Altered mitochondrial DNA methylation and mitochondrial DNA copy number in an APP/PS1 transgenic mouse model of Alzheimer disease

Alzheimer’s disease (AD) is a chronic neurodegenerative disease and mitochondrial impairment is a key feature of AD. The mitochondrial DNA (mtDNA) epigenetic mechanism is a relatively new field compared to nuclear DNA. The relationship between mtDNA epigenetic mechanism and AD hasn’t been establishe...

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Vydáno v:Biochemical and biophysical research communications Ročník 520; číslo 1; s. 41 - 46
Hlavní autoři: Xu, YingYing, Xu, LinLin, Han, Min, Liu, XiangTian, Li, Fan, Zhou, XiaoYan, Wang, Yun, Bi, JianZhong
Médium: Journal Article
Jazyk:angličtina
Vydáno: United States Elsevier Inc 26.11.2019
Témata:
12S rRNA gene
Alzheimer disease
Alzheimer’s disease
amyloid
APP/PS1 transgenic mice
bisulfites
D-loop
DNA methylation
epigenetics
gene expression
high-throughput nucleotide sequencing
mice
mitochondria
mitochondrial DNA
Mitochondrial DNA copy number
Mitochondrial DNA methylation
mitochondrial genes
neurodegenerative diseases
nuclear genome
quantitative polymerase chain reaction
Mitochondrial DNA copy number
APP/PS1 transgenic mice
D-loop
Alzheimer’s disease
12S rRNA gene
Mitochondrial DNA methylation
ISSN:0006-291X, 1090-2104, 1090-2104
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Shrnutí:Alzheimer’s disease (AD) is a chronic neurodegenerative disease and mitochondrial impairment is a key feature of AD. The mitochondrial DNA (mtDNA) epigenetic mechanism is a relatively new field compared to nuclear DNA. The relationship between mtDNA epigenetic mechanism and AD hasn’t been established. So we analyzed the mtDNA methylation in D-loop region and 12 S rRNA gene in the hippocampi in amyloid precursor protein/presenilin 1 (APP/PS1) transgenic mice by bisulfite pyrosequencing. Mitochondrial DNA copy number and gene expression were studied by quantitative real-time PCR (qRT-PCR). We observed a decrease in the displacement loop (D-loop) methylation and an increase in 12 S rRNA gene methylation, while both the mtDNA copy number and the mitochondrial gene expression were reduced in APP/PS1 transgenic mice. In summary, the present finding suggest that mtDNA methylation may play a role in AD pathology, which warrants larger future investigations. •We analyzed the mtDNA methylation in D-loop region and 12 S rRNA gene in the hippocampi in APP/PS1 transgenic mice by bisulfite pyrosequencing.•There were only two studies performed so far to investigate mtDNA D-loop methylation in AD.•And this is the first study to demonstrate a link between the hypermethylation of 12 S rRNA gene and AD.
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ISSN:0006-291X
1090-2104
1090-2104
DOI:10.1016/j.bbrc.2019.09.094