Evaluation of the validity of a maximum likelihood adaptive staircase procedure for measurement of olfactory detection threshold in mice

Threshold is defined as the stimulus intensity necessary for a subject to reach a specified percent correct on a detection test. MLPEST (maximum likelihood parameter estimation by sequential testing) is a method that is able to determine threshold accurately and more rapidly than many other methods....

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Vydané v:Chemical senses Ročník 31; číslo 1; s. 9
Hlavní autori: Clevenger, Amy C, Restrepo, Diego
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: England 01.01.2006
Predmet:
Animals
Computer Simulation
Cyclic Nucleotide-Gated Cation Channels
Diagnostic Techniques and Procedures
Ion Channels - deficiency
Mice
Mice, Knockout
Monte Carlo Method
Odorants - analysis
Olfaction Disorders - diagnosis
Olfactory Pathways - physiology
Psychomotor Performance
Sensory Thresholds
ISSN:0379-864X
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Shrnutí:Threshold is defined as the stimulus intensity necessary for a subject to reach a specified percent correct on a detection test. MLPEST (maximum likelihood parameter estimation by sequential testing) is a method that is able to determine threshold accurately and more rapidly than many other methods. Originally developed for human auditory and visual tasks, it has been adapted for human olfactory and gustatory tests. In order to utilize this technique for olfactory testing in mice, we have adapted MLPEST methodology for use with computerized olfactometry as a tool to estimate odor detection thresholds. Here we present Monte Carlo simulations and operant conditioning data that demonstrate the potential utility of this technique in mice, we explore the ramifications of altering MLPEST test parameters on performance, and we discuss the advantages and disadvantages of using MLPEST compared to other methods for the estimation of thresholds in rodents. Using MLPEST, we find that olfactory detection thresholds in mice deficient for the cyclic nucleotide-gated channel subunit A2 are similar to those of wild-type animals for odorants the knockout animals are able to detect.
Bibliografia:ObjectType-Article-2
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ISSN:0379-864X
DOI:10.1093/chemse/bjj001