Quantitation and Comparison of Phenotypic Heterogeneity Among Single Cells of Monoclonal Microbial Populations

Phenotypic heterogeneity within microbial populations arises even when the cells are exposed to putatively constant and homogeneous conditions. The outcome of this phenomenon can affect the whole function of the population, resulting in, for example, new "adapted" metabolic strategies and...

Celý popis

Uloženo v:
Podrobná bibliografie
Vydáno v:Frontiers in microbiology Ročník 10; s. 2814
Hlavní autoři: Calabrese, Federica, Voloshynovska, Iryna, Musat, Florin, Thullner, Martin, Schlömann, Michael, Richnow, Hans H., Lambrecht, Johannes, Müller, Susann, Wick, Lukas Y., Musat, Niculina, Stryhanyuk, Hryhoriy
Médium: Journal Article
Jazyk:angličtina
Vydáno: Switzerland Frontiers Media S.A 20.12.2019
Témata:
anabolic activity
flow cytometry
Microbiology
multimodality
phenotypic heterogeneity
single-cell resolution
SIP–nanoSIMS
single-cell resolution
SIP–nanoSIMS
Zipf's law
heterogeneity quantitation
phenotypic heterogeneity
flow cytometry
multimodality
anabolic activity
ISSN:1664-302X, 1664-302X
On-line přístup:Získat plný text
Tagy: Přidat tag
Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!
Popis
Shrnutí:Phenotypic heterogeneity within microbial populations arises even when the cells are exposed to putatively constant and homogeneous conditions. The outcome of this phenomenon can affect the whole function of the population, resulting in, for example, new "adapted" metabolic strategies and impacting its fitness at given environmental conditions. Accounting for phenotypic heterogeneity becomes thus necessary, due to its relevance in medical and applied microbiology as well as in environmental processes. Still, a comprehensive evaluation of this phenomenon requires a common and unique method of quantitation, which allows for the comparison between different studies carried out with different approaches. Consequently, in this study, two widely applicable indices for quantitation of heterogeneity were developed. The heterogeneity coefficient (HC) is valid when the population follows unimodal activity, while the differentiation tendency index (DTI) accounts for heterogeneity implying outbreak of subpopulations and multimodal activity. We demonstrated the applicability of HC and DTI for heterogeneity quantitation on stable isotope probing with nanoscale secondary ion mass spectrometry (SIP-nanoSIMS), flow cytometry, and optical microscopy datasets. The HC was found to provide a more accurate and precise measure of heterogeneity, being at the same time consistent with the coefficient of variation (CV) applied so far. The DTI is able to describe the differentiation in single-cell activity within monoclonal populations resolving subpopulations with low cell abundance, individual cells with similar phenotypic features (e.g., isotopic content close to natural abundance, as detected with nanoSIMS). The developed quantitation approach allows for a better understanding on the impact and the implications of phenotypic heterogeneity in environmental, medical and applied microbiology, microbial ecology, cell biology, and biotechnology.
Bibliografie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Edited by: Frank Schreiber, Federal Institute for Materials Research and Testing (BAM), Germany
Reviewed by: Michael Konopka, University of Akron, United States; Frank Delvigne, University of Liège, Belgium; Andrij Rovenchak, Ivan Franko National University of Lviv, Ukraine
This article was submitted to Microbial Physiology and Metabolism, a section of the journal Frontiers in Microbiology
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2019.02814