Genetic Basis and Clonal Population Structure of Antibiotic Resistance in Campylobacter jejuni Isolated From Broiler Carcasses in Belgium
Human campylobacteriosis is the leading food-borne zoonosis in industrialized countries. This study characterized the clonal population structure, antimicrobial resistance profiles and occurrence of antimicrobial resistance determinants of a set of strains isolated from broiler carcasses in Belgium....
Gespeichert in:
| Veröffentlicht in: | Frontiers in microbiology Jg. 9; S. 1014 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Journal Article |
| Sprache: | Englisch |
| Veröffentlicht: |
Switzerland
Frontiers Media S.A
17.05.2018
|
| Schlagworte: | |
| ISSN: | 1664-302X, 1664-302X |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| Abstract | Human campylobacteriosis is the leading food-borne zoonosis in industrialized countries. This study characterized the clonal population structure, antimicrobial resistance profiles and occurrence of antimicrobial resistance determinants of a set of
strains isolated from broiler carcasses in Belgium. Minimum inhibitory concentrations (MICs) against five commonly-used antibiotics (ciprofloxacin, nalidixic acid, tetracycline, gentamicin, and erythromycin) were determined for 204
isolates. More than half of the isolates were resistant to ciprofloxacin or nalidixic acid. In contrast, a lower percentage of screened isolates were resistant to gentamicin or erythromycin.
isolates resistant to ciprofloxacin and/or nalidixic acid were screened for the substitution T86I in the quinolone resistance determining region (QRDR) of the
gene, while
isolates resistant to tetracycline were screened for the presence of the
gene. These resistance determinants were observed in most but not all resistant isolates. Regarding resistance to erythromycin, different mutations occurred in diverse genetic loci, including mutations in the 23S rRNA gene, the
and
ribosomal genes, and the intergenic region between
and
. Interestingly, and contrary to previous reports, the A2075G transition mutation in the 23S rRNA gene was only found in one strain displaying a high level of resistance to erythromycin. Ultimately, molecular typing by multilocus sequence typing revealed that two sequence types (ST-824 and ST-2274) were associated to quinolones resistance by the presence of mutations in the gene
(
= 0.01). In addition, ST-2274 was linked to the CIP-NAL-TET-AMR multidrug resistant phenotype. In contrast, clonal complex CC-45 was linked to increased susceptibility to the tested antibiotics. The results obtained in this study provide better understanding of the phenotypic and the molecular basis of antibiotic resistance in
, unraveling some the mechanisms which confer antimicrobial resistance and particular clones associated to the carriage and spread of resistance genes. |
|---|---|
| AbstractList | Human campylobacteriosis is the leading food-borne zoonosis in industrialized countries. This study characterized the clonal population structure, antimicrobial resistance profiles and occurrence of antimicrobial resistance determinants of a set of
strains isolated from broiler carcasses in Belgium. Minimum inhibitory concentrations (MICs) against five commonly-used antibiotics (ciprofloxacin, nalidixic acid, tetracycline, gentamicin, and erythromycin) were determined for 204
isolates. More than half of the isolates were resistant to ciprofloxacin or nalidixic acid. In contrast, a lower percentage of screened isolates were resistant to gentamicin or erythromycin.
isolates resistant to ciprofloxacin and/or nalidixic acid were screened for the substitution T86I in the quinolone resistance determining region (QRDR) of the
gene, while
isolates resistant to tetracycline were screened for the presence of the
gene. These resistance determinants were observed in most but not all resistant isolates. Regarding resistance to erythromycin, different mutations occurred in diverse genetic loci, including mutations in the 23S rRNA gene, the
and
ribosomal genes, and the intergenic region between
and
. Interestingly, and contrary to previous reports, the A2075G transition mutation in the 23S rRNA gene was only found in one strain displaying a high level of resistance to erythromycin. Ultimately, molecular typing by multilocus sequence typing revealed that two sequence types (ST-824 and ST-2274) were associated to quinolones resistance by the presence of mutations in the gene
(
= 0.01). In addition, ST-2274 was linked to the CIP-NAL-TET-AMR multidrug resistant phenotype. In contrast, clonal complex CC-45 was linked to increased susceptibility to the tested antibiotics. The results obtained in this study provide better understanding of the phenotypic and the molecular basis of antibiotic resistance in
, unraveling some the mechanisms which confer antimicrobial resistance and particular clones associated to the carriage and spread of resistance genes. Human campylobacteriosis is the leading food-borne zoonosis in industrialized countries. This study characterized the clonal population structure, antimicrobial resistance profiles and occurrence of antimicrobial resistance determinants of a set of Campylobacter jejuni strains isolated from broiler carcasses in Belgium. Minimum inhibitory concentrations (MICs) against five commonly-used antibiotics (ciprofloxacin, nalidixic acid, tetracycline, gentamicin, and erythromycin) were determined for 204 C. jejuni isolates. More than half of the isolates were resistant to ciprofloxacin or nalidixic acid. In contrast, a lower percentage of screened isolates were resistant to gentamicin or erythromycin. C. jejuni isolates resistant to ciprofloxacin and/or nalidixic acid were screened for the substitution T86I in the quinolone resistance determining region (QRDR) of the gyrA gene, while C. jejuni isolates resistant to tetracycline were screened for the presence of the tet(O) gene. These resistance determinants were observed in most but not all resistant isolates. Regarding resistance to erythromycin, different mutations occurred in diverse genetic loci, including mutations in the 23S rRNA gene, the rplD and rplV ribosomal genes, and the intergenic region between cmeR and cmeABC. Interestingly, and contrary to previous reports, the A2075G transition mutation in the 23S rRNA gene was only found in one strain displaying a high level of resistance to erythromycin. Ultimately, molecular typing by multilocus sequence typing revealed that two sequence types (ST-824 and ST-2274) were associated to quinolones resistance by the presence of mutations in the gene gyrA (p = 0.01). In addition, ST-2274 was linked to the CIP-NAL-TET-AMR multidrug resistant phenotype. In contrast, clonal complex CC-45 was linked to increased susceptibility to the tested antibiotics. The results obtained in this study provide better understanding of the phenotypic and the molecular basis of antibiotic resistance in C. jejuni, unraveling some the mechanisms which confer antimicrobial resistance and particular clones associated to the carriage and spread of resistance genes. Human campylobacteriosis is the leading food-borne zoonosis in industrialized countries. This study characterized the clonal population structure, antimicrobial resistance profiles and occurrence of antimicrobial resistance determinants of a set of Campylobacter jejuni strains isolated from broiler carcasses in Belgium. Minimum inhibitory concentrations (MICs) against five commonly-used antibiotics (ciprofloxacin, nalidixic acid, tetracycline, gentamicin, and erythromycin) were determined for 204 C. jejuni isolates. More than half of the isolates were resistant to ciprofloxacin or nalidixic acid. In contrast, a lower percentage of screened isolates were resistant to gentamicin or erythromycin. C. jejuni isolates resistant to ciprofloxacin and/or nalidixic acid were screened for the substitution T86I in the quinolone resistance determining region (QRDR) of the gyrA gene, while C. jejuni isolates resistant to tetracycline were screened for the presence of the tet(O) gene. These resistance determinants were observed in most but not all resistant isolates. Regarding resistance to erythromycin, different mutations occurred in diverse genetic loci, including mutations in the 23S rRNA gene, the rplD and rplV ribosomal genes, and the intergenic region between cmeR and cmeABC. Interestingly, and contrary to previous reports, the A2075G transition mutation in the 23S rRNA gene was only found in one strain displaying a high level of resistance to erythromycin. Ultimately, molecular typing by multilocus sequence typing revealed that two sequence types (ST-824 and ST-2274) were associated to quinolones resistance by the presence of mutations in the gene gyrA (p = 0.01). In addition, ST-2274 was linked to the CIP-NAL-TET-AMR multidrug resistant phenotype. In contrast, clonal complex CC-45 was linked to increased susceptibility to the tested antibiotics. The results obtained in this study provide better understanding of the phenotypic and the molecular basis of antibiotic resistance in C. jejuni, unraveling some the mechanisms which confer antimicrobial resistance and particular clones associated to the carriage and spread of resistance genes.Human campylobacteriosis is the leading food-borne zoonosis in industrialized countries. This study characterized the clonal population structure, antimicrobial resistance profiles and occurrence of antimicrobial resistance determinants of a set of Campylobacter jejuni strains isolated from broiler carcasses in Belgium. Minimum inhibitory concentrations (MICs) against five commonly-used antibiotics (ciprofloxacin, nalidixic acid, tetracycline, gentamicin, and erythromycin) were determined for 204 C. jejuni isolates. More than half of the isolates were resistant to ciprofloxacin or nalidixic acid. In contrast, a lower percentage of screened isolates were resistant to gentamicin or erythromycin. C. jejuni isolates resistant to ciprofloxacin and/or nalidixic acid were screened for the substitution T86I in the quinolone resistance determining region (QRDR) of the gyrA gene, while C. jejuni isolates resistant to tetracycline were screened for the presence of the tet(O) gene. These resistance determinants were observed in most but not all resistant isolates. Regarding resistance to erythromycin, different mutations occurred in diverse genetic loci, including mutations in the 23S rRNA gene, the rplD and rplV ribosomal genes, and the intergenic region between cmeR and cmeABC. Interestingly, and contrary to previous reports, the A2075G transition mutation in the 23S rRNA gene was only found in one strain displaying a high level of resistance to erythromycin. Ultimately, molecular typing by multilocus sequence typing revealed that two sequence types (ST-824 and ST-2274) were associated to quinolones resistance by the presence of mutations in the gene gyrA (p = 0.01). In addition, ST-2274 was linked to the CIP-NAL-TET-AMR multidrug resistant phenotype. In contrast, clonal complex CC-45 was linked to increased susceptibility to the tested antibiotics. The results obtained in this study provide better understanding of the phenotypic and the molecular basis of antibiotic resistance in C. jejuni, unraveling some the mechanisms which confer antimicrobial resistance and particular clones associated to the carriage and spread of resistance genes. |
| Author | Arguello, Hector Elhadidy, Mohamed Dierick, Katelijne Duarte, Alexandra Botteldoorn, Nadine Álvarez-Ordóñez, Avelino Miller, William G. |
| AuthorAffiliation | 2 University of Science and Technology, Zewail City of Science and Technology , Giza , Egypt 4 Genomics and Animal Breeding, Department of Genetics, Faculty of Veterinary Science, University of Córdoba , Córdoba , Spain 5 Department of Food Hygiene and Technology, Institute of Food Science and Technology, University of León , León , Spain 3 Produce Safety and Microbiology Research Unit, Agricultural Research Service, U.S. Department of Agriculture , Albany, CA , United States 7 National Reference Laboratory for Campylobacter, Scientific Institute of Public Health (WIV-ISP), Scientific Service: Foodborne Pathogens , Brussels , Belgium 6 Laboratory of Food Microbiology and Food Preservation, Department of Food Safety and Food Quality, Faculty of Bioscience Engineering, Ghent University , Gent , Belgium 1 Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Mansoura University , Mansoura , Egypt |
| AuthorAffiliation_xml | – name: 5 Department of Food Hygiene and Technology, Institute of Food Science and Technology, University of León , León , Spain – name: 2 University of Science and Technology, Zewail City of Science and Technology , Giza , Egypt – name: 6 Laboratory of Food Microbiology and Food Preservation, Department of Food Safety and Food Quality, Faculty of Bioscience Engineering, Ghent University , Gent , Belgium – name: 3 Produce Safety and Microbiology Research Unit, Agricultural Research Service, U.S. Department of Agriculture , Albany, CA , United States – name: 7 National Reference Laboratory for Campylobacter, Scientific Institute of Public Health (WIV-ISP), Scientific Service: Foodborne Pathogens , Brussels , Belgium – name: 1 Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Mansoura University , Mansoura , Egypt – name: 4 Genomics and Animal Breeding, Department of Genetics, Faculty of Veterinary Science, University of Córdoba , Córdoba , Spain |
| Author_xml | – sequence: 1 givenname: Mohamed surname: Elhadidy fullname: Elhadidy, Mohamed – sequence: 2 givenname: William G. surname: Miller fullname: Miller, William G. – sequence: 3 givenname: Hector surname: Arguello fullname: Arguello, Hector – sequence: 4 givenname: Avelino surname: Álvarez-Ordóñez fullname: Álvarez-Ordóñez, Avelino – sequence: 5 givenname: Alexandra surname: Duarte fullname: Duarte, Alexandra – sequence: 6 givenname: Katelijne surname: Dierick fullname: Dierick, Katelijne – sequence: 7 givenname: Nadine surname: Botteldoorn fullname: Botteldoorn, Nadine |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/29867900$$D View this record in MEDLINE/PubMed |
| BookMark | eNp1kk1vGyEQhldVqiZNc--p4tiLXdgPFi6V4lWTWorUqh9Sb4iFwcViwQU2Un5C_3WxnUZJpXIANDPvM6B5X1YnPnioqtcEL5uG8Xdmsmpc1piwJSaYtM-qM0Jpu2hw_ePk0f20ukhpi8tqcV32F9VpzRntOcZn1e9r8JCtQiuZbELSazS44KVDn8NudjLb4NHXHGeV5wgoGHTpsx1t2Gu-QNFk6RUg69Egp92dC6NUGSLawnb2Fq1TKBDQ6CqGCa1isK4kBxmVTAnSXrcCt7Hz9Kp6bqRLcHF_nlffrz58Gz4ubj5dr4fLm4VqaZ0XjKtOYdCa1SPvR9ZyqUalTa1oj3tuSDcyQ3iJMUMx1FLzhhhZvs5NTzhpzqv1kauD3IpdtJOMdyJIKw6BEDdCxvI7BwJI14M2qiWMtqynXPY973hBS62N1oX1_sjazeMEWoHPUbon0KcZb3-KTbgVHae0Y7gA3t4DYvg1Q8piskmBc9JDmJOocYdb1rWcl9I3j3s9NPk7y1KAjwUqhpQimIcSgsXeMeLgGLF3jDg4pkjoPxJl82Hm5bXW_V_4Bx4Sygk |
| CitedBy_id | crossref_primary_10_1099_mgen_0_000834 crossref_primary_10_1186_s13104_020_05150_x crossref_primary_10_1016_j_onehlt_2025_100990 crossref_primary_10_1080_03079457_2023_2260322 crossref_primary_10_3390_foods11223554 crossref_primary_10_1371_journal_pone_0223804 crossref_primary_10_1111_apm_13005 crossref_primary_10_3390_antibiotics9060308 crossref_primary_10_1016_j_cimid_2019_06_001 crossref_primary_10_3389_fmicb_2023_1271551 crossref_primary_10_3390_microorganisms10051067 crossref_primary_10_1002_fsn3_3152 crossref_primary_10_3390_antibiotics13090904 crossref_primary_10_1016_j_psj_2024_103485 crossref_primary_10_1128_AAC_01622_20 crossref_primary_10_3390_microorganisms10010046 crossref_primary_10_3389_fmicb_2021_622275 crossref_primary_10_1371_journal_pntd_0012916 crossref_primary_10_1371_journal_pone_0227535 crossref_primary_10_3390_antibiotics11050536 crossref_primary_10_3390_ijms26104657 crossref_primary_10_1016_j_fm_2023_104349 crossref_primary_10_3389_fmicb_2020_554784 crossref_primary_10_1016_j_ijfoodmicro_2022_109912 crossref_primary_10_14202_vetworld_2020_1430_1438 crossref_primary_10_1080_10408398_2021_1883548 crossref_primary_10_1111_tbed_13046 crossref_primary_10_1371_journal_pone_0318516 crossref_primary_10_3390_antibiotics11070830 crossref_primary_10_1016_j_vetmic_2020_108652 crossref_primary_10_1080_00071668_2020_1758300 crossref_primary_10_1080_10408398_2023_2246554 crossref_primary_10_1186_s13099_022_00517_w crossref_primary_10_3390_antibiotics9110790 crossref_primary_10_3390_genes12081131 crossref_primary_10_3390_vetsci7030121 crossref_primary_10_1016_j_ijfoodmicro_2024_110940 crossref_primary_10_3390_antibiotics12050890 |
| Cites_doi | 10.1128/AAC.00450-08 10.1128/JCM.01086-12 10.4161/viru.23753 10.1016/j.ijantimicag.2005.08.019 10.1016/j.foodcont.2005.10.011 10.1016/j.mimet.2005.03.013 10.3201/eid0701.010104 10.1016/j.foodcont.2012.06.044 10.1128/AAC.05516-11 10.1016/j.femsle.2005.09.019 10.1093/jac/48.2.235 10.1016/j.mcp.2004.02.001 10.1128/AAC.03039-14 10.1093/jac/dkl210 10.1128/JCM.43.5.2315-2329.2005 10.3389/fmicb.2017.02240 10.1017/S0950268813003245 10.1111/mec.14176 10.1016/j.ijfoodmicro.2007.01.006 10.1128/CMR.00006-15 10.2217/17460913.4.2.189 10.1128/AAC.49.7.2753-2759.2005 10.1089/mdr.2005.11.40 10.1128/AAC.48.9.3442-3450.2004 10.1080/03079457.2012.734915 10.1128/AEM.00168-09 10.1016/j.ijfoodmicro.2018.04.004 10.1128/JCM.37.10.3276-3280.1999 10.2903/j.efsa.2015.4329 10.1016/S0924-8579(01)00425-3 10.1371/journal.pone.0081796 10.1016/j.ijid.2015.11.005 10.1128/AEM.72.5.3600-3607.2006 10.1128/JB.187.21.7417-7424.2005 10.1128/JCM.00355-14 10.1093/jac/dkm300 10.1089/mdr.2013.0110 10.1089/fpd.2011.1042 10.3389/fmicb.2016.00072 10.1051/vetres:2001127 10.3389/fmicb.2015.01169 10.1128/AAC.45.1.1-12.2001 10.1086/599374 10.1128/AAC.49.1.457-460.2005 10.1128/AAC.49.3.1067-1075.2005 10.1093/jac/dkq268 10.1093/ps/80.9.1390 |
| ContentType | Journal Article |
| Copyright | Copyright © 2018 Elhadidy, Miller, Arguello, Álvarez-Ordóñez, Duarte, Dierick and Botteldoorn. 2018 Elhadidy, Miller, Arguello, Álvarez-Ordóñez, Duarte, Dierick and Botteldoorn |
| Copyright_xml | – notice: Copyright © 2018 Elhadidy, Miller, Arguello, Álvarez-Ordóñez, Duarte, Dierick and Botteldoorn. 2018 Elhadidy, Miller, Arguello, Álvarez-Ordóñez, Duarte, Dierick and Botteldoorn |
| DBID | AAYXX CITATION NPM 7X8 5PM DOA |
| DOI | 10.3389/fmicb.2018.01014 |
| DatabaseName | CrossRef PubMed MEDLINE - Academic PubMed Central (Full Participant titles) DOAJ Directory of Open Access Journals |
| DatabaseTitle | CrossRef PubMed MEDLINE - Academic |
| DatabaseTitleList | PubMed MEDLINE - Academic |
| Database_xml | – sequence: 1 dbid: DOA name: DOAJ (Directory of Open Access Journals) url: https://www.doaj.org/ sourceTypes: Open Website – sequence: 2 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 3 dbid: 7X8 name: MEDLINE - Academic url: https://search.proquest.com/medline sourceTypes: Aggregation Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Biology |
| EISSN | 1664-302X |
| ExternalDocumentID | oai_doaj_org_article_e157edfc418648769a77959bc8addfdd PMC5966580 29867900 10_3389_fmicb_2018_01014 |
| Genre | Journal Article |
| GroupedDBID | 53G 5VS 9T4 AAFWJ AAKDD AAYXX ACGFO ACGFS ADBBV ADRAZ AENEX AFPKN ALMA_UNASSIGNED_HOLDINGS AOIJS BAWUL BCNDV CITATION DIK ECGQY GROUPED_DOAJ GX1 HYE KQ8 M48 M~E O5R O5S OK1 PGMZT RNS RPM ACXDI IPNFZ NPM RIG 7X8 5PM |
| ID | FETCH-LOGICAL-c462t-89c5c0edd82b97b849acbcdf2c67079f15b8f19cbc8f60e2ad931fa4029f71913 |
| IEDL.DBID | DOA |
| ISICitedReferencesCount | 45 |
| ISICitedReferencesURI | http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000432384100002&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| ISSN | 1664-302X |
| IngestDate | Fri Oct 03 12:46:09 EDT 2025 Tue Sep 30 16:49:21 EDT 2025 Sun Nov 09 12:15:40 EST 2025 Thu Apr 03 06:59:45 EDT 2025 Tue Nov 18 21:25:53 EST 2025 Sat Nov 29 02:07:19 EST 2025 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Keywords | resistance genes mutations MLST antibiotic resistance broiler |
| Language | English |
| License | This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c462t-89c5c0edd82b97b849acbcdf2c67079f15b8f19cbc8f60e2ad931fa4029f71913 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Xu Jia, Chengde Medical College, China; Ben Pascoe, University of Bath, United Kingdom This article was submitted to Food Microbiology, a section of the journal Frontiers in Microbiology Edited by: Paula Teixeira, Universidade Católica Portuguesa, Portugal |
| OpenAccessLink | https://doaj.org/article/e157edfc418648769a77959bc8addfdd |
| PMID | 29867900 |
| PQID | 2050485499 |
| PQPubID | 23479 |
| ParticipantIDs | doaj_primary_oai_doaj_org_article_e157edfc418648769a77959bc8addfdd pubmedcentral_primary_oai_pubmedcentral_nih_gov_5966580 proquest_miscellaneous_2050485499 pubmed_primary_29867900 crossref_primary_10_3389_fmicb_2018_01014 crossref_citationtrail_10_3389_fmicb_2018_01014 |
| PublicationCentury | 2000 |
| PublicationDate | 2018-05-17 |
| PublicationDateYYYYMMDD | 2018-05-17 |
| PublicationDate_xml | – month: 05 year: 2018 text: 2018-05-17 day: 17 |
| PublicationDecade | 2010 |
| PublicationPlace | Switzerland |
| PublicationPlace_xml | – name: Switzerland |
| PublicationTitle | Frontiers in microbiology |
| PublicationTitleAlternate | Front Microbiol |
| PublicationYear | 2018 |
| Publisher | Frontiers Media S.A |
| Publisher_xml | – name: Frontiers Media S.A |
| References | Elhadidy (B12) 2018; 275 Cody (B6) 2012; 50 Rodrigo (B43) 2007; 18 Kaakoush (B28) 2015; 28 Pascoe (B41) 2017; 26 Kittl (B29) 2013; 14 Collignon (B7) 2009; 49 Wu (B49) 2014; 52 Zirnstein (B51) 1999; 37 Iovine (B25) 2013; 4 (B48) 2011 Caldwell (B5) 2008; 52 Florez-Cuadrado (B16) 2017; 8 Kovač (B31) 2014; 42 Humphrey (B24) 2007; 117 Miller (B39) 2005; 43 Dionisi (B10) 2004; 18 Luangtonkum (B37) 2012; 56 Klein-Jöbstl (B30) 2016; 7 Corcoran (B8) 2005; 253 Haanperä (B22) 2005; 49 Luangtongkum (B35) 2009; 4 Lin (B33); 49 EFSA (B11) 2015; 13 (B26) 2006 Kovač (B32) 2015; 6 Giacomelli (B17) 2012; 41 Luangtongkum (B36) 2006; 72 Lin (B34); 187 Niwa (B40) 2001; 18 Giacomelli (B18) 2014; 20 Gibreel (B21) 2004; 48 Mattheus (B38) 2012; 9 Belanger (B3) 2007; 60 Habib (B23) 2009; 75 Van Looveren (B45) 2001; 48 Vacher (B44) 2005; 11 Pérez-Boto (B42) 2010; 65 B13 B15 Wang (B47) 2014; 58 Engberg (B14) 2001; 7 Corcoran (B9) 2006; 27 Alonso (B2) 2005; 63 Gibreel (B19) 2006; 58 Jeffrey (B27) 2001; 80 Vester (B46) 2001; 45 Gibreel (B20) 2005; 4 Bolton (B4) 2013; 30 Aarestrup (B1) 2001; 32 Zhou (B50) 2016; 42 |
| References_xml | – volume: 52 start-page: 3947 year: 2008 ident: B5 article-title: Development, stability, and molecular mechanisms of macrolide resistance in Campylobacter jejuni publication-title: Antimicrob. Agents Chemother. doi: 10.1128/AAC.00450-08 – volume: 50 start-page: 3193 year: 2012 ident: B6 article-title: A longitudinal 6-year study of the molecular epidemiology of clinical Campylobacter isolates in Oxfordshire, United Kingdom publication-title: J. Clin. Microbiol. doi: 10.1128/JCM.01086-12 – volume: 4 start-page: 230 year: 2013 ident: B25 article-title: Resistance mechanisms in Campylobacter jejuni publication-title: Virulence doi: 10.4161/viru.23753 – volume: 27 start-page: 40 year: 2006 ident: B9 article-title: An investigation of the molecular mechanisms contributing to high-level erythromycin resistance in Campylobacter publication-title: Int. J. Antimicrob. Agents doi: 10.1016/j.ijantimicag.2005.08.019 – volume: 18 start-page: 321 year: 2007 ident: B43 article-title: Antimicrobial resistance of Campylobacter spp. isolated from broilers in small poultry processing operations in Trinidad publication-title: Food Control doi: 10.1016/j.foodcont.2005.10.011 – volume: 63 start-page: 99 year: 2005 ident: B2 article-title: MAMA-PCR assay for the detection of point mutations associated with high-level erythromycin resistance in Campylobacter jejuni and Campylobacter coli strains publication-title: J. Microbiol. Methods. doi: 10.1016/j.mimet.2005.03.013 – volume: 7 start-page: 24 year: 2001 ident: B14 article-title: Quinolone and macrolide resistance in Campylobacter jejuni and C. coli: resistance mechanisms and trends in human isolates publication-title: Emerg. Infect. Dis. doi: 10.3201/eid0701.010104 – volume: 30 start-page: 222 year: 2013 ident: B4 article-title: A study of the molecular basis of quinolone and macrolide resistance in a selection of Campylobacter isolates from intensive poultry flocks publication-title: Food Control doi: 10.1016/j.foodcont.2012.06.044 – volume: 56 start-page: 1300 year: 2012 ident: B37 article-title: Impaired fitness and transmission of macrolide-resistant Campylobacter jejuni in its natural host publication-title: Antimicrob. Agents Chemother. doi: 10.1128/AAC.05516-11 – volume: 253 start-page: 39 year: 2005 ident: B8 article-title: Relative contribution of target gene mutation and efflux to varying quinolone resistance in Irish Campylobacter isolates publication-title: FEMS Microbiol. Lett. doi: 10.1016/j.femsle.2005.09.019 – volume: 48 start-page: 235 year: 2001 ident: B45 article-title: Antimicrobial susceptibilities of Campylobacter strains isolated from food animals in Belgium publication-title: J. Antimicrob. Chemother. doi: 10.1093/jac/48.2.235 – ident: B13 – volume: 18 start-page: 255 year: 2004 ident: B10 article-title: Identification of ciprofloxacin-resistant Campylobacter jejuni and analysis of the gyrA gene by the Light Cycler mutation assay publication-title: Mol. Cell Probes doi: 10.1016/j.mcp.2004.02.001 – volume: 58 start-page: 5405 year: 2014 ident: B47 article-title: Emergence of multidrug-resistant Campylobacter species isolates with a horizontally acquired rRNA methylase publication-title: Antimicrob. Agents Chemother. doi: 10.1128/AAC.03039-14 – volume: 58 start-page: 243 year: 2006 ident: B19 article-title: Macrolide resistance in Campylobacter jejuni and Campylobacter coli publication-title: J. Antimicrob. Chemother. doi: 10.1093/jac/dkl210 – volume: 43 start-page: 2315 year: 2005 ident: B39 article-title: Extended multilocus sequence typing system for Campylobacter coli, C. lari, C. upsaliensis, and C. helveticus. J. Clin. Microbiol doi: 10.1128/JCM.43.5.2315-2329.2005 – volume: 8 start-page: 2240 year: 2017 ident: B16 article-title: Genome comparison of erythromycin resistant Campylobacter from turkeys identifies hosts and pathways for horizontal spread of erm(B) genes publication-title: Front. Microbiol. doi: 10.3389/fmicb.2017.02240 – volume: 42 start-page: 2595 year: 2014 ident: B31 article-title: The evidence for clonal spreading of quinolone resistance with a particular clonal complex of Campylobacter jejuni publication-title: Epidemiol. Infect. doi: 10.1017/S0950268813003245 – volume: 26 start-page: 4497 year: 2017 ident: B41 article-title: Local genes for local bacteria: evidence of allopatry in the genomes of transatlantic Campylobacter populations publication-title: Mol. Ecol doi: 10.1111/mec.14176 – volume: 117 start-page: 237 year: 2007 ident: B24 article-title: Campylobacters as zoonotic pathogens: a food production perspective publication-title: Int. J. Food Microbiol. doi: 10.1016/j.ijfoodmicro.2007.01.006 – volume: 28 start-page: 687 year: 2015 ident: B28 article-title: Global epidemiology of Campylobacter infection publication-title: Clin. Microbiol. Rev. doi: 10.1128/CMR.00006-15 – volume: 4 start-page: 189 year: 2009 ident: B35 article-title: Antibiotic resistance in Campylobacter: emergence, transmission and persistence publication-title: Future Microbiol. doi: 10.2217/17460913.4.2.189 – volume: 4 start-page: 2753 year: 2005 ident: B20 article-title: Macrolide resistance in Campylobacter jejuni and Campylobacter coli: molecular mechanism and stability of the resistance phenotype publication-title: Antimicrob. Agents Chemother. doi: 10.1128/AAC.49.7.2753-2759.2005 – volume: 11 start-page: 40 year: 2005 ident: B44 article-title: Detection of mutations associated with macrolide resistance in thermophilic Campylobacter spp. by real-time PCR publication-title: Microbial. Drug Resist. doi: 10.1089/mdr.2005.11.40 – volume: 48 start-page: 3442 year: 2004 ident: B21 article-title: Incidence of antibiotic resistance in Campylobacter jejuni isolated in Alberta, Canada, from 1999 to 2002, with special reference to tet (O)-mediated tetracycline resistance publication-title: J. Antimicrob. Chemother. doi: 10.1128/AAC.48.9.3442-3450.2004 – volume: 41 start-page: 579 year: 2012 ident: B17 article-title: Molecular characterization and genotypic antimicrobial resistance analysis of Campylobacter jejuni and Campylobacter coli isolated from broiler flocks in northern Italy publication-title: Avian Pathol doi: 10.1080/03079457.2012.734915 – volume-title: Critically Important Antimicrobials for Human Medicine, 3rd ev Edn year: 2011 ident: B48 – volume: 75 start-page: 4264 year: 2009 ident: B23 article-title: Clonal population structure and antimicrobial resistance of Campylobacter jejuni in chicken meat from Belgium publication-title: Appl. Environ. Microbiol. doi: 10.1128/AEM.00168-09 – volume: 275 start-page: 66 year: 2018 ident: B12 article-title: Orthogonal typing methods identify genetic diversity among Belgian Campylobacter jejuni strains isolated over a decade from poultry and cases of sporadic human illness publication-title: Int. J. Food Microbiol doi: 10.1016/j.ijfoodmicro.2018.04.004 – volume: 37 start-page: 3276 year: 1999 ident: B51 article-title: Ciprofloxacin resistance in Campylobacter jejuni isolates: detection of gyrA resistance mutations by mismatch amplification mutation assay PCR and DNA sequence analysis publication-title: J. Clin. Microbiol. doi: 10.1128/JCM.37.10.3276-3280.1999 – volume: 13 start-page: 4329 year: 2015 ident: B11 article-title: The european union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2014 publication-title: EFSA J. doi: 10.2903/j.efsa.2015.4329 – volume: 18 start-page: 359 year: 2001 ident: B40 article-title: Rapid detection of mutations associated with resistance to erythromycin in Campylobacter jejuni/coli by PCR and line probe assay publication-title: Int. J. Antimicrob. Agents doi: 10.1016/S0924-8579(01)00425-3 – volume: 14 start-page: e81796 year: 2013 ident: B29 article-title: Source attribution of human Campylobacter isolates by MLST and fla-typing and association of genotypes with quinolone resistance publication-title: PLoS ONE doi: 10.1371/journal.pone.0081796 – volume: 42 start-page: 28 year: 2016 ident: B50 article-title: A seventeen-year observation of the antimicrobial susceptibility of clinical Campylobacter jejuni and the molecular mechanisms of erythromycin-resistant isolates in Beijing, China publication-title: Int. J. Infect. Dis. doi: 10.1016/j.ijid.2015.11.005 – volume: 72 start-page: 3600 year: 2006 ident: B36 article-title: Effect of conventional and organic production practices on the prevalence and antimicrobial resistance of Campylobacter spp. in poultry publication-title: Appl. Environ. Microbiol. doi: 10.1128/AEM.72.5.3600-3607.2006 – volume: 187 start-page: 7417 ident: B34 article-title: Bile salts modulate expression of the CmeABC multidrug efflux pump in Campylobacter jejuni publication-title: J. Bacteriol. doi: 10.1128/JB.187.21.7417-7424.2005 – volume: 52 start-page: 1853 year: 2014 ident: B49 article-title: Genetic diversity and antimicrobial susceptibility of Campylobacter jejuni isolates associated with sheep abortion in the United States and Great Britain publication-title: J. Clin. Microbiol. doi: 10.1128/JCM.00355-14 – volume: 60 start-page: 715 year: 2007 ident: B3 article-title: Macrolide-resistant Campylobacter: the meat of the matter publication-title: J. Antimicrob. Chemother. doi: 10.1093/jac/dkm300 – volume: 20 start-page: 181 year: 2014 ident: B18 article-title: Antimicrobial resistance of Campylobacter jejuni and Campylobacter coli from poultry in Italy publication-title: Microb. Drug Resist. doi: 10.1089/mdr.2013.0110 – volume: 9 start-page: 465 year: 2012 ident: B38 article-title: Trend analysis of antimicrobial resistance in Campylobacter jejuni and Campylobacter coli isolated from Belgian pork and poultry meat products using surveillance data of 2004–2009 publication-title: Foodborne Pathog. Dis. doi: 10.1089/fpd.2011.1042 – volume: 7 start-page: 72 year: 2016 ident: B30 article-title: Multilocus sequence typing and antimicrobial resistance of Campylobacter jejuni isolated from dairy calves in Austria publication-title: Front. Microbiol. doi: 10.3389/fmicb.2016.00072 – volume-title: ISO 10272 Microbiology of Food and Animal Feeding Stuff - Horizontal Method for Detection and Enumeration of Campylobacter spp. - Part 1: Enrichment Method; part 2: Enumeration Method year: 2006 ident: B26 – volume: 32 start-page: 311 year: 2001 ident: B1 article-title: Antimicrobial resistance of thermophilic Campylobacter publication-title: Vet. Res. doi: 10.1051/vetres:2001127 – volume: 6 start-page: 1169 year: 2015 ident: B32 article-title: High genetic similarity of ciprofloxacin-resistant Campylobacter jejuni in central Europe publication-title: Front. Microbiol. doi: 10.3389/fmicb.2015.01169 – volume: 45 start-page: 1 year: 2001 ident: B46 article-title: Macrolide resistance conferred by base substitutions in 23S rRNA publication-title: Antimicrob. Agents Chemother. doi: 10.1128/AAC.45.1.1-12.2001 – volume: 49 start-page: 132 year: 2009 ident: B7 article-title: World Health Organization ranking of antimicrobials according to their importance in human medicine: a critical step for developing risk management strategies for the use of antimicrobials in food production animals publication-title: Clin. Infect. Dis. doi: 10.1086/599374 – volume: 49 start-page: 457 year: 2005 ident: B22 article-title: Detection and quantification of macrolide resistance mutations at positions 2058 and 2059 of the 23S rRNA gene by pyrosequencing publication-title: Antimicrob. Agents Chemother. doi: 10.1128/AAC.49.1.457-460.2005 – ident: B15 – volume: 49 start-page: 1067 ident: B33 article-title: CmeR functions as a transcriptional repressor for the multidrug efflux pump CmeABC in Campylobacter jejuni publication-title: Antimicrob. Agents Chemother. doi: 10.1128/AAC.49.3.1067-1075.2005 – volume: 65 start-page: 2083 year: 2010 ident: B42 article-title: Study of the molecular mechanisms involved in high-level macrolide resistance of Spanish Campylobacter jejuni and Campylobacter coli strains publication-title: J. Antimicrob. Chemother. doi: 10.1093/jac/dkq268 – volume: 80 start-page: 1390 year: 2001 ident: B27 article-title: Prevalence of campylobacter spp. from skin, crop, and intestine of commercial broiler chicken carcasses at processing publication-title: Poul. Sci. doi: 10.1093/ps/80.9.1390 |
| SSID | ssj0000402000 |
| Score | 2.3804624 |
| Snippet | Human campylobacteriosis is the leading food-borne zoonosis in industrialized countries. This study characterized the clonal population structure,... |
| SourceID | doaj pubmedcentral proquest pubmed crossref |
| SourceType | Open Website Open Access Repository Aggregation Database Index Database Enrichment Source |
| StartPage | 1014 |
| SubjectTerms | antibiotic resistance broiler Microbiology MLST mutations resistance genes |
| Title | Genetic Basis and Clonal Population Structure of Antibiotic Resistance in Campylobacter jejuni Isolated From Broiler Carcasses in Belgium |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/29867900 https://www.proquest.com/docview/2050485499 https://pubmed.ncbi.nlm.nih.gov/PMC5966580 https://doaj.org/article/e157edfc418648769a77959bc8addfdd |
| Volume | 9 |
| WOSCitedRecordID | wos000432384100002&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| journalDatabaseRights | – providerCode: PRVAON databaseName: DOAJ (Directory of Open Access Journals) customDbUrl: eissn: 1664-302X dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000402000 issn: 1664-302X databaseCode: DOA dateStart: 20100101 isFulltext: true titleUrlDefault: https://www.doaj.org/ providerName: Directory of Open Access Journals – providerCode: PRVHPJ databaseName: ROAD: Directory of Open Access Scholarly Resources customDbUrl: eissn: 1664-302X dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000402000 issn: 1664-302X databaseCode: M~E dateStart: 20100101 isFulltext: true titleUrlDefault: https://road.issn.org providerName: ISSN International Centre |
| link | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1Lb9QwELagAokL4t3wqIzEhUNonDixfexWXcGBquIh7S3yE1JtHZR0kXrhzr9mxtmudhGCC5cc_JCtmfE8NJNvCHlVgQlxyttc27LJeeAyN9xiEYARtamrwrGQmk2I01O5WKizrVZfWBM2wQNPhDv0rBbeBcuZbMC5bpQW2B7bWAkvMziH2he8nq1gKulgDIuKYspLQhSmgE2dNVjKJd-k_rQ7dijB9f_Jx_y9VHLL9szvkbtrp5EeTZe9T274-IDcntpIXj0kPxE7GqboTI_dSHV09HiJHjY927Tnoh8TUOxq8LQP9CjiryI97vngR3Qhgfe0ixRTEVeIEYIYzvTcn69iR9-BfIJL6uh86C8oBO6gSQZYOliNKWPcN_PLL93q4hH5PD_5dPw2X7dYyC1vystcKlvbwjsnS6OEkVxpa6wLpW0QOi-w2sjAFIzJ0BS-1E5VLGigrgoCQr3qMdmLffT7hKrasMo71TjuufNMNwGMH2feiICeRkYOrwne2jX-OLbBWLYQhyCL2sSiFlnUJhZl5PVmx7cJe-Mva2fIw806RM1OAyBL7VqW2n_JUkZeXktAC68MUyc6-n41wkE1qDqMpTPyZJKIzVGlQtDCosiI2JGVnbvszsTua0LyriHYrGXx9H9c_hm5g-TAygYmnpM9kCr_gtyy3y-7cTggN8VCHqRHAt_3P05-ASEYHBo |
| linkProvider | Directory of Open Access Journals |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Genetic+Basis+and+Clonal+Population+Structure+of+Antibiotic+Resistance+in+Campylobacter+jejuni+Isolated+From+Broiler+Carcasses+in+Belgium&rft.jtitle=Frontiers+in+microbiology&rft.au=Elhadidy%2C+Mohamed&rft.au=Miller%2C+William+G&rft.au=Arguello%2C+Hector&rft.au=%C3%81lvarez-Ord%C3%B3%C3%B1ez%2C+Avelino&rft.date=2018-05-17&rft.issn=1664-302X&rft.eissn=1664-302X&rft.volume=9&rft.spage=1014&rft_id=info:doi/10.3389%2Ffmicb.2018.01014&rft_id=info%3Apmid%2F29867900&rft.externalDocID=29867900 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1664-302X&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1664-302X&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1664-302X&client=summon |