Ultrasensitive DNA chip: gene expression profile analysis without RNA amplification

We have developed a new DNA chip whose substrate has a unique minute columnar array structure made of plastic. The DNA chip exhibits ultrahigh sensitivity, up to 100-fold higher than that of reference DNA chips, which makes it possible to monitor gene expression profiles even with very small amounts...

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Vydáno v:	Journal of biochemistry (Tokyo) Ročník 139; číslo 4; s. 697
Hlavní autoři:	Nagino, Kunihisa, Nomura, Osamu, Takii, Yuki, Myomoto, Akira, Ichikawa, Makiko, Nakamura, Fumio, Higasa, Masashi, Akiyama, Hideo, Nobumasa, Hitoshi, Shiojima, Satoshi, Tsujimoto, Gozoh
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	England 01.04.2006
Témata:	Gene Expression Profiling - instrumentation Gene Expression Profiling - methods Microscopy, Electron, Scanning Nucleic Acid Amplification Techniques - methods Nucleic Acid Hybridization - methods Oligonucleotide Array Sequence Analysis - instrumentation Oligonucleotide Array Sequence Analysis - methods Plasminogen Activators - genetics Reproducibility of Results Reverse Transcriptase Polymerase Chain Reaction - methods RNA - genetics RNA - metabolism RNA, Messenger - genetics RNA, Messenger - metabolism Statistics, Nonparametric
ISSN:	0021-924X
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Popis
Shrnutí:	We have developed a new DNA chip whose substrate has a unique minute columnar array structure made of plastic. The DNA chip exhibits ultrahigh sensitivity, up to 100-fold higher than that of reference DNA chips, which makes it possible to monitor gene expression profiles even with very small amounts of RNA (0.1-0.01 microg of total RNA) without amplification. Differential expression ratios obtained with the new DNA chip were validated against those obtained with quantitative real-time PCR assays. This novel microarray technology would be a powerful tool for monitoring gene expression profiles, especially for clinical diagnosis.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0021-924X
DOI:	10.1093/jb/mvj086