Investigating the relationship between the cell cycle and apoptosis using flow cytometry

Methods for using flow cytometry to investigate the relationship between the induction of apoptosis and the cell cycle are discussed. Methods for following cell cycle progression are also briefly reviewed. The methods are illustrated using a specific example of the effect of withdrawing an essential...

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Bibliographic Details
Published in:Journal of immunological methods Vol. 265; no. 1; pp. 73 - 80
Main Author: Ormerod, Michael G
Format: Journal Article
Language:English
Published: Netherlands Elsevier B.V 01.07.2002
Subjects:
Animals
Apoptosis
Bromodeoxyuridine - metabolism
Cell Cycle
Flow Cytometry
Humans
In Situ Nick-End Labeling
PBS
Flow cytometry
IL
Tdt
BrdUrd
DAPI
PARP
SBIP
FITC
TUNEL
PE
Cell cycle
PI
ddUTP
Apoptosis
ISSN:0022-1759, 1872-7905
Online Access:Get full text
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Summary:Methods for using flow cytometry to investigate the relationship between the induction of apoptosis and the cell cycle are discussed. Methods for following cell cycle progression are also briefly reviewed. The methods are illustrated using a specific example of the effect of withdrawing an essential growth factor from a cell line.
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ISSN:0022-1759
1872-7905
DOI:10.1016/S0022-1759(02)00071-6