS, yet its effects on these microbial consortia are not well understood. This research...

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Veröffentlicht in:Frontiers in microbiology Jg. 11; S. 599091
Hauptverfasser: Rosales, Esther, Del Olmo, Gonzalo, Calero Preciado, Carolina, Douterelo, Isabel
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Switzerland Frontiers Media S.A 17.12.2020
Schlagworte:
biofilms
distribution networks
drinking water
Microbiology
phosphate
whole metagenomics
whole metagenomics
phosphate
drinking water
distribution networks
biofilms
ISSN:1664-302X, 1664-302X
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Zusammenfassung:Water utilities treat drinking water by adding phosphate to prevent metal dissolution from water pipe work systems and particularly lead poisoning. Phosphate can be a limiting nutrient for microbial biofilms in DWDS, yet its effects on these microbial consortia are not well understood. This research presents results from phosphate dosing experiments using a real scale chlorinated DWDS, comparing standard phosphate concentrations of United Kingdom drinking water (1 mgP/L) with a double dose (2 mgP/L) commonly used in plumbosolvency treatment. Biofilm development during phosphate treatment experiments was monitored using a holistic approach by combining metagenomics analysis, flow cytometry and SEM characterisation. The increase of phosphate levels in drinking water, reduced biofilm cell numbers and promoted the presence of poorly distributed biofilms on inner pipe surfaces. Metagenomics analysis using genetic markers (16S rRNA and ITS2) showed that phosphate influenced biofilm community structure, particularly fungal composition. Whole metagenome sequencing showed that phosphate enrichment favoured the presence of sequencing reads associated to ATPases, ion transporters and DNA-interacting proteins, whilst reads associated to nitrogen metabolism were predominant in control samples. This research brings new knowledge regarding the influence of phosphate treatment on the composition and structure of biofilms within DWDS, and the implications that this might have for the management of these systems.
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This article was submitted to Microbiotechnology, a section of the journal Frontiers in Microbiology
Edited by: Pascal E. Saikaly, King Abdullah University of Science and Technology, Saudi Arabia
Reviewed by: Dong Li, University of California, Santa Barbara, United States; Graciela Gonzalez-Gil, King Abdullah University of Science and Technology, Saudi Arabia
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2020.599091