Compositional and Quantitative Insights Into Bacterial and Archaeal Communities of South Pacific Deep-Sea Sponges (Demospongiae and Hexactinellida)

In the present study, we profiled bacterial and archaeal communities from 13 phylogenetically diverse deep-sea sponge species (Demospongiae and Hexactinellida) from the South Pacific by 16S rRNA-gene amplicon sequencing. Additionally, the associated bacteria and archaea were quantified by real-time...

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Vydáno v:Frontiers in microbiology Ročník 11; s. 716
Hlavní autoři: Steinert, Georg, Busch, Kathrin, Bayer, Kristina, Kodami, Sahar, Arbizu, Pedro Martinez, Kelly, Michelle, Mills, Sadie, Erpenbeck, Dirk, Dohrmann, Martin, Wörheide, Gert, Hentschel, Ute, Schupp, Peter J.
Médium: Journal Article
Jazyk:angličtina
Vydáno: Switzerland Frontiers Media S.A 24.04.2020
Témata:
16S rRNA amplicons
archaea
bacteria
Demospongiae
Hexactinellida
Microbiology
Porifera
Demospongiae
quantitative real-time PCR (qPCR)
Hexactinellida
bacteria
Porifera
South Pacific Ocean
16S rRNA amplicons
archaea
ISSN:1664-302X, 1664-302X
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Shrnutí:In the present study, we profiled bacterial and archaeal communities from 13 phylogenetically diverse deep-sea sponge species (Demospongiae and Hexactinellida) from the South Pacific by 16S rRNA-gene amplicon sequencing. Additionally, the associated bacteria and archaea were quantified by real-time qPCR. Our results show that bacterial communities from the deep-sea sponges are mostly host-species specific similar to what has been observed for shallow-water demosponges. The archaeal deep-sea sponge community structures are different from the bacterial community structures in that they are almost completely dominated by a single family, which are the ammonia-oxidizing genera within the Nitrosopumilaceae. Remarkably, the archaeal communities are mostly specific to individual sponges (rather than sponge-species), and this observation applies to both hexactinellids and demosponges. Finally, archaeal 16s gene numbers, as detected by quantitative real-time PCR, were up to three orders of magnitude higher than in shallow-water sponges, highlighting the importance of the archaea for deep-sea sponges in general.
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Edited by: Zhiyong Li, Shanghai Jiao Tong University, China
This article was submitted to Microbial Symbioses, a section of the journal Frontiers in Microbiology
Reviewed by: Nicole De Voogd, Naturalis Biodiversity Center, Netherlands; Marco J. L. Coolen, Curtin University, Australia; Lu Fan, Southern University of Science and Technology, China
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2020.00716