Cisplatin induces apoptosis in a human ovarian carcinoma cell line without concomitant internucleosomal degradation of DNA

After treatment of the human ovarian carcinoma cell line, CH1, with cisplatin, cells detached from the culture dish in a time- and dose-dependent fashion. These cells showed morphological changes indicative of apoptosis. Their DNA had not been degraded into oligonucleosomal fragments, but the DNA ha...

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Vydáno v:Experimental cell research Ročník 211; číslo 2; s. 231
Hlavní autoři: Ormerod, M G, O'Neill, C F, Robertson, D, Harrap, K R
Médium: Journal Article
Jazyk:angličtina
Vydáno: United States 01.04.1994
Témata:
Apoptosis - drug effects
Apoptosis - physiology
Cell Adhesion - drug effects
Cisplatin - pharmacology
DNA, Neoplasm - chemistry
DNA, Neoplasm - metabolism
Female
Humans
Microscopy, Electron
Molecular Weight
Nucleosomes - drug effects
Nucleosomes - metabolism
Ovarian Neoplasms - metabolism
Ovarian Neoplasms - pathology
Tumor Cells, Cultured - drug effects
Tumor Cells, Cultured - metabolism
Tumor Cells, Cultured - pathology
ISSN:0014-4827
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Shrnutí:After treatment of the human ovarian carcinoma cell line, CH1, with cisplatin, cells detached from the culture dish in a time- and dose-dependent fashion. These cells showed morphological changes indicative of apoptosis. Their DNA had not been degraded into oligonucleosomal fragments, but the DNA had been cut into larger fragments (30 kbp) of a size associated with chromatin loops. We conclude that cisplatin killed these ovarian cells by inducing apoptosis. However, in these cells, apoptosis was not accompanied by internucleosomal degradation of DNA. Our data are consistent with the hypothesis that the introduction of a double-strand break at a specific site in the chromatin loops is an early event in apoptosis. This degradation is accompanied by morphologically observable changes in chromatin structure. Internucleosomal degradation, when it occurs, is a late event.
Bibliografie:ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:0014-4827
DOI:10.1006/excr.1994.1082