Rapid and cost-effective epitope mapping using PURE ribosome display coupled with next-generation sequencing and bioinformatics

A novel, efficient and cost-effective approach for epitope identification of an antibody has been developed using a ribosome display platform. This platform, known as PURE ribosome display, utilizes an Escherichia coli-based reconstituted cell-free protein synthesis system (PURE system). It stabiliz...

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Bibliographic Details
Published in:	Journal of bioscience and bioengineering Vol. 137; no. 4; pp. 321 - 328
Main Authors:	Jia, Beixi, Ojima-Kato, Teruyo, Kojima, Takaaki, Nakano, Hideo
Format:	Journal Article
Language:	English
Published:	Japan Elsevier B.V 01.04.2024
Subjects:	algorithms Anti-HA tag antibody bioinformatics Cell-free protein synthesis cost effectiveness Epitope mapping epitopes hemagglutinins monoclonal antibodies oligonucleotides peptide libraries Python program Ribosome display ribosomes Epitope mapping Anti-HA tag antibody Python program Cell-free protein synthesis Ribosome display
ISSN:	1389-1723, 1347-4421, 1347-4421
Online Access:	Get full text
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