Flow cytometry isolation and reverse transcriptase-polymerase chain reaction characterization of human round spermatids in infertile patients

Flow cytometry coupled to cell sorting is proposed as a method to isolate round spermatids from testicular biopsies in obstructive azoospermic patients. The cells were separated on the basis of their size and density only. We obtained homogenous populations of alive round spermatids free of lymphocy...

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Vydáno v:	Human reproduction (Oxford) Ročník 14; číslo 2; s. 379
Hlavní autoři:	Ziyyat, A, Lassalle, B, Testart, J, Briot, P, Amar, E, Finaz, C, Lefèvre, A
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	England 01.02.1999
Témata:	Adult Antigens, Neoplasm Biopsy CD3 Complex - metabolism Cell Separation Ejaculation - physiology Flow Cytometry Gene Expression - physiology Humans Infertility, Male - genetics Infertility, Male - metabolism Infertility, Male - pathology Isomerism Male Melanoma-Specific Antigens Middle Aged Neoplasm Proteins - metabolism Protamines - genetics Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - metabolism Spermatids - metabolism Spermatids - pathology Spermatids - physiology Testis - pathology
ISSN:	0268-1161
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