Mother and Child T Cell Receptor Repertoires: Deep Profiling Study

The relationship between maternal and child immunity has been actively studied in the context of complications during pregnancy, autoimmune diseases, and haploidentical transplantation of hematopoietic stem cells and solid organs. Here, we have for the first time used high-throughput Illumina HiSeq...

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Vydáno v:Frontiers in immunology Ročník 4; s. 463
Hlavní autoři: Putintseva, Ekaterina V., Britanova, Olga V., Staroverov, Dmitriy B., Merzlyak, Ekaterina M., Turchaninova, Maria A., Shugay, Mikhail, Bolotin, Dmitriy A., Pogorelyy, Mikhail V., Mamedov, Ilgar Z., Bobrynina, Vlasta, Maschan, Mikhail, Lebedev, Yuri B., Chudakov, Dmitriy M.
Médium: Journal Article
Jazyk:angličtina
Vydáno: Switzerland Frontiers Media S.A 01.01.2013
Témata:
Autoimmune Diseases
haploidentical transplantation
Immunology
Maternal-Fetal Exchange
NGS
T cell receptor
TCR repertoires
autoimmune diseases
T cell receptor
public clonotypes
maternal-fetal exchange
haploidentical transplantation
TCR repertoires
microchimerism
NGS
ISSN:1664-3224, 1664-3224
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Shrnutí:The relationship between maternal and child immunity has been actively studied in the context of complications during pregnancy, autoimmune diseases, and haploidentical transplantation of hematopoietic stem cells and solid organs. Here, we have for the first time used high-throughput Illumina HiSeq sequencing to perform deep quantitative profiling of T cell receptor (TCR) repertoires for peripheral blood samples of three mothers and their six children. Advanced technology allowed accurate identification of 5 × 10(5) to 2 × 10(6) TCR beta clonotypes per individual. We performed comparative analysis of these TCR repertoires with the aim of revealing characteristic features that distinguish related mother-child pairs, such as relative TCR beta variable segment usage frequency and relative overlap of TCR beta complementarity-determining region 3 (CDR3) repertoires. We show that thymic selection essentially and similarly shapes the initial output of the TCR recombination machinery in both related and unrelated pairs, with minor effect from inherited differences. The achieved depth of TCR profiling also allowed us to test the hypothesis that mature T cells transferred across the placenta during pregnancy can expand and persist as functional microchimeric clones in their new host, using characteristic TCR beta CDR3 variants as clonal identifiers.
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This article was submitted to T Cell Biology, a section of the journal Frontiers in Immunology.
Edited by: Michal Or-Guil, Humboldt University Berlin, Germany
Reviewed by: Aridaman Pandit, Utrecht University, Netherlands; Nicole Wittenbrink, Humboldt University Berlin, Germany
ISSN:1664-3224
1664-3224
DOI:10.3389/fimmu.2013.00463