Within-subject and between-subject biological variation estimates of 21 hematological parameters in 30 healthy subjects

The complete blood count (CBC) is used to evaluate health status in the contexts of various clinical situations such as anemia, infection, inflammation, trauma, malignancies, etc. To ensure safe clinical application of the CBC, reliable biological variation (BV) data are required. The study aim was...

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Vydané v:Clinical chemistry and laboratory medicine Ročník 56; číslo 8; s. 1309
Hlavní autori: Coşkun, Abdurrahman, Carobene, Anna, Kilercik, Meltem, Serteser, Mustafa, Sandberg, Sverre, Aarsand, Aasne K, Fernandez-Calle, Pilar, Jonker, Niels, Bartlett, William A, Díaz-Garzón, Jorge, Huet, Sibel, Kızıltaş, Cansu, Dalgakıran, Ilayda, Ugur, Esra, Unsal, Ibrahim
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: Germany 26.07.2018
Predmet:
hematology
preanalytical phase
biological variation
complete blood count
ISSN:1437-4331, 1437-4331
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Shrnutí:The complete blood count (CBC) is used to evaluate health status in the contexts of various clinical situations such as anemia, infection, inflammation, trauma, malignancies, etc. To ensure safe clinical application of the CBC, reliable biological variation (BV) data are required. The study aim was to define the BVs of CBC parameters employing a strict protocol. Blood samples, drawn from 30 healthy subjects (17 females, 13 males) once weekly for 10 weeks, were analyzed using a Sysmex XN 3000 instrument. The data were assessed for normality, trends, outliers and variance homogeneity prior to coefficient of variation (CV)-analysis of variance (ANOVA). Sex-stratified within-subject (CVI) and between-subjects (CVG) BV estimates were determined for 21 CBC parameters. For leukocyte parameters, with the exception of lymphocytes and basophils, significant differences were found between female/male CVI estimates. The mean values of all erythrocyte-, reticulocyte- and platelet parameters differed significantly between the sexes, except for mean corpuscular hemoglobin concentration, mean corpuscular volume and platelet numbers. Most CVI and CVG estimates appear to be lower than those previously published. Our study, based on a rigorous protocol, provides updated and more stringent BV estimates for CBC parameters. Sex stratification of data is necessary when exploring the significance of changes in consecutive results and when setting analytical performance specifications.
Bibliografia:ObjectType-Article-1
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ISSN:1437-4331
1437-4331
DOI:10.1515/cclm-2017-1155