Increased amounts of laminin in GCF from untreated patients with periodontitis

Background/aims: Our aim was to compare the levels of laminin and interleukin‐8 (IL‐8) in GCF from inflamed shallow (GP) and deep pockets (PP) in patients with periodontitis to these levels in GCF from inflamed shallow pockets (GG) in subjects with gingivitis alone. Method: Lactoferrin was used as a...

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Vydáno v:Journal of clinical periodontology Ročník 27; číslo 5; s. 313 - 318
Hlavní autoři: Figueredo, C. M. S., Gustafsson, A.
Médium: Journal Article
Jazyk:angličtina
Vydáno: Copenhagen Munksgaard International Publishers 01.05.2000
Témata:
Adult
Basement Membrane - metabolism
Chemotaxis, Leukocyte
Female
GCF
Gingival Crevicular Fluid - chemistry
Humans
interleukin-8
Interleukin-8 - analysis
lactoferrin
Lactoferrin - analysis
Lactoferrin - metabolism
laminin
Laminin - analysis
Laminin - metabolism
laminin, interleukin‐8
Male
Neutrophil Activation
periodontitis
Periodontitis - immunology
Periodontitis - metabolism
Statistics, Nonparametric
ISSN:0303-6979, 1600-051X
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Shrnutí:Background/aims: Our aim was to compare the levels of laminin and interleukin‐8 (IL‐8) in GCF from inflamed shallow (GP) and deep pockets (PP) in patients with periodontitis to these levels in GCF from inflamed shallow pockets (GG) in subjects with gingivitis alone. Method: Lactoferrin was used as a marker for the number of neutrophils in the sites sampled. The periodontitis group consisted of 13 subjects, having at least 6 sites with a pocket depth 5 mm. The healthy control group consisted of 12 subjects with no clinical signs of periodontal destruction. GCF was collected with paper strips and the volume was measured immediately after sampling. Laminin, lactoferrin and IL‐8 were measured using specific antibodies with an ELISA. Results: The total amount of laminin was significantly higher in PP than in GG, but no significant difference was seen between GP and PP. The concentration and the total amounts of lactoferrin were similar in the three groups. The ratio between laminin and lactoferrin was higher in the samples from the patient, suggesting that neutrophils in patients are more activated and degrades more of the membrane per cell. The total amounts of IL‐8 were very similar in the 3 groups, while the concentration also tended to be higher in the GP. Conclusions: Our study showed higher amounts of laminin in GCF from patients with periodontitis suggesting the presence of hyperactive neutrophils during the transmigration process through the endothelium/epithelium.
Bibliografie:ark:/67375/WNG-JK1LP9G1-D
ArticleID:JCPE270504
istex:A043D66284D60B7694BD74704057F7C630310AEE
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0303-6979
1600-051X
DOI:10.1034/j.1600-051x.2000.027005313.x