Life of RISC: Formation, action, and degradation of RNA-induced silencing complex

Small RNAs regulate a wide variety of biological processes by repressing the expression of target genes at the transcriptional and post-transcriptional levels. To achieve these functions, small RNAs form RNA-induced silencing complex (RISC) together with a member of the Argonaute (AGO) protein famil...

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Bibliographic Details
Published in:Molecular cell Vol. 82; no. 1; p. 30
Main Authors: Iwakawa, Hiro-Oki, Tomari, Yukihide
Format: Journal Article
Language:English
Published: United States 06.01.2022
Subjects:
Animals
Argonaute Proteins - genetics
Argonaute Proteins - metabolism
Humans
MicroRNAs - genetics
MicroRNAs - metabolism
Protein Binding
Protein Stability
Proteolysis
RNA - genetics
RNA - metabolism
RNA Stability
RNA, Messenger - genetics
RNA, Messenger - metabolism
RNA, Small Interfering - genetics
RNA, Small Interfering - metabolism
RNA-Induced Silencing Complex - genetics
RNA-Induced Silencing Complex - metabolism
ISSN:1097-4164, 1097-4164
Online Access:Get more information
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Summary:Small RNAs regulate a wide variety of biological processes by repressing the expression of target genes at the transcriptional and post-transcriptional levels. To achieve these functions, small RNAs form RNA-induced silencing complex (RISC) together with a member of the Argonaute (AGO) protein family. RISC is directed by its bound small RNA to target complementary RNAs and represses their expression through mRNA cleavage, degradation, and/or translational repression. Many different factors fine-tune RISC activity and stability-from guide-target RNA complementarity to the recruitment of other protein partners to post-translational modifications of RISC itself. Here, we review recent progress in understanding RISC formation, action, and degradation, and discuss new, intriguing questions in the field.
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ISSN:1097-4164
1097-4164
DOI:10.1016/j.molcel.2021.11.026