Increased frequency (12%) of circulating chronic lymphocytic leukemia-like B-cell clones in healthy subjects using a highly sensitive multicolor flow cytometry approach
Monoclonal B-cell lymphocytosis (MBL) indicates the presence of less than 5 x 10(9)/L circulating monoclonal B cells in otherwise healthy subjects. Recently, it has been reported that circulating chronic lymphocytic leukemia (CLL)-like B cells can be detected using 4- or 5-multicolor flow cytometry...
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| Vydáno v: | Blood Ročník 114; číslo 1; s. 33 |
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| Hlavní autoři: | , , , , , , , , , , , , |
| Médium: | Journal Article |
| Jazyk: | angličtina |
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02.07.2009
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| ISSN: | 1528-0020, 1528-0020 |
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| Abstract | Monoclonal B-cell lymphocytosis (MBL) indicates the presence of less than 5 x 10(9)/L circulating monoclonal B cells in otherwise healthy subjects. Recently, it has been reported that circulating chronic lymphocytic leukemia (CLL)-like B cells can be detected using 4- or 5-multicolor flow cytometry in 5% to 7% of adults with normal lymphocyte counts. We investigated the frequency of circulating monoclonal B cells in 608 healthy subjects older than 40 years with normal blood counts, using a highly sensitive 8-color flow cytometry approach and systematic screening for total PB leukocyte count higher than 5 x 10(6). We show that the frequency of PB monoclonal B cells is markedly higher than previously reported (12% for CLL-like B cells, found at frequencies of 0.17 +/- 0.13 x 10(9) cells/L), the incidence progressively increasing with age. Most cases (62%) showed clonal B-cell levels below the maximum sensitivity of the techniques described by others (< 0.01%), supporting the notion that detection of MBL may largely depend on the sensitivity of the flow cytometry approach used. |
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| AbstractList | Monoclonal B-cell lymphocytosis (MBL) indicates the presence of less than 5 x 10(9)/L circulating monoclonal B cells in otherwise healthy subjects. Recently, it has been reported that circulating chronic lymphocytic leukemia (CLL)-like B cells can be detected using 4- or 5-multicolor flow cytometry in 5% to 7% of adults with normal lymphocyte counts. We investigated the frequency of circulating monoclonal B cells in 608 healthy subjects older than 40 years with normal blood counts, using a highly sensitive 8-color flow cytometry approach and systematic screening for total PB leukocyte count higher than 5 x 10(6). We show that the frequency of PB monoclonal B cells is markedly higher than previously reported (12% for CLL-like B cells, found at frequencies of 0.17 +/- 0.13 x 10(9) cells/L), the incidence progressively increasing with age. Most cases (62%) showed clonal B-cell levels below the maximum sensitivity of the techniques described by others (< 0.01%), supporting the notion that detection of MBL may largely depend on the sensitivity of the flow cytometry approach used. Monoclonal B-cell lymphocytosis (MBL) indicates the presence of less than 5 x 10(9)/L circulating monoclonal B cells in otherwise healthy subjects. Recently, it has been reported that circulating chronic lymphocytic leukemia (CLL)-like B cells can be detected using 4- or 5-multicolor flow cytometry in 5% to 7% of adults with normal lymphocyte counts. We investigated the frequency of circulating monoclonal B cells in 608 healthy subjects older than 40 years with normal blood counts, using a highly sensitive 8-color flow cytometry approach and systematic screening for total PB leukocyte count higher than 5 x 10(6). We show that the frequency of PB monoclonal B cells is markedly higher than previously reported (12% for CLL-like B cells, found at frequencies of 0.17 +/- 0.13 x 10(9) cells/L), the incidence progressively increasing with age. Most cases (62%) showed clonal B-cell levels below the maximum sensitivity of the techniques described by others (< 0.01%), supporting the notion that detection of MBL may largely depend on the sensitivity of the flow cytometry approach used.Monoclonal B-cell lymphocytosis (MBL) indicates the presence of less than 5 x 10(9)/L circulating monoclonal B cells in otherwise healthy subjects. Recently, it has been reported that circulating chronic lymphocytic leukemia (CLL)-like B cells can be detected using 4- or 5-multicolor flow cytometry in 5% to 7% of adults with normal lymphocyte counts. We investigated the frequency of circulating monoclonal B cells in 608 healthy subjects older than 40 years with normal blood counts, using a highly sensitive 8-color flow cytometry approach and systematic screening for total PB leukocyte count higher than 5 x 10(6). We show that the frequency of PB monoclonal B cells is markedly higher than previously reported (12% for CLL-like B cells, found at frequencies of 0.17 +/- 0.13 x 10(9) cells/L), the incidence progressively increasing with age. Most cases (62%) showed clonal B-cell levels below the maximum sensitivity of the techniques described by others (< 0.01%), supporting the notion that detection of MBL may largely depend on the sensitivity of the flow cytometry approach used. |
| Author | Jara-Acevedo, María González, Marcos López, Antonio Sánchez, Maria Luz Romero, Alfonso Vega, Tomás Fernández-Navarro, Paulino Henriques, Ana F Rasillo, Ana Orfao, Alberto Almeida, Julia Teodosio, Cristina Nieto, Wendy G |
| Author_xml | – sequence: 1 givenname: Wendy G surname: Nieto fullname: Nieto, Wendy G organization: Instituto de Biología Molecular y Celular del Cáncer, Centro de Investigación del Cáncer/IBMCC (CSIC-USAL), Department of Medicine, University of Salamanca, Salamanca, Spain – sequence: 2 givenname: Julia surname: Almeida fullname: Almeida, Julia – sequence: 3 givenname: Alfonso surname: Romero fullname: Romero, Alfonso – sequence: 4 givenname: Cristina surname: Teodosio fullname: Teodosio, Cristina – sequence: 5 givenname: Antonio surname: López fullname: López, Antonio – sequence: 6 givenname: Ana F surname: Henriques fullname: Henriques, Ana F – sequence: 7 givenname: Maria Luz surname: Sánchez fullname: Sánchez, Maria Luz – sequence: 8 givenname: María surname: Jara-Acevedo fullname: Jara-Acevedo, María – sequence: 9 givenname: Ana surname: Rasillo fullname: Rasillo, Ana – sequence: 10 givenname: Marcos surname: González fullname: González, Marcos – sequence: 11 givenname: Paulino surname: Fernández-Navarro fullname: Fernández-Navarro, Paulino – sequence: 12 givenname: Tomás surname: Vega fullname: Vega, Tomás – sequence: 13 givenname: Alberto surname: Orfao fullname: Orfao, Alberto |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/19420353$$D View this record in MEDLINE/PubMed |
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| SubjectTerms | Adult Aged Aged, 80 and over B-Lymphocytes - immunology B-Lymphocytes - pathology Diagnosis, Differential Disease Progression Female Flow Cytometry - methods Flow Cytometry - statistics & numerical data Gene Rearrangement, B-Lymphocyte, Heavy Chain Humans Immunophenotyping Leukemia, Lymphocytic, Chronic, B-Cell - blood Leukemia, Lymphocytic, Chronic, B-Cell - diagnosis Leukemia, Lymphocytic, Chronic, B-Cell - genetics Leukemia, Lymphocytic, Chronic, B-Cell - immunology Lymphocyte Count Lymphocytosis - blood Lymphocytosis - diagnosis Lymphocytosis - genetics Lymphocytosis - immunology Male Middle Aged Precancerous Conditions - blood Precancerous Conditions - diagnosis Precancerous Conditions - genetics Precancerous Conditions - immunology Sensitivity and Specificity |
| Title | Increased frequency (12%) of circulating chronic lymphocytic leukemia-like B-cell clones in healthy subjects using a highly sensitive multicolor flow cytometry approach |
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