Ultrasensitive DNA chip: gene expression profile analysis without RNA amplification
We have developed a new DNA chip whose substrate has a unique minute columnar array structure made of plastic. The DNA chip exhibits ultrahigh sensitivity, up to 100-fold higher than that of reference DNA chips, which makes it possible to monitor gene expression profiles even with very small amounts...
Gespeichert in:
| Veröffentlicht in: | Journal of biochemistry (Tokyo) Jg. 139; H. 4; S. 697 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , |
| Format: | Journal Article |
| Sprache: | Englisch |
| Veröffentlicht: |
England
01.04.2006
|
| Schlagworte: | |
| ISSN: | 0021-924X |
| Online-Zugang: | Weitere Angaben |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| Abstract | We have developed a new DNA chip whose substrate has a unique minute columnar array structure made of plastic. The DNA chip exhibits ultrahigh sensitivity, up to 100-fold higher than that of reference DNA chips, which makes it possible to monitor gene expression profiles even with very small amounts of RNA (0.1-0.01 microg of total RNA) without amplification. Differential expression ratios obtained with the new DNA chip were validated against those obtained with quantitative real-time PCR assays. This novel microarray technology would be a powerful tool for monitoring gene expression profiles, especially for clinical diagnosis. |
|---|---|
| AbstractList | We have developed a new DNA chip whose substrate has a unique minute columnar array structure made of plastic. The DNA chip exhibits ultrahigh sensitivity, up to 100-fold higher than that of reference DNA chips, which makes it possible to monitor gene expression profiles even with very small amounts of RNA (0.1-0.01 microg of total RNA) without amplification. Differential expression ratios obtained with the new DNA chip were validated against those obtained with quantitative real-time PCR assays. This novel microarray technology would be a powerful tool for monitoring gene expression profiles, especially for clinical diagnosis.We have developed a new DNA chip whose substrate has a unique minute columnar array structure made of plastic. The DNA chip exhibits ultrahigh sensitivity, up to 100-fold higher than that of reference DNA chips, which makes it possible to monitor gene expression profiles even with very small amounts of RNA (0.1-0.01 microg of total RNA) without amplification. Differential expression ratios obtained with the new DNA chip were validated against those obtained with quantitative real-time PCR assays. This novel microarray technology would be a powerful tool for monitoring gene expression profiles, especially for clinical diagnosis. We have developed a new DNA chip whose substrate has a unique minute columnar array structure made of plastic. The DNA chip exhibits ultrahigh sensitivity, up to 100-fold higher than that of reference DNA chips, which makes it possible to monitor gene expression profiles even with very small amounts of RNA (0.1-0.01 microg of total RNA) without amplification. Differential expression ratios obtained with the new DNA chip were validated against those obtained with quantitative real-time PCR assays. This novel microarray technology would be a powerful tool for monitoring gene expression profiles, especially for clinical diagnosis. |
| Author | Myomoto, Akira Higasa, Masashi Nakamura, Fumio Nomura, Osamu Shiojima, Satoshi Nagino, Kunihisa Takii, Yuki Ichikawa, Makiko Akiyama, Hideo Tsujimoto, Gozoh Nobumasa, Hitoshi |
| Author_xml | – sequence: 1 givenname: Kunihisa surname: Nagino fullname: Nagino, Kunihisa email: Kunihisa_nagino@nts.toray.co.jp organization: New Frontiers Research Laboratories, Toray Industries, Inc., Kanagawa 248-8555. Kunihisa_nagino@nts.toray.co.jp – sequence: 2 givenname: Osamu surname: Nomura fullname: Nomura, Osamu – sequence: 3 givenname: Yuki surname: Takii fullname: Takii, Yuki – sequence: 4 givenname: Akira surname: Myomoto fullname: Myomoto, Akira – sequence: 5 givenname: Makiko surname: Ichikawa fullname: Ichikawa, Makiko – sequence: 6 givenname: Fumio surname: Nakamura fullname: Nakamura, Fumio – sequence: 7 givenname: Masashi surname: Higasa fullname: Higasa, Masashi – sequence: 8 givenname: Hideo surname: Akiyama fullname: Akiyama, Hideo – sequence: 9 givenname: Hitoshi surname: Nobumasa fullname: Nobumasa, Hitoshi – sequence: 10 givenname: Satoshi surname: Shiojima fullname: Shiojima, Satoshi – sequence: 11 givenname: Gozoh surname: Tsujimoto fullname: Tsujimoto, Gozoh |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/16672270$$D View this record in MEDLINE/PubMed |
| BookMark | eNo1kMlOwzAYhH0oogsceAHkE7dQb7EbblVZpQokoBK3yIn_UFeOE2Kn0LcnCDiNRvpmNJopGvnGA0JnlFxSkvH5rpjX-x1ZyBGaEMJokjHxNkbTEHY_lnF-jMZUSsWYIhP0snGx0wF8sNHuAV8_LnG5te0VfgcPGL7aDkKwjcdt11TWAdZeu0OwAX_auG36iJ-HiK5bZytb6jigJ-io0i7A6Z_O0Ob25nV1n6yf7h5Wy3VSilTGxBhKFqksjDYplbwgItULkilBBGfSkNRUTGgohFLAB4aZIjOGV1oJWhpm2Axd_PYO0z56CDGvbSjBOe2h6UMuVcalyLIBPP8D-6IGk7edrXV3yP9vYN_6AF93 |
| CitedBy_id | crossref_primary_10_1089_jir_2014_0096 crossref_primary_10_1186_s13287_025_04559_0 crossref_primary_10_1016_j_jbiosc_2012_01_013 crossref_primary_10_1016_j_bbrc_2017_11_164 crossref_primary_10_1016_j_jviromet_2009_07_024 crossref_primary_10_1016_j_jsbmb_2025_106747 crossref_primary_10_1007_s11748_008_0406_9 crossref_primary_10_1007_s00253_009_2209_z crossref_primary_10_1007_s11033_011_1441_7 crossref_primary_10_1038_s41598_020_59939_8 crossref_primary_10_1093_jb_mvq087 crossref_primary_10_1167_iovs_61_11_4 crossref_primary_10_2133_dmpk_DMPK_10_RG_099 crossref_primary_10_1007_s00253_008_1680_2 crossref_primary_10_1128_JCM_00377_08 crossref_primary_10_1002_jat_1622 crossref_primary_10_1016_j_ab_2014_11_012 crossref_primary_10_1016_j_tiv_2009_08_030 crossref_primary_10_1002_lary_24176 crossref_primary_10_1038_bjc_2012_326 crossref_primary_10_1002_gcc_20837 crossref_primary_10_1186_1471_2105_10_411 crossref_primary_10_1038_s41598_017_06137_8 crossref_primary_10_1038_bjc_2014_451 crossref_primary_10_1016_j_bbrc_2011_11_072 crossref_primary_10_1051_vetres_2007042 crossref_primary_10_1038_bjc_2011_588 crossref_primary_10_1186_s12885_024_12676_x crossref_primary_10_4049_jimmunol_1000195 crossref_primary_10_1016_j_clinbiochem_2009_06_005 crossref_primary_10_1371_journal_pone_0031397 crossref_primary_10_1038_bjc_2015_366 crossref_primary_10_1271_bbb_120817 crossref_primary_10_1016_j_mam_2019_10_002 |
| ContentType | Journal Article |
| DBID | CGR CUY CVF ECM EIF NPM 7X8 |
| DOI | 10.1093/jb/mvj086 |
| DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic |
| DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic |
| DatabaseTitleList | MEDLINE - Academic MEDLINE |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: 7X8 name: MEDLINE - Academic url: https://search.proquest.com/medline sourceTypes: Aggregation Database |
| DeliveryMethod | no_fulltext_linktorsrc |
| Discipline | Anatomy & Physiology |
| ExternalDocumentID | 16672270 |
| Genre | Research Support, Non-U.S. Gov't Journal Article |
| GroupedDBID | --- -E4 -~X .2P .55 .CO .GJ .I3 0R~ 18M 1TH 29J 2WC 3O- 4.4 482 48X 53G 5GY 5RE 5WA 5WD 6.Y 70D A8Z AAIMJ AAJKP AAJQQ AAMDB AAMVS AAOGV AAPQZ AAPXW AARHZ AAUAY AAUQX AAVAP AAVLN AAWDT AAYJJ ABDBF ABDFA ABEFU ABEJV ABEUO ABGNP ABIXL ABJNI ABMNT ABNGD ABNKS ABPTD ABQLI ABQTQ ABSAR ABSMQ ABVGC ABWST ABXVV ABZBJ ACFRR ACGFO ACGFS ACIWK ACNCT ACPQN ACPRK ACUFI ACUHS ACUTJ ACZBC ADBBV ADEYI ADEZT ADFTL ADGKP ADGZP ADHKW ADHZD ADIPN ADOCK ADQBN ADRIX ADRTK ADVEK ADYVW ADZTZ ADZXQ AEGPL AEGXH AEHUL AEJOX AEKPW AEKSI AELWJ AEMDU AENEX AENZO AEPUE AETBJ AEWNT AFFNX AFFZL AFGWE AFIYH AFOFC AFRAH AFSHK AFXEN AFYAG AGINJ AGKEF AGKRT AGMDO AGQXC AGSYK AHXPO AI. AIAGR AIJHB AJEEA AKHUL AKWXX ALMA_UNASSIGNED_HOLDINGS ALUQC ANFBD APIBT APJGH APWMN AQDSO ARIXL ASAOO ASPBG ATDFG ATGXG ATTQO AVWKF AXUDD AYOIW AZFZN BAYMD BCRHZ BEYMZ BHONS BQDIO BSWAC C1A C45 CAG CDBKE CGR COF CS3 CUY CVF CXTWN CZ4 DAKXR DFGAJ DILTD D~K EBD EBS ECM EE~ EIF EJD ELUNK EMOBN ESX F5P F9B FEDTE FHSFR FLUFQ FOEOM FQBLK GAUVT GJXCC H13 H5~ HAR HH5 HVGLF HW0 HZ~ H~9 IOX J21 JSF JSH KAQDR KBUDW KOP KQ8 KSI KSN L7B M-Z M49 MBTAY MVM N9A NGC NLBLG NOMLY NPM NTWIH NU- NVLIB O0~ O9- OAWHX OBOKY ODMLO OJQWA OJZSN OK1 OVD OWPYF O~Y P2P PAFKI PB- PEELM PQQKQ Q1. Q5Y QBD R44 RD5 RJT RNI ROL ROX ROZ RUSNO RW1 RXO RZF RZJ RZO SJN SV3 TCN TEORI TKC TLC TN5 TUS TWZ UQL VH1 WH7 WHG X7H X7J X7M XJT Y6R YAYTL YKOAZ YROCO YXANX Z5M ZE2 ZGI ZKB ZKX ZXP ~91 ~KM 7X8 AAILS ABPQP ABXZS ACUKT ADCFL ADNBA AEHKS AGQPQ AJBYB AJNCP ALXQX ESTFP JXSIZ |
| ID | FETCH-LOGICAL-c456t-dd10856bdad5163b045a8097404326d05df24aeb477e3ad52db9dd3fa741cd2d2 |
| IEDL.DBID | 7X8 |
| ISICitedReferencesCount | 45 |
| ISICitedReferencesURI | http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000237887000009&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| ISSN | 0021-924X |
| IngestDate | Sat Sep 27 22:30:59 EDT 2025 Wed Feb 19 01:53:51 EST 2025 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 4 |
| Language | English |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c456t-dd10856bdad5163b045a8097404326d05df24aeb477e3ad52db9dd3fa741cd2d2 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| PMID | 16672270 |
| PQID | 67936499 |
| PQPubID | 23479 |
| ParticipantIDs | proquest_miscellaneous_67936499 pubmed_primary_16672270 |
| PublicationCentury | 2000 |
| PublicationDate | 2006-04-01 |
| PublicationDateYYYYMMDD | 2006-04-01 |
| PublicationDate_xml | – month: 04 year: 2006 text: 2006-04-01 day: 01 |
| PublicationDecade | 2000 |
| PublicationPlace | England |
| PublicationPlace_xml | – name: England |
| PublicationTitle | Journal of biochemistry (Tokyo) |
| PublicationTitleAlternate | J Biochem |
| PublicationYear | 2006 |
| SSID | ssj0021233 |
| Score | 2.1073701 |
| Snippet | We have developed a new DNA chip whose substrate has a unique minute columnar array structure made of plastic. The DNA chip exhibits ultrahigh sensitivity, up... |
| SourceID | proquest pubmed |
| SourceType | Aggregation Database Index Database |
| StartPage | 697 |
| SubjectTerms | Gene Expression Profiling - instrumentation Gene Expression Profiling - methods Microscopy, Electron, Scanning Nucleic Acid Amplification Techniques - methods Nucleic Acid Hybridization - methods Oligonucleotide Array Sequence Analysis - instrumentation Oligonucleotide Array Sequence Analysis - methods Plasminogen Activators - genetics Reproducibility of Results Reverse Transcriptase Polymerase Chain Reaction - methods RNA - genetics RNA - metabolism RNA, Messenger - genetics RNA, Messenger - metabolism Statistics, Nonparametric |
| Title | Ultrasensitive DNA chip: gene expression profile analysis without RNA amplification |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/16672270 https://www.proquest.com/docview/67936499 |
| Volume | 139 |
| WOSCitedRecordID | wos000237887000009&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| hasFullText | |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV07T8MwELYKZWDhVR7l6QGxRU2cxE4QEqqAioWo4iFli-zYFq1oUtq0gn_POQ-YEAOLJzuKTnfn7-4-3yF0LmzCwY6opQMVWp6kYFI-4RYLpQ6ZlhBE83LYBIuiII7DYQtdNW9hDK2y8Ymlo5Z5anLkPQqKRAGeX0_fLTMzytRW6wEaK6jtApAxZsni7xqC8clVfdmQEIgXN32FQrc3Fr3JcmwH9HdcWd4vg83__dkW2qhxJe5XirCNWirbQZ1-BjH15BNf4JLpWabQO-jp5a2Ywe2VzUviEL6N-jh9HU0vMWiTwuqjJsdmuJ7ojXndugSbtG2-KPAjHOGGjK7rnN8uehncPd_cW_VwBSsFzFRYUpp3B1RILn3AZAKgHQ9siC5Mjz4qbV9q4nElPMaUC3uIFKGUruYAQVJJJNlDq1meqQOEGXXgG45ta4g2pabcS11XCZ8r6jupUF101gguAeU1FQmeqXwxTxrRddF-JftkWvXYSBxKGSHMPvzz7BFa_0mLHKO2BrNVJ2gtXRaj-ey01AlYo-HDF1Exw8s |
| linkProvider | ProQuest |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Ultrasensitive+DNA+chip%3A+gene+expression+profile+analysis+without+RNA+amplification&rft.jtitle=Journal+of+biochemistry+%28Tokyo%29&rft.au=Nagino%2C+Kunihisa&rft.au=Nomura%2C+Osamu&rft.au=Takii%2C+Yuki&rft.au=Myomoto%2C+Akira&rft.date=2006-04-01&rft.issn=0021-924X&rft.volume=139&rft.issue=4&rft.spage=697&rft_id=info:doi/10.1093%2Fjb%2Fmvj086&rft.externalDBID=NO_FULL_TEXT |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0021-924X&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0021-924X&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0021-924X&client=summon |