Treatment with 815-nm diode laser induces long-lasting expression of 72-kDa heat shock protein in normal rat skin

Background We previously reported that skin closure is improved by photoirradiation of the wound margins with an 815‐nm diode laser system. Objectives To determine whether the beneficial effects of laser treatment involve the overexpression of the inducible 72‐kDa heat shock protein, Hsp70. Methods ...

Full description

Saved in:
Bibliographic Details
Published in:British journal of dermatology (1951) Vol. 144; no. 2; pp. 260 - 266
Main Authors: Souil, E., Capon, A., Mordon, S., Dinh-Xuan, A.T., Polla, B.S., Bachelet, M.
Format: Journal Article
Language:English
Published: Oxford, UK Blackwell Science Ltd 01.02.2001
Blackwell
Oxford University Press
Subjects:
Animals
Biological and medical sciences
Dermatology
Epidermis - metabolism
Epidermis - radiation effects
heat shock proteins
Heat-Shock Proteins - metabolism
HSP70 Heat-Shock Proteins - metabolism
HSP72 Heat-Shock Proteins
immunocytochemistry
Investigative techniques, diagnostic techniques (general aspects)
laser
Laser Therapy
Male
Medical sciences
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
rat skin
Rats
Skin - metabolism
Skin - radiation effects
wound healing
Wound Healing - physiology
Wound Healing - radiation effects
Immunohistochemistry
Vertebrata
Mammalia
Rat
Animal
Rodentia
Laser
Heat shock protein
Skin
Biological activity
ISSN:0007-0963, 1365-2133
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Background We previously reported that skin closure is improved by photoirradiation of the wound margins with an 815‐nm diode laser system. Objectives To determine whether the beneficial effects of laser treatment involve the overexpression of the inducible 72‐kDa heat shock protein, Hsp70. Methods Expression of Hsp70 was investigated by immunocytochemistry in normal hairless rat dorsal skin and compared with its expression after laser photoirradiation. Results Constitutive expression of Hsp70 was mainly confined to the upper epidermal layer. Laser irradiation further increased epidermal expression of Hsp70 while inducing de novo synthesis of the protein in dermal structures, particularly around blood vessels, hair follicles and sebaceous glands. Laser‐induced expression of Hsp70 was still present 7 days after photoirradiation. Conclusions Laser‐induced expression of Hsp70 might contribute to improved tissue regeneration and wound healing.
Bibliography:istex:DBC34C72157D860C7780DBFA3460257EB57D3192
ark:/67375/WNG-918QRB94-K
ArticleID:BJD4010
ObjectType-Article-1
SourceType-Scholarly Journals-1
content type line 14
ObjectType-Feature-2
content type line 23
ISSN:0007-0963
1365-2133
DOI:10.1046/j.1365-2133.2001.04010.x