Application of LC-MS/MS MRM to Determine Staphylococcal Enterotoxins (SEB and SEA) in Milk

Staphylococcus aureus is one of the important aetiological agents of food intoxications in Europe and can cause gastro-enteritis through the production of various staphylococcal enterotoxins (SEs) in foods. Due to their stability and ease of production and dissemination, some SEs have also been stud...

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Veröffentlicht in:Toxins Jg. 8; H. 4; S. 118
Hauptverfasser: Andjelkovic, Mirjana, Tsilia, Varvara, Rajkovic, Andreja, De Cremer, Koen, Van Loco, Joris
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Switzerland MDPI 20.04.2016
MDPI AG
Schlagworte:
Animals
Chromatography, Liquid
Enterotoxins - analysis
Food Contamination - analysis
milk
Milk - chemistry
Peptides - analysis
SEA
SEB
Solid Phase Extraction
Staphylococcus aureus
Staphylococcus aureus enterotoxins
Tandem Mass Spectrometry
UPLC-ESI-MS/MS
Staphylococcus aureus enterotoxins
milk
SEB
UPLC-ESI-MS/MS
SEA
ISSN:2072-6651, 2072-6651
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Zusammenfassung:Staphylococcus aureus is one of the important aetiological agents of food intoxications in Europe and can cause gastro-enteritis through the production of various staphylococcal enterotoxins (SEs) in foods. Due to their stability and ease of production and dissemination, some SEs have also been studied as potential agents for bioterrorism. Therefore, specific and accurate analytical tools are required to detect and quantify SEs. Online solid-phase extraction liquid chromatography electrospray ionization tandem mass spectrometry (online SPE-LC-ESI-MS/MS) based on multiple reaction monitoring (MRM) was used to detect and quantify two types of SE (A and B) spiked in milk and buffer solution. SE extraction and concentration was performed according to the European Screening Method developed by the European Reference Laboratory for Coagulase Positive Staphylococci. Trypsin digests were screened for the presence of SEs using selected proteotypic heavy-labeled peptides as internal standards. SEA and SEB were successfully detected in milk samples using LC-MS/MS in MRM mode. The selected SE peptides were proteotypic for each toxin, allowing the discrimination of SEA and SEB in a single run. The detection limit of SEA and SEB was approximately 8 and 4 ng/g, respectively.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:2072-6651
2072-6651
DOI:10.3390/toxins8040118