Propofol postconditioning protects H9c2 cells from hypoxia/reoxygenation injury by inducing autophagy via the SAPK/JNK pathway

Propofol postconditioning (P-PostC) offers cardioprotection in mice, and the upregulation of autophagy protects cardiac cells against ischemia/reperfusion injury. The present study aimed to examine the effects of P-PostC on the induction of autophagy and its potential roles in hypoxia/reoxygenation...

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Vydáno v:	Molecular medicine reports Ročník 17; číslo 3; s. 4573 - 4580
Hlavní autoři:	Li, Hao, Zhang, Xuan, Tan, Jian, Sun, Li, Xu, Long-He, Jiang, Yu-Ge, Lou, Jing-Sheng, Shi, Xue-Yin, Mi, Wei-Dong
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	Greece D.A. Spandidos 01.03.2018 Spandidos Publications Spandidos Publications UK Ltd
Témata:	Adenine - analogs & derivatives Adenine - pharmacology Animals Anthracenes - pharmacology Apoptosis Autophagy Autophagy - drug effects Biotechnology c-Jun protein Cardiomyocytes cardioprotection Cell culture Cell Hypoxia Cell survival Dosage and administration Drug interactions Health aspects Heart Heart diseases Hypoxia Immunofluorescence Ischemia ischemic postconditioning JNK Mitogen-Activated Protein Kinases - antagonists & inhibitors JNK Mitogen-Activated Protein Kinases - metabolism JNK protein Kinases L-Lactate dehydrogenase L-Lactate Dehydrogenase - metabolism Lactic acid MAP kinase MAP Kinase Signaling System - drug effects Microtubule-Associated Proteins - metabolism myocardial ischemia Myocytes Myocytes, Cardiac - cytology Myocytes, Cardiac - metabolism Observations Oxygen - pharmacology Phagocytosis Phosphorylation Phosphorylation - drug effects Propofol Propofol - pharmacology Protein kinases Proteins Rats Reperfusion Sequestosome-1 Protein - metabolism Stem cells Studies Transcription factors Transmission electron microscopy Western blotting United States > US
ISSN:	1791-2997, 1791-3004, 1791-3004
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Shrnutí:	Propofol postconditioning (P-PostC) offers cardioprotection in mice, and the upregulation of autophagy protects cardiac cells against ischemia/reperfusion injury. The present study aimed to examine the effects of P-PostC on the induction of autophagy and its potential roles in hypoxia/reoxygenation (H/R) injury. Rat heart-derived H9c2 cells were exposed to H/R, comprising 6 h of hypoxia followed by 4 h of reoxygenation, as well as postconditioning with various concentrations of propofol at the onset of reperfusion. Lactate dehydrogenase (LDH) activity and the rate of cell apoptosis were measured to evaluate the degree of cardiomyocyte H/R injury. The induction of autophagy in myocytes subjected to H/R injury and P-PostC was detected by western blotting and immunofluorescence. Furthermore, the activation of c-Jun N-terminal kinase (JNK) in cells treated with P-PostC with or without co-treatment with SP600125, an inhibitor of JNK, was also determined by western blotting. P-PostC reduced the activity of LDH in the culture medium and the percentage of apoptotic cells compared with cells in the untreated H/R group. In addition, P-PostC induced autophagy and promoted survival signaling in H9c2 cardiac myoblast cells. The inhibition of autophagy by 3-methyladenine treatment diminished the cardioprotective effects of P-PostC. These results indicated that propofol postconditioning promoted cell survival through the induction of autophagy in H9c2 cardiac cells, and that the stress-activated protein kinase/JNK survival pathway may be partly involved in P-PostC-induced autophagy.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23
ISSN:	1791-2997 1791-3004 1791-3004
DOI:	10.3892/mmr.2018.8424