Transient bursts of Zscan4 expression are accompanied by the rapid derepression of heterochromatin in mouse embryonic stem cells

Mouse embryonic stem cells (mESCs) have a remarkable capacity to maintain normal genome stability and karyotype in culture. We previously showed that infrequent bursts of Zscan4 expression (Z4 events) are important for the maintenance of telomere length and genome stability in mESCs. However, the mo...

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Vydáno v:DNA research Ročník 22; číslo 5; s. 307 - 318
Hlavní autoři: Akiyama, Tomohiko, Xin, Li, Oda, Mayumi, Sharov, Alexei A., Amano, Misa, Piao, Yulan, Cadet, J. Scotty, Dudekula, Dawood B., Qian, Yong, Wang, Weidong, Ko, Shigeru B. H., Ko, Minoru S. H.
Médium: Journal Article
Jazyk:angličtina
Vydáno: England Oxford University Press 01.10.2015
Témata:
Acetylation
Animals
Cell Nucleolus - metabolism
Chromatin Assembly and Disassembly
DNA Methylation
Epigenesis, Genetic
Genomic Instability
Heterochromatin - genetics
Histones - metabolism
Mice
Mouse Embryonic Stem Cells - metabolism
Telomere Homeostasis - genetics
Transcription Factors - genetics
Transcription Factors - physiology
Transcription, Genetic
pericentromere
embryonic stem cells
heterochromatin
ISSN:1340-2838, 1756-1663, 1756-1663
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Shrnutí:Mouse embryonic stem cells (mESCs) have a remarkable capacity to maintain normal genome stability and karyotype in culture. We previously showed that infrequent bursts of Zscan4 expression (Z4 events) are important for the maintenance of telomere length and genome stability in mESCs. However, the molecular details of Z4 events remain unclear. Here we show that Z4 events involve unexpected transcriptional derepression in heterochromatin regions that usually remain silent. During a Z4 event, we see rapid derepression and rerepression of heterochromatin leading to a burst of transcription that coincides with transient histone hyperacetylation and DNA demethylation, clustering of pericentromeric heterochromatin around the nucleolus, and accumulation of activating and repressive chromatin remodelling complexes. This heterochromatin-based transcriptional activity suggests that mESCs may maintain their extraordinary genome stability at least in part by transiently resetting their heterochromatin.
Bibliografie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Equal contribution first authors.
Edited by Dr Osamu Ohara
ISSN:1340-2838
1756-1663
1756-1663
DOI:10.1093/dnares/dsv013