Detection and typing of common human papillomaviruses among Jordanian patients

The epidemiology of human papillomaviruses (HPVs) genotype distribution of cutaneous warts in Jordanian patients were studied. A total of 200 samples were collected using skin swabs from patients with warts attending the dermatology clinic at the Jordan University Hospital over the period of June 20...

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Vydáno v:	Journal of medical virology Ročník 85; číslo 6; s. 1058 - 1062
Hlavní autoři:	Al Bdour, Suzan, Akkash, Laith, Shehabi, Asem A.
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.06.2013 Wiley Wiley Subscription Services, Inc
Témata:	Adolescent Adult Aged Biological and medical sciences Child Child, Preschool cutaneous warts DNA Primers DNA sequencing DNA, Viral - classification DNA, Viral - genetics Epidemiology Female Fundamental and applied biological sciences. Psychology Genotype & phenotype genotyping human papilloma viruses Human papillomavirus Human viral diseases Humans Infectious diseases Jordan - epidemiology Jordan patients Male Medical sciences Microbiology Middle Aged Miscellaneous Molecular Typing Papillomaviridae - classification Papillomaviridae - genetics Papillomavirus Infections - epidemiology Papillomavirus Infections - pathology Papillomavirus Infections - virology Polymerase Chain Reaction Prevalence Sequence Analysis, DNA Skin - pathology Skin - virology Viral diseases Virology Warts - epidemiology Warts - pathology Warts - virology Jordan Human Skin disease cutaneous warts Typing Wart genotyping Jordan patients Genotype human papilloma viruses Infection Viral disease Papilloma Benign neoplasm Detection
ISSN:	0146-6615, 1096-9071, 1096-9071
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Shrnutí:	The epidemiology of human papillomaviruses (HPVs) genotype distribution of cutaneous warts in Jordanian patients were studied. A total of 200 samples were collected using skin swabs from patients with warts attending the dermatology clinic at the Jordan University Hospital over the period of June 2010 to October 2010. Another 100 control samples were taken from healthy Jordanian individuals with no current or previous history of warts. DNA extraction and sequencing was carried out using PCR with the FAP primer pair to detect HPV DNA, followed by multiple‐type‐specific (Multiplex) PCR combined with DNA sequencing. The prevalence of HPV among Jordanian patients tested with warts diagnosed clinically was 82% (157/192); of these 45% (87/192) were detected by FAP PCR method, and 37% (70/192) were detected by multiplex PCR method. Sequencing of the FAP positive samples shows that HPV 2 was associated with the highest prevalence (36%), followed by HPV 27 (28%) and HPV 57 (21%). A total of 6% of healthy persons were positive for HPV DNA. In conclusion, this study demonstrates that alpha HPV types (HPV 2, HPV 27, and HPV 57) are associated with the most prevalent cutaneous warts in Jordanian patients J. Med. Virol. 85: 1058–1062, 2013. © 2013 Wiley Periodicals, Inc.
Bibliografie:	ArticleID:JMV23519 ark:/67375/WNG-38WDLN2L-J istex:A1E3B0BE9D4A334DF03EA21763378EE2BDD1252B ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 ObjectType-Article-2 ObjectType-Feature-1
ISSN:	0146-6615 1096-9071 1096-9071
DOI:	10.1002/jmv.23519