A toolbox facilitating stable transfection of Eimeria species

Stable transfection of Eimeria species has been difficult to achieve because of the obligate requirement for in vivo amplification and selection of the parasites. Strategies to generate and stabilise populations of transfected Eimeria tenella are described here, together with the identification of o...

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Veröffentlicht in:	Molecular and biochemical parasitology Jg. 162; H. 1; S. 77 - 86
Hauptverfasser:	Clark, Julie D., Billington, Karen, Bumstead, Janene M., Oakes, Richard D., Soon, Puay Eng, Sopp, Paul, Tomley, Fiona M., Blake, Damer P.
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	Netherlands Elsevier B.V 01.11.2008 Amsterdam: Elsevier
Schlagworte:	Animals Antiprotozoal Agents - pharmacology Apicomplexa Bacterial Proteins - genetics Chicken Chickens Coccidiosis - parasitology Coccidiosis - veterinary Drug Resistance - genetics Eimeria Eimeria tenella Eimeria tenella - genetics Eimeria tenella - growth & development Electroporation fluorescence Fluorescent reporter protein Genes, Reporter - genetics Luminescent Proteins - genetics mutants oocysts parasites plasmids Plasmids - genetics Poultry Diseases - parasitology pyrimethamine Pyrimethamine - pharmacology reporter genes restriction endonucleases Specific Pathogen-Free Organisms Sporozoites Toxoplasma gondii Transfection Transfection - methods Transformation, Genetic Wisconsin Wisconsin YFP Apicomplexa YFPm Transfection MDBK Eimeria Fluorescent reporter protein DHFR-TS REMI Chicken FACS
ISSN:	0166-6851, 1872-9428
Online-Zugang:	Volltext
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