Bacteriophage Procurement for Therapeutic Purposes

Bacteriophages (phages), discovered 100 years ago, are able to infect and destroy only bacterial cells. In the current crisis of antibiotic efficacy, phage therapy is considered as a supplementary or even alternative therapeutic approach. Evolution of multidrug-resistant and pandrug-resistant bacter...

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Bibliographic Details
Published in:Frontiers in microbiology Vol. 7; p. 1177
Main Authors: Weber-Dąbrowska, Beata, Jończyk-Matysiak, Ewa, Żaczek, Maciej, Łobocka, Małgorzata, Łusiak-Szelachowska, Marzanna, Górski, Andrzej
Format: Journal Article
Language:English
Published: Switzerland Frontiers Media S.A 12.08.2016
Subjects:
antibiotic resistance
bacteriophage isolation
experimental phage therapy
Microbiology
therapeutic phages
treatment of bacterial infections
experimental phage therapy
antibiotic resistance
bacteriophage isolation
therapeutic phages
treatment of bacterial infections
ISSN:1664-302X, 1664-302X
Online Access:Get full text
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Summary:Bacteriophages (phages), discovered 100 years ago, are able to infect and destroy only bacterial cells. In the current crisis of antibiotic efficacy, phage therapy is considered as a supplementary or even alternative therapeutic approach. Evolution of multidrug-resistant and pandrug-resistant bacterial strains poses a real threat, so it is extremely important to have the possibility to isolate new phages for therapeutic purposes. Our phage laboratory and therapy center has extensive experience with phage isolation, characterization, and therapeutic application. In this article we present current progress in bacteriophages isolation and use for therapeutic purposes, our experience in this field and its practical implications for phage therapy. We attempt to summarize the state of the art: properties of phages, the methods for their isolation, criteria of phage selection for therapeutic purposes and limitations of their use. Perspectives for the use of genetically engineered phages to specifically target bacterial virulence-associated genes are also briefly presented.
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Reviewed by: Lei Dai, Iowa State University, USA; Andrei A. Zimin, Russian Academy of Sciences, Russia; Jean-Paul Pirnay, Queen Astrid Military Hospital, Belgium
Edited by: Peter Mullany, University College London, UK
This article was submitted to Antimicrobials, Resistance and Chemotherapy, a section of the journal Frontiers in Microbiology
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2016.01177