Standardization of extracellular vesicle measurements by flow cytometry through vesicle diameter approximation

Essentials Platelet extracellular vesicles (EVs) concentrations measured by flow cytometers are incomparable. A model is applied to convert ambiguous scatter units to EV diameter in nanometer. Most included flow cytometers lack the sensitivity to detect EVs of 600 nm and smaller. The model outperfor...

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Bibliographic Details

Published in:	Journal of thrombosis and haemostasis Vol. 16; no. 6; pp. 1236 - 1245
Main Authors:	van der Pol, E., Sturk, A., van Leeuwen, T., Nieuwland, R., Coumans, F., Mobarrez, F., Arkesteijn, G., Wauben, M., Siljander, P. R.‐M., Sánchez‐López, V., Otero‐Candelera, R., Ramón, L. A., Dolz, S., Vila, V., Mackman, N., Geddings, J., Mullier, F., Bailly, N., Han, J.‐Y., Kwaan, H. C., Weiss, I. M., Buzás, E. I., Pállinger, E., Harrison, P., Kraan, J., Hedley, B. D., LazoLangner, A., Enjeti, A., Norris, P. J., Paris, C., Susen, S., Bonnefoy, A., Delorme, I., Chandler, W. L., Hau, C., Aass, H. C. D., Connor, D., Wu, X., Dragovic, R., Uotila, L. M., Lacroix, R., Robert, S.
Format:	Journal Article
Language:	English
Published:	England Elsevier Limited 01.06.2018
Subjects:	blood platelets cell‐derived microparticles exosomes extracellular vesicles Flow cytometry Light scattering Platelets Polystyrene Standardization standardization blood platelets flow cytometry extracellular vesicles cell-derived microparticles exosomes
ISSN:	1538-7933, 1538-7836, 1538-7836
Online Access:	Get full text
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