Isolation, identification, and partial cDNA cloning of genomic RNA of jaagsiekte retrovirus, the etiological agent of sheep pulmonary adenomatosis

The genome of the jaagsiekte (JS) retrovirus (JSRV), the etiological agent of sheep pulmonary adenomatosis (jaagsiekte), has been identified, isolated, and partly cloned. The JSRV genome is ca. 8.7 kb long. cDNA of the genomic RNA was synthesized and cloned. A clone, JS 46.1, was isolated and charac...

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Veröffentlicht in:Journal of virology Jg. 65; H. 9; S. 5061
Hauptverfasser: York, D F, Vigne, R, Verwoerd, D W, Querat, G
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States 01.09.1991
Schlagworte:
Amino Acid Sequence
Animals
Blotting, Northern
Centrifugation, Isopycnic
Cloning, Molecular
DNA - genetics
Gene Products, env - genetics
Lentivirus - isolation & purification
Lung - microbiology
Molecular Sequence Data
Pulmonary Adenomatosis, Ovine - microbiology
Retroviridae - genetics
Retroviridae - isolation & purification
RNA, Viral - genetics
RNA, Viral - isolation & purification
Sheep
ISSN:0022-538X
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Zusammenfassung:The genome of the jaagsiekte (JS) retrovirus (JSRV), the etiological agent of sheep pulmonary adenomatosis (jaagsiekte), has been identified, isolated, and partly cloned. The JSRV genome is ca. 8.7 kb long. cDNA of the genomic RNA was synthesized and cloned. A clone, JS 46.1, was isolated and characterized. It has an insert of 2.1 kb which hybridizes to the same 8.7-kb RNA in all the JSRV-infected sheep lung washes tested but does not hybridize to maedi-visna virus, a sheep lentivirus often found coinfecting JSRV-infected lungs. Comparison of the amino acid sequence encoded by JS 46.1 with those encoded by other retroviruses revealed that JSRV has homology to the type D and B oncoviruses and to human endogenous retrovirus.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0022-538X
DOI:10.1128/jvi.65.9.5061-5067.1991