TOP-LD: A tool to explore linkage disequilibrium with TOPMed whole-genome sequence data

Current publicly available tools that allow rapid exploration of linkage disequilibrium (LD) between markers (e.g., HaploReg and LDlink) are based on whole-genome sequence (WGS) data from 2,504 individuals in the 1000 Genomes Project. Here, we present TOP-LD, an online tool to explore LD inferred wi...

Celý popis

Uloženo v:

Podrobná bibliografie
Vydáno v:	American journal of human genetics Ročník 109; číslo 6; s. 1175
Hlavní autoři:	Huang, Le, Rosen, Jonathan D, Sun, Quan, Chen, Jiawen, Wheeler, Marsha M, Zhou, Ying, Min, Yuan-I, Kooperberg, Charles, Conomos, Matthew P, Stilp, Adrienne M, Rich, Stephen S, Rotter, Jerome I, Manichaikul, Ani, Loos, Ruth J F, Kenny, Eimear E, Blackwell, Thomas W, Smith, Albert V, Jun, Goo, Sedlazeck, Fritz J, Metcalf, Ginger, Boerwinkle, Eric, Raffield, Laura M, Reiner, Alex P, Auer, Paul L, Li, Yun
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	United States 02.06.2022
Témata:	Asian People Genome-Wide Association Study Humans Linkage Disequilibrium - genetics Polymorphism, Single Nucleotide - genetics Precision Medicine Whole Genome Sequencing
ISSN:	1537-6605, 1537-6605
On-line přístup:	Zjistit podrobnosti o přístupu
Tagy:	Přidat tag Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!

Abstract	Current publicly available tools that allow rapid exploration of linkage disequilibrium (LD) between markers (e.g., HaploReg and LDlink) are based on whole-genome sequence (WGS) data from 2,504 individuals in the 1000 Genomes Project. Here, we present TOP-LD, an online tool to explore LD inferred with high-coverage (∼30×) WGS data from 15,578 individuals in the NHLBI Trans-Omics for Precision Medicine (TOPMed) program. TOP-LD provides a significant upgrade compared to current LD tools, as the TOPMed WGS data provide a more comprehensive representation of genetic variation than the 1000 Genomes data, particularly for rare variants and in the specific populations that we analyzed. For example, TOP-LD encompasses LD information for 150.3, 62.2, and 36.7 million variants for European, African, and East Asian ancestral samples, respectively, offering 2.6- to 9.1-fold increase in variant coverage compared to HaploReg 4.0 or LDlink. In addition, TOP-LD includes tens of thousands of structural variants (SVs). We demonstrate the value of TOP-LD in fine-mapping at the GGT1 locus associated with gamma glutamyltransferase in the African ancestry participants in UK Biobank. Beyond fine-mapping, TOP-LD can facilitate a wide range of applications that are based on summary statistics and estimates of LD. TOP-LD is freely available online.
AbstractList	Current publicly available tools that allow rapid exploration of linkage disequilibrium (LD) between markers (e.g., HaploReg and LDlink) are based on whole-genome sequence (WGS) data from 2,504 individuals in the 1000 Genomes Project. Here, we present TOP-LD, an online tool to explore LD inferred with high-coverage (∼30×) WGS data from 15,578 individuals in the NHLBI Trans-Omics for Precision Medicine (TOPMed) program. TOP-LD provides a significant upgrade compared to current LD tools, as the TOPMed WGS data provide a more comprehensive representation of genetic variation than the 1000 Genomes data, particularly for rare variants and in the specific populations that we analyzed. For example, TOP-LD encompasses LD information for 150.3, 62.2, and 36.7 million variants for European, African, and East Asian ancestral samples, respectively, offering 2.6- to 9.1-fold increase in variant coverage compared to HaploReg 4.0 or LDlink. In addition, TOP-LD includes tens of thousands of structural variants (SVs). We demonstrate the value of TOP-LD in fine-mapping at the GGT1 locus associated with gamma glutamyltransferase in the African ancestry participants in UK Biobank. Beyond fine-mapping, TOP-LD can facilitate a wide range of applications that are based on summary statistics and estimates of LD. TOP-LD is freely available online. Current publicly available tools that allow rapid exploration of linkage disequilibrium (LD) between markers (e.g., HaploReg and LDlink) are based on whole-genome sequence (WGS) data from 2,504 individuals in the 1000 Genomes Project. Here, we present TOP-LD, an online tool to explore LD inferred with high-coverage (∼30×) WGS data from 15,578 individuals in the NHLBI Trans-Omics for Precision Medicine (TOPMed) program. TOP-LD provides a significant upgrade compared to current LD tools, as the TOPMed WGS data provide a more comprehensive representation of genetic variation than the 1000 Genomes data, particularly for rare variants and in the specific populations that we analyzed. For example, TOP-LD encompasses LD information for 150.3, 62.2, and 36.7 million variants for European, African, and East Asian ancestral samples, respectively, offering 2.6- to 9.1-fold increase in variant coverage compared to HaploReg 4.0 or LDlink. In addition, TOP-LD includes tens of thousands of structural variants (SVs). We demonstrate the value of TOP-LD in fine-mapping at the GGT1 locus associated with gamma glutamyltransferase in the African ancestry participants in UK Biobank. Beyond fine-mapping, TOP-LD can facilitate a wide range of applications that are based on summary statistics and estimates of LD. TOP-LD is freely available online.Current publicly available tools that allow rapid exploration of linkage disequilibrium (LD) between markers (e.g., HaploReg and LDlink) are based on whole-genome sequence (WGS) data from 2,504 individuals in the 1000 Genomes Project. Here, we present TOP-LD, an online tool to explore LD inferred with high-coverage (∼30×) WGS data from 15,578 individuals in the NHLBI Trans-Omics for Precision Medicine (TOPMed) program. TOP-LD provides a significant upgrade compared to current LD tools, as the TOPMed WGS data provide a more comprehensive representation of genetic variation than the 1000 Genomes data, particularly for rare variants and in the specific populations that we analyzed. For example, TOP-LD encompasses LD information for 150.3, 62.2, and 36.7 million variants for European, African, and East Asian ancestral samples, respectively, offering 2.6- to 9.1-fold increase in variant coverage compared to HaploReg 4.0 or LDlink. In addition, TOP-LD includes tens of thousands of structural variants (SVs). We demonstrate the value of TOP-LD in fine-mapping at the GGT1 locus associated with gamma glutamyltransferase in the African ancestry participants in UK Biobank. Beyond fine-mapping, TOP-LD can facilitate a wide range of applications that are based on summary statistics and estimates of LD. TOP-LD is freely available online.
Author	Huang, Le Rich, Stephen S Min, Yuan-I Wheeler, Marsha M Raffield, Laura M Li, Yun Sedlazeck, Fritz J Rotter, Jerome I Loos, Ruth J F Boerwinkle, Eric Rosen, Jonathan D Sun, Quan Stilp, Adrienne M Chen, Jiawen Reiner, Alex P Blackwell, Thomas W Metcalf, Ginger Kenny, Eimear E Auer, Paul L Kooperberg, Charles Smith, Albert V Zhou, Ying Manichaikul, Ani Jun, Goo Conomos, Matthew P
Author_xml	– sequence: 1 givenname: Le surname: Huang fullname: Huang, Le organization: Curriculum in Bioinformatics and Computational Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA – sequence: 2 givenname: Jonathan D surname: Rosen fullname: Rosen, Jonathan D organization: Department of Biostatistics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA – sequence: 3 givenname: Quan surname: Sun fullname: Sun, Quan organization: Department of Biostatistics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA – sequence: 4 givenname: Jiawen surname: Chen fullname: Chen, Jiawen organization: Department of Biostatistics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA – sequence: 5 givenname: Marsha M surname: Wheeler fullname: Wheeler, Marsha M organization: Department of Genome Sciences, University of Washington, Seattle, WA 98105, USA – sequence: 6 givenname: Ying surname: Zhou fullname: Zhou, Ying organization: Division of Public Health Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA – sequence: 7 givenname: Yuan-I surname: Min fullname: Min, Yuan-I organization: Department of Medicine, University of Mississippi Medical Center, Jackson, MS 39216, USA – sequence: 8 givenname: Charles surname: Kooperberg fullname: Kooperberg, Charles organization: Division of Public Health Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA – sequence: 9 givenname: Matthew P surname: Conomos fullname: Conomos, Matthew P organization: Department of Biostatistics, University of Washington, Seattle, WA 98105, USA – sequence: 10 givenname: Adrienne M surname: Stilp fullname: Stilp, Adrienne M organization: Department of Biostatistics, University of Washington, Seattle, WA 98105, USA – sequence: 11 givenname: Stephen S surname: Rich fullname: Rich, Stephen S organization: Center for Public Health Genomics, Department of Public Health Sciences, University of Virginia School of Medicine, Charlottesville, VA 22908, USA – sequence: 12 givenname: Jerome I surname: Rotter fullname: Rotter, Jerome I organization: The Institute for Translational Genomics and Population Sciences, Department of Pediatrics, The Lundquist Institute for Biomedical Innovation at Harbor-UCLA Medical Center, Torrance, CA 90502, USA – sequence: 13 givenname: Ani surname: Manichaikul fullname: Manichaikul, Ani organization: Center for Public Health Genomics, Department of Public Health Sciences, University of Virginia School of Medicine, Charlottesville, VA 22908, USA – sequence: 14 givenname: Ruth J F surname: Loos fullname: Loos, Ruth J F organization: The Charles Bronfman Institute for Personalized Medicine, Icahn School of Medicine at Mount Sinai, New York City, NY 10029, USA; Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark – sequence: 15 givenname: Eimear E surname: Kenny fullname: Kenny, Eimear E organization: The Charles Bronfman Institute for Personalized Medicine, Icahn School of Medicine at Mount Sinai, New York City, NY 10029, USA – sequence: 16 givenname: Thomas W surname: Blackwell fullname: Blackwell, Thomas W organization: TOPMed Informatics Research Center, University of Michigan, Department of Biostatistics, Ann Arbor, MI 48109, USA – sequence: 17 givenname: Albert V surname: Smith fullname: Smith, Albert V organization: TOPMed Informatics Research Center, University of Michigan, Department of Biostatistics, Ann Arbor, MI 48109, USA – sequence: 18 givenname: Goo surname: Jun fullname: Jun, Goo organization: Human Genetics Center, School of Public Health, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA – sequence: 19 givenname: Fritz J surname: Sedlazeck fullname: Sedlazeck, Fritz J organization: Human Genome Sequencing Center, Baylor College of Medicine, Houston, TX 77030, USA – sequence: 20 givenname: Ginger surname: Metcalf fullname: Metcalf, Ginger organization: Human Genome Sequencing Center, Baylor College of Medicine, Houston, TX 77030, USA – sequence: 21 givenname: Eric surname: Boerwinkle fullname: Boerwinkle, Eric organization: Human Genetics Center, Department of Epidemiology, Human Genetics, and Environmental Sciences, School of Public Health, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA – sequence: 22 givenname: Laura M surname: Raffield fullname: Raffield, Laura M organization: Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA – sequence: 23 givenname: Alex P surname: Reiner fullname: Reiner, Alex P organization: Department of Epidemiology, University of Washington, Seattle, WA 98195, USA; Division of Public Health Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA – sequence: 24 givenname: Paul L surname: Auer fullname: Auer, Paul L email: pauer@mcw.edu organization: Division of Biostatistics, Institute for Health and Equity, and Cancer Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA. Electronic address: pauer@mcw.edu – sequence: 25 givenname: Yun surname: Li fullname: Li, Yun email: yunli@med.unc.edu organization: Department of Biostatistics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA; Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA; Department of Computer Science, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. Electronic address: yunli@med.unc.edu
BackLink	https://www.ncbi.nlm.nih.gov/pubmed/35504290$$D View this record in MEDLINE/PubMed
BookMark	eNpNkLtOw0AQRVcIRB7wAxRoSxqb2adtuig8paBQBFFau-tx4mB7gx8K_D1GBInmzi3OmZFmQo5rXyMhFwxCBkxfb0Oz3axDDpyHIEMAfUTGTIko0BrU8b8-IpO23QIwFoM4JSOhFEiewJi8rZYvweL2hs5o5305BMXPXekbpGVRv5s10qxo8aMvysI2RV_RfdFt6GA9Y0b3G19isMbaV0h_KKzdIJjOnJGT3JQtnh_mlLze363mj8Fi-fA0ny0CJ5XqAsbRSWtzwxzEmRPWiURaSKIY0fIoV1EODpiIYhnlUiWCgbNCm8wI6axRfEqufvfuGj-cb7u0KlqHZWlq9H2bcq0SDZorPqCXB7S3FWbprikq03ylf8_g3_soY3o
CitedBy_id	crossref_primary_10_1038_s41467_025_62463_w crossref_primary_10_1016_j_ajhg_2022_09_009 crossref_primary_10_1038_s41380_024_02609_2 crossref_primary_10_1093_hmg_ddaf015 crossref_primary_10_1016_j_atherosclerosis_2024_118621 crossref_primary_10_1126_science_abn2937 crossref_primary_10_7554_eLife_95411 crossref_primary_10_1016_j_ajhg_2025_03_004 crossref_primary_10_1111_trf_17944 crossref_primary_10_1016_j_cjca_2023_07_011 crossref_primary_10_1038_s41467_023_43565_9 crossref_primary_10_7554_eLife_95411_3 crossref_primary_10_1016_j_bcmd_2023_102782 crossref_primary_10_1093_genetics_iyae098 crossref_primary_10_1001_jamanetworkopen_2025_0535 crossref_primary_10_1186_s13059_024_03352_1 crossref_primary_10_1016_S2215_0366_25_00124_5 crossref_primary_10_1038_s43587_024_00762_5 crossref_primary_10_1038_s41467_025_58420_2 crossref_primary_10_1038_s41588_023_01506_8 crossref_primary_10_1186_s12859_025_06119_y crossref_primary_10_1186_s12864_024_11172_7 crossref_primary_10_3389_fcell_2022_957292 crossref_primary_10_1093_nar_gkac954 crossref_primary_10_1016_j_celrep_2025_115805 crossref_primary_10_1038_s41467_024_45135_z crossref_primary_10_1182_blood_2023022596 crossref_primary_10_1186_s13195_023_01244_3 crossref_primary_10_1101_gr_279741_124 crossref_primary_10_1161_JAHA_124_036499 crossref_primary_10_1001_jamadermatol_2023_2217 crossref_primary_10_1038_s41467_024_50309_w crossref_primary_10_1016_j_ajhg_2024_04_001
ContentType	Journal Article
Copyright	Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.
Copyright_xml	– notice: Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.
CorporateAuthor	NHLBI Trans-Omics for Precision Medicine (TOPMed) Consortium
CorporateAuthor_xml	– name: NHLBI Trans-Omics for Precision Medicine (TOPMed) Consortium
DBID	CGR CUY CVF ECM EIF NPM 7X8
DOI	10.1016/j.ajhg.2022.04.006
DatabaseName	Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic
DatabaseTitle	MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic
DatabaseTitleList	MEDLINE MEDLINE - Academic
Database_xml	– sequence: 1 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: 7X8 name: MEDLINE - Academic url: https://search.proquest.com/medline sourceTypes: Aggregation Database
DeliveryMethod	no_fulltext_linktorsrc
Discipline	Biology
EISSN	1537-6605
ExternalDocumentID	35504290
Genre	Research Support, Non-U.S. Gov't Journal Article Research Support, N.I.H., Extramural
GrantInformation_xml	– fundername: NHGRI NIH HHS grantid: U01 HG011720 – fundername: NCATS NIH HHS grantid: KL2 TR002490 – fundername: NHLBI NIH HHS grantid: R01 HL129132 – fundername: Medical Research Council grantid: MC_PC_17228 – fundername: NIDA NIH HHS grantid: U01 DA052713 – fundername: NHLBI NIH HHS grantid: T32 HL129982 – fundername: Medical Research Council grantid: MC_QA137853 – fundername: NHLBI NIH HHS grantid: R01 HL146500 – fundername: NIAMS NIH HHS grantid: U24 AR076730
GroupedDBID	--- --K --Z -~X 0R~ 123 1~5 23M 2WC 4.4 457 4G. 53G 5GY 62- 6J9 7-5 85S AAEDT AAEDW AAFWJ AAKRW AALRI AAMRU AAVLU AAWTL AAXUO ABDGV ABJNI ABMAC ABOCM ACGFO ACGFS ACGOD ACNCT ACPRK ACVFH ADBBV ADCNI ADEZE ADVLN AENEX AEUPX AEXQZ AFPUW AFRAH AFTJW AGCQF AGKMS AHMBA AIGII AITUG AKAPO AKBMS AKRWK AKYEP ALMA_UNASSIGNED_HOLDINGS AMRAJ AOIJS APXCP ASPBG AVWKF AZFZN BAWUL CGR CS3 CUY CVF D0L DIK E3Z EBS ECM ECV EFKBS EIF F5P FCP FDB FEDTE GX1 HVGLF HYE IH2 IHE IXB JIG KQ8 L7B M41 NPM O-L O9- OK1 P2P PQQKQ RNS ROL RPM RPZ SES SJN SSZ TN5 TR2 TWZ UHB UKR UNMZH UPT WH7 ZCA 7X8
ID	FETCH-LOGICAL-c455t-12ec4bbfa1c08dc3bc394b0978eeb27f57f0c0137847f459310cb36ada34cba52
IEDL.DBID	7X8
ISICitedReferencesCount	36
ISICitedReferencesURI	http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000823760900006&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
ISSN	1537-6605
IngestDate	Sat Sep 27 21:46:21 EDT 2025 Mon Jul 21 06:03:39 EDT 2025
IsDoiOpenAccess	false
IsOpenAccess	true
IsPeerReviewed	true
IsScholarly	true
Issue	6
Language	English
License	Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.
LinkModel	DirectLink
MergedId	FETCHMERGED-LOGICAL-c455t-12ec4bbfa1c08dc3bc394b0978eeb27f57f0c0137847f459310cb36ada34cba52
Notes	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
OpenAccessLink	http://www.cell.com/article/S0002929722001549/pdf
PMID	35504290
PQID	2659606252
PQPubID	23479
ParticipantIDs	proquest_miscellaneous_2659606252 pubmed_primary_35504290
PublicationCentury	2000
PublicationDate	2022-06-02
PublicationDateYYYYMMDD	2022-06-02
PublicationDate_xml	– month: 06 year: 2022 text: 2022-06-02 day: 02
PublicationDecade	2020
PublicationPlace	United States
PublicationPlace_xml	– name: United States
PublicationTitle	American journal of human genetics
PublicationTitleAlternate	Am J Hum Genet
PublicationYear	2022
SSID	ssj0011803
Score	2.5627851
Snippet	Current publicly available tools that allow rapid exploration of linkage disequilibrium (LD) between markers (e.g., HaploReg and LDlink) are based on...
SourceID	proquest pubmed
SourceType	Aggregation Database Index Database
StartPage	1175
SubjectTerms	Asian People Genome-Wide Association Study Humans Linkage Disequilibrium - genetics Polymorphism, Single Nucleotide - genetics Precision Medicine Whole Genome Sequencing
Title	TOP-LD: A tool to explore linkage disequilibrium with TOPMed whole-genome sequence data
URI	https://www.ncbi.nlm.nih.gov/pubmed/35504290 https://www.proquest.com/docview/2659606252
Volume	109
WOSCitedRecordID	wos000823760900006&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
hasFullText
inHoldings	1
isFullTextHit
isPrint
link	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1LSwMxEA5qFbz4ftQXEbwGt9nNZutFilo82NpDxd5KHrNasbutbRX_vZPslp4EwUtugTCZzHzzyHyEXJgYkkgDvjTOLYt0IJkWaAy5jSVolUgdWk82IdvtpNerd8qE26Rsq5zbRG-obW5cjvySx8KBbS749WjMHGuUq66WFBrLpBIilHFaLXuLKkIt8czI-KglixG3l59miv4u9fb6gvEh537UaRD_DjG9q2lu_veQW2SjBJm0UWjFNlmCbIesFbST37vkufvYYQ-3V7RBp3n-jgsF34oH1NVz0cJQV7cZzwb-Q8BsSF22luKuFlj65Rh1mZvtOgQ678SmrtN0jzw177o396wkWGAmEmLKahxMpHWqaiZIrAm1Cet4XRhYAgbcMhUyDYybSYguLI1EHaGg0WGsrAojo5Xg-2QlyzM4JBSdodaQxgg3dWR5okEIEAotSGJSG5gqOZ9LrI8K7KoSKoN8NukvZFYlB4XY-6Ni0kYfwZBzmMHRH3Yfk3V3m76Ni5-QSorPF07JqvmcDiYfZ14zcG13Wj_ENMLj
linkProvider	ProQuest
openUrl	ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=TOP-LD%3A+A+tool+to+explore+linkage+disequilibrium+with+TOPMed+whole-genome+sequence+data&rft.jtitle=American+journal+of+human+genetics&rft.au=Huang%2C+Le&rft.au=Rosen%2C+Jonathan+D&rft.au=Sun%2C+Quan&rft.au=Chen%2C+Jiawen&rft.date=2022-06-02&rft.eissn=1537-6605&rft.volume=109&rft.issue=6&rft.spage=1175&rft_id=info:doi/10.1016%2Fj.ajhg.2022.04.006&rft_id=info%3Apmid%2F35504290&rft_id=info%3Apmid%2F35504290&rft.externalDocID=35504290
thumbnail_l	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1537-6605&client=summon
thumbnail_m	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1537-6605&client=summon
thumbnail_s	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1537-6605&client=summon