RNA viruses, M satellites, chromosomal killer genes, and killer/nonkiller phenotypes in the 100-genomes S. cerevisiae strains

Abstract We characterized previously identified RNA viruses (L-A, L-BC, 20S, and 23S), L-A–dependent M satellites (M1, M2, M28, and Mlus), and M satellite–dependent killer phenotypes in the Saccharomyces cerevisiae 100-genomes genetic resource population. L-BC was present in all strains, albeit in 2...

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Published in:G3 : genes - genomes - genetics Vol. 13; no. 10
Main Authors: Vijayraghavan, Sriram, Kozmin, Stanislav G, Strope, Pooja K, Skelly, Daniel A, Magwene, Paul M, Dietrich, Fred S, McCusker, John H
Format: Journal Article
Language:English
Published: US Oxford University Press 30.09.2023
Subjects:
Genomes
Investigation
Phenotype
RNA Viruses - genetics
RNA, Double-Stranded
RNA, Viral - genetics
Saccharomyces cerevisiae - genetics
Satellites
Yeast
associations
phenotypes
killer
RNA virus
100-genomes strains
Saccharomyces cerevisiae
ISSN:2160-1836, 2160-1836
Online Access:Get full text
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Summary:Abstract We characterized previously identified RNA viruses (L-A, L-BC, 20S, and 23S), L-A–dependent M satellites (M1, M2, M28, and Mlus), and M satellite–dependent killer phenotypes in the Saccharomyces cerevisiae 100-genomes genetic resource population. L-BC was present in all strains, albeit in 2 distinct levels, L-BChi and L-BClo; the L-BC level is associated with the L-BC genotype. L-BChi, L-A, 20S, 23S, M1, M2, and Mlus (M28 was absent) were in fewer strains than the similarly inherited 2µ plasmid. Novel L-A–dependent phenotypes were identified. Ten M+ strains exhibited M satellite–dependent killing (K+) of at least 1 of the naturally M0 and cured M0 derivatives of the 100-genomes strains; in these M0 strains, sensitivities to K1+, K2+, and K28+ strains varied. Finally, to complement our M satellite–encoded killer toxin analysis, we assembled the chromosomal KHS1 and KHR1 killer genes and used naturally M0 and cured M0 derivatives of the 100-genomes strains to assess and characterize the chromosomal killer phenotypes.
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Conflicts of interest The authors declare no conflict of interest.
ISSN:2160-1836
2160-1836
DOI:10.1093/g3journal/jkad167