Flow cytometry isolation and reverse transcriptase-polymerase chain reaction characterization of human round spermatids in infertile patients

Flow cytometry coupled to cell sorting is proposed as a method to isolate round spermatids from testicular biopsies in obstructive azoospermic patients. The cells were separated on the basis of their size and density only. We obtained homogenous populations of alive round spermatids free of lymphocy...

Full description

Saved in:

Bibliographic Details
Published in:	Human reproduction (Oxford) Vol. 14; no. 2; p. 379
Main Authors:	Ziyyat, A, Lassalle, B, Testart, J, Briot, P, Amar, E, Finaz, C, Lefèvre, A
Format:	Journal Article
Language:	English
Published:	England 01.02.1999
Subjects:	Adult Antigens, Neoplasm Biopsy CD3 Complex - metabolism Cell Separation Ejaculation - physiology Flow Cytometry Gene Expression - physiology Humans Infertility, Male - genetics Infertility, Male - metabolism Infertility, Male - pathology Isomerism Male Melanoma-Specific Antigens Middle Aged Neoplasm Proteins - metabolism Protamines - genetics Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - metabolism Spermatids - metabolism Spermatids - pathology Spermatids - physiology Testis - pathology
ISSN:	0268-1161
Online Access:	Get more information
Tags:	Add Tag No Tags, Be the first to tag this record!

Be the first to leave a comment!